Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations?
Antifungal susceptibility testing is important in guiding patient therapy due to an increasing number of resistant <i>Candida</i> isolates. In the clinical strain collection of the Austrian resistance report (AURES), a high number of micafungin-resistant <i>C. albicans</i> is...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/2/93 |
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| author | Kathrin Spettel Sonia Galazka Richard Kriz Iris Camp Birgit Willinger |
| author_facet | Kathrin Spettel Sonia Galazka Richard Kriz Iris Camp Birgit Willinger |
| author_sort | Kathrin Spettel |
| collection | DOAJ |
| description | Antifungal susceptibility testing is important in guiding patient therapy due to an increasing number of resistant <i>Candida</i> isolates. In the clinical strain collection of the Austrian resistance report (AURES), a high number of micafungin-resistant <i>C. albicans</i> isolates (18.2% 49/269) was detected in seven different centres in Austria from 2011–2016. Most of these isolates showed a micafungin MIC value that was just above the clinical breakpoint (CB) established by EUCAST (0.016 mg/L). The aim of this study was to analyse whether <i>C. albicans</i> strains showing a micafungin MIC value of 1–2 dilutions above the CB (0.032 mg/L and 0.064 mg/L) are associated with mutations in <i>FKS1</i> hotspot (HS) regions. 115 <i>C. albicans</i> candidemia strains showing a micafungin MIC one or two dilutions above the EUCAST CB (0.032 mg/L and 0.064 mg/L) were categorized as borderline resistant and screened for mutations in <i>FKS1</i> HS1, HS2, and HS3 regions, which are known locations for the development of echinocandin resistance. For this purpose, we implemented targeted resequencing utilizing a next generation sequencing technology. No missense mutations could be detected in <i>FKS1</i> HS1, HS2, and HS3 in any of the 115 isolates, which indicated that resistance conferred by alteration of <i>FKS1</i> seems unlikely. |
| first_indexed | 2024-03-09T03:28:03Z |
| format | Article |
| id | doaj.art-12d8e99dd98a4efdaaafd2c092d1baab |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T03:28:03Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-12d8e99dd98a4efdaaafd2c092d1baab2023-12-03T14:58:27ZengMDPI AGJournal of Fungi2309-608X2021-01-01729310.3390/jof7020093Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations?Kathrin Spettel0Sonia Galazka1Richard Kriz2Iris Camp3Birgit Willinger4Division of Clinical Microbiology, Department of Laboratory Medicine, Medical University of Vienna, 1090 Vienna, AustriaDivision of Clinical Microbiology, Department of Laboratory Medicine, Medical University of Vienna, 1090 Vienna, AustriaDivision of Infectious Diseases and Tropical Medicine, Department of Medicine I, Medical University of Vienna, 1090 Vienna, AustriaDivision of Clinical Microbiology, Department of Laboratory Medicine, Medical University of Vienna, 1090 Vienna, AustriaDivision of Clinical Microbiology, Department of Laboratory Medicine, Medical University of Vienna, 1090 Vienna, AustriaAntifungal susceptibility testing is important in guiding patient therapy due to an increasing number of resistant <i>Candida</i> isolates. In the clinical strain collection of the Austrian resistance report (AURES), a high number of micafungin-resistant <i>C. albicans</i> isolates (18.2% 49/269) was detected in seven different centres in Austria from 2011–2016. Most of these isolates showed a micafungin MIC value that was just above the clinical breakpoint (CB) established by EUCAST (0.016 mg/L). The aim of this study was to analyse whether <i>C. albicans</i> strains showing a micafungin MIC value of 1–2 dilutions above the CB (0.032 mg/L and 0.064 mg/L) are associated with mutations in <i>FKS1</i> hotspot (HS) regions. 115 <i>C. albicans</i> candidemia strains showing a micafungin MIC one or two dilutions above the EUCAST CB (0.032 mg/L and 0.064 mg/L) were categorized as borderline resistant and screened for mutations in <i>FKS1</i> HS1, HS2, and HS3 regions, which are known locations for the development of echinocandin resistance. For this purpose, we implemented targeted resequencing utilizing a next generation sequencing technology. No missense mutations could be detected in <i>FKS1</i> HS1, HS2, and HS3 in any of the 115 isolates, which indicated that resistance conferred by alteration of <i>FKS1</i> seems unlikely.https://www.mdpi.com/2309-608X/7/2/93<i>FKS1</i> mutationsechinocandin resistance<i>Candida albicans</i>next-generation sequencingmicafungin |
| spellingShingle | Kathrin Spettel Sonia Galazka Richard Kriz Iris Camp Birgit Willinger Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations? Journal of Fungi <i>FKS1</i> mutations echinocandin resistance <i>Candida albicans</i> next-generation sequencing micafungin |
| title | Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations? |
| title_full | Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations? |
| title_fullStr | Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations? |
| title_full_unstemmed | Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations? |
| title_short | Do <i>Candida albicans</i> Isolates with Borderline Resistant Micafungin MICs Always Harbor <i>FKS1</i> Hot Spot Mutations? |
| title_sort | do i candida albicans i isolates with borderline resistant micafungin mics always harbor i fks1 i hot spot mutations |
| topic | <i>FKS1</i> mutations echinocandin resistance <i>Candida albicans</i> next-generation sequencing micafungin |
| url | https://www.mdpi.com/2309-608X/7/2/93 |
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