Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves
The aim of the present study was to evaluate the prebiotic potential of <i>Plantago major</i> L. leaves water-extractable polysaccharide (PWPs) and its lower molecular fractions. The structure of PWPs was investigated by high pressure anion exchange chromatography (HPAEC), size exclusion...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/10/8/2648 |
| _version_ | 1827718849911324672 |
|---|---|
| author | Paolina Lukova Mariana Nikolova Emmanuel Petit Redouan Elboutachfaiti Tonka Vasileva Plamen Katsarov Hristo Manev Christine Gardarin Guillaume Pierre Philippe Michaud Ilia Iliev Cédric Delattre |
| author_facet | Paolina Lukova Mariana Nikolova Emmanuel Petit Redouan Elboutachfaiti Tonka Vasileva Plamen Katsarov Hristo Manev Christine Gardarin Guillaume Pierre Philippe Michaud Ilia Iliev Cédric Delattre |
| author_sort | Paolina Lukova |
| collection | DOAJ |
| description | The aim of the present study was to evaluate the prebiotic potential of <i>Plantago major</i> L. leaves water-extractable polysaccharide (PWPs) and its lower molecular fractions. The structure of PWPs was investigated by high pressure anion exchange chromatography (HPAEC), size exclusion chromatography coupled with multi-angle laser light scattering detector (SEC-MALLS) and Fourier-transform infrared (FTIR) spectroscopy. The chemical composition and monosaccharide analyses showed that galacturonic acid was the main monosaccharide of PWPs followed by glucose, arabinose, galactose, rhamnose and xylose. FTIR study indicated a strong characteristic absorption peak at 1550 cm<sup>−1</sup> corresponding to the vibration of COO<sup>−</sup> group of galacturonic acid. The PWPs was subjected to hydrolysis using commercial enzymes to obtain <i>P. major</i> low molecular fraction (PLM) which was successively separated by size exclusion chromatography on Biogel P2. PWPs and PLM were examined for in vitro prebiotic activity using various assays. Results gave evidence for changes in optical density of the bacteria cells and pH of the growth medium. A heterofermentative process with a lactate/acetate ratio ranged from 1:1 to 1:5 was observed. The ability of PLM to stimulate the production of certain probiotic bacteria glycohydrolases and to be fermented by <i>Lactobacillus</i> sp. strains was successfully proved. |
| first_indexed | 2024-03-10T20:30:39Z |
| format | Article |
| id | doaj.art-12d3a5d9b4a14411880d4f3fd6e4c62d |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:30:39Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-12d3a5d9b4a14411880d4f3fd6e4c62d2023-11-19T21:22:42ZengMDPI AGApplied Sciences2076-34172020-04-01108264810.3390/app10082648Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. LeavesPaolina Lukova0Mariana Nikolova1Emmanuel Petit2Redouan Elboutachfaiti3Tonka Vasileva4Plamen Katsarov5Hristo Manev6Christine Gardarin7Guillaume Pierre8Philippe Michaud9Ilia Iliev10Cédric Delattre11Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University—Plovdiv, 4002 Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University Paisii Hilendarski, 4000 Plovdiv, BulgariaEA3900 BIOPI Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, 80025 Amiens CEDEX, FranceEA3900 BIOPI Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, 80025 Amiens CEDEX, FranceDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University Paisii Hilendarski, 4000 Plovdiv, BulgariaDepartment of Pharmaceutical sciences, Faculty of Pharmacy, Medical University—Plovdiv, 4002 Plovdiv, BulgariaDepartment of Medical Informatics, Biostatistics and e-Learning, Faculty of Public Health, Medical University—Plovdiv, 4002 Plovdiv, BulgariaUniversité Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, FranceUniversité Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, FranceUniversité Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, FranceDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University Paisii Hilendarski, 4000 Plovdiv, BulgariaUniversité Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, FranceThe aim of the present study was to evaluate the prebiotic potential of <i>Plantago major</i> L. leaves water-extractable polysaccharide (PWPs) and its lower molecular fractions. The structure of PWPs was investigated by high pressure anion exchange chromatography (HPAEC), size exclusion chromatography coupled with multi-angle laser light scattering detector (SEC-MALLS) and Fourier-transform infrared (FTIR) spectroscopy. The chemical composition and monosaccharide analyses showed that galacturonic acid was the main monosaccharide of PWPs followed by glucose, arabinose, galactose, rhamnose and xylose. FTIR study indicated a strong characteristic absorption peak at 1550 cm<sup>−1</sup> corresponding to the vibration of COO<sup>−</sup> group of galacturonic acid. The PWPs was subjected to hydrolysis using commercial enzymes to obtain <i>P. major</i> low molecular fraction (PLM) which was successively separated by size exclusion chromatography on Biogel P2. PWPs and PLM were examined for in vitro prebiotic activity using various assays. Results gave evidence for changes in optical density of the bacteria cells and pH of the growth medium. A heterofermentative process with a lactate/acetate ratio ranged from 1:1 to 1:5 was observed. The ability of PLM to stimulate the production of certain probiotic bacteria glycohydrolases and to be fermented by <i>Lactobacillus</i> sp. strains was successfully proved.https://www.mdpi.com/2076-3417/10/8/2648<i>Plantago major</i> L.polysaccharidesoligosaccharidesenzymatic hydrolysisprebiotic activity |
| spellingShingle | Paolina Lukova Mariana Nikolova Emmanuel Petit Redouan Elboutachfaiti Tonka Vasileva Plamen Katsarov Hristo Manev Christine Gardarin Guillaume Pierre Philippe Michaud Ilia Iliev Cédric Delattre Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves Applied Sciences <i>Plantago major</i> L. polysaccharides oligosaccharides enzymatic hydrolysis prebiotic activity |
| title | Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves |
| title_full | Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves |
| title_fullStr | Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves |
| title_full_unstemmed | Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves |
| title_short | Prebiotic Activity of Poly- and Oligosaccharides Obtained from <i>Plantago major</i> L. Leaves |
| title_sort | prebiotic activity of poly and oligosaccharides obtained from i plantago major i l leaves |
| topic | <i>Plantago major</i> L. polysaccharides oligosaccharides enzymatic hydrolysis prebiotic activity |
| url | https://www.mdpi.com/2076-3417/10/8/2648 |
| work_keys_str_mv | AT paolinalukova prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT mariananikolova prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT emmanuelpetit prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT redouanelboutachfaiti prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT tonkavasileva prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT plamenkatsarov prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT hristomanev prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT christinegardarin prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT guillaumepierre prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT philippemichaud prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT iliailiev prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves AT cedricdelattre prebioticactivityofpolyandoligosaccharidesobtainedfromiplantagomajorilleaves |