Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens
The aim of this study is to follow the gp production in IBDV-vaccinated and challenged birds. The progress of IBDV infection was monitored using anti-VP2 immunocytochemistry, light and transmission electron microscopy. In the medulla of the bursal follicle, the Movat pentachrome staining discovered...
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1689 |
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| author | Balázs Felföldi Zsófia Benyeda Tamás Kovács Nándor Nagy Attila Magyar Imre Oláh |
| author_facet | Balázs Felföldi Zsófia Benyeda Tamás Kovács Nándor Nagy Attila Magyar Imre Oláh |
| author_sort | Balázs Felföldi |
| collection | DOAJ |
| description | The aim of this study is to follow the gp production in IBDV-vaccinated and challenged birds. The progress of IBDV infection was monitored using anti-VP2 immunocytochemistry, light and transmission electron microscopy. In the medulla of the bursal follicle, the Movat pentachrome staining discovered an extracellular glycoprotein (gp) produced by bursal secretory dendritic cells (BSDCs). The secretory granules of BSDCs either discharge resulting in extracellular gp or fuse together forming intracellular corpuscles. The double fate of granules suggests a dual function of BSDCs: (a.) For the discharged granules, gp contributes to the medullary microenvironment (ME). (b.) The intracellular corpuscles may be the sign of BSDC transformation to a macrophage-like cell (Mal). Infectious bursal disease virus (IBDV) infection accelerates the BSDC transformation to Mal. The decreased number of BSDCs is feedback for the precursor cells of BSDCs lodging in the cortico-medullary epithelial arches (CMEA), where they proliferate. Opening the CMEA, the precursor cells enter the medulla, and differentiate to immature BSDCs. The virus uptake in the corpuscles prevents the granular discharge resulting in the absence of gp and alteration in ME. In vaccine-take birds, the mitotic rate of BSDC precursor cells cannot restore the precursor pool; therefore, in the case of IBDV challenge, the number of newly formed BSDCs is too low for outbreak of clinical disease. The BSDCs, as a primary target of IBDV, may contribute to the long-lasting immunosuppressive status of IBDV-infected chickens. |
| first_indexed | 2024-03-09T09:47:32Z |
| format | Article |
| id | doaj.art-12d6d0e43b9a4ed4a0682b2899ce6706 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T09:47:32Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-12d6d0e43b9a4ed4a0682b2899ce67062023-12-02T00:26:28ZengMDPI AGViruses1999-49152022-07-01148168910.3390/v14081689Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected ChickensBalázs Felföldi0Zsófia Benyeda1Tamás Kovács2Nándor Nagy3Attila Magyar4Imre Oláh5Ceva-Phylaxia Ltd., 1107 Budapest, HungaryBiovo Animal Health Ltd., 7700 Mohács, HungaryDepartment of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, HungaryDepartment of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, HungaryDepartment of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, HungaryDepartment of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, HungaryThe aim of this study is to follow the gp production in IBDV-vaccinated and challenged birds. The progress of IBDV infection was monitored using anti-VP2 immunocytochemistry, light and transmission electron microscopy. In the medulla of the bursal follicle, the Movat pentachrome staining discovered an extracellular glycoprotein (gp) produced by bursal secretory dendritic cells (BSDCs). The secretory granules of BSDCs either discharge resulting in extracellular gp or fuse together forming intracellular corpuscles. The double fate of granules suggests a dual function of BSDCs: (a.) For the discharged granules, gp contributes to the medullary microenvironment (ME). (b.) The intracellular corpuscles may be the sign of BSDC transformation to a macrophage-like cell (Mal). Infectious bursal disease virus (IBDV) infection accelerates the BSDC transformation to Mal. The decreased number of BSDCs is feedback for the precursor cells of BSDCs lodging in the cortico-medullary epithelial arches (CMEA), where they proliferate. Opening the CMEA, the precursor cells enter the medulla, and differentiate to immature BSDCs. The virus uptake in the corpuscles prevents the granular discharge resulting in the absence of gp and alteration in ME. In vaccine-take birds, the mitotic rate of BSDC precursor cells cannot restore the precursor pool; therefore, in the case of IBDV challenge, the number of newly formed BSDCs is too low for outbreak of clinical disease. The BSDCs, as a primary target of IBDV, may contribute to the long-lasting immunosuppressive status of IBDV-infected chickens.https://www.mdpi.com/1999-4915/14/8/1689bursal secretory dendritic cell (BSDC)glycoprotein (gp)IBDV infectionvaccination effect on gp production |
| spellingShingle | Balázs Felföldi Zsófia Benyeda Tamás Kovács Nándor Nagy Attila Magyar Imre Oláh Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens Viruses bursal secretory dendritic cell (BSDC) glycoprotein (gp) IBDV infection vaccination effect on gp production |
| title | Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens |
| title_full | Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens |
| title_fullStr | Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens |
| title_full_unstemmed | Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens |
| title_short | Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens |
| title_sort | glycoprotein production by bursal secretory dendritic cells in normal vaccinated and infectious bursal disease virus ibdv infected chickens |
| topic | bursal secretory dendritic cell (BSDC) glycoprotein (gp) IBDV infection vaccination effect on gp production |
| url | https://www.mdpi.com/1999-4915/14/8/1689 |
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