Host intestinal biomarker identification in a gut leakage model in broilers
Abstract Intestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-019-0663-x |
| _version_ | 1819074914467971072 |
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| author | Fien De Meyer Venessa Eeckhaut Richard Ducatelle Maarten Dhaenens Simon Daled Annelike Dedeurwaerder Maarten De Gussem Freddy Haesebrouck Dieter Deforce Filip Van Immerseel |
| author_facet | Fien De Meyer Venessa Eeckhaut Richard Ducatelle Maarten Dhaenens Simon Daled Annelike Dedeurwaerder Maarten De Gussem Freddy Haesebrouck Dieter Deforce Filip Van Immerseel |
| author_sort | Fien De Meyer |
| collection | DOAJ |
| description | Abstract Intestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The aim of the study was to identify host protein biomarkers for intestinal inflammation and intestinal barrier damage. Proteomic analysis was conducted on ileal and colonic content samples of broilers under an experimental gut damage and inflammation model. Effects of the challenge treatment resulted in a worse gut condition based on macroscopic gut appearance (p < 0.0001). Also microscopic changes such as shortening of the villi and increased crypt depth (p < 0.0001) as well as higher infiltration of T-lymphocytes (p < 0.0001) were seen in the duodenal tissue of challenged animals. Several candidate proteins associated with inflammation, serum leakage and/or tissue damage were identified with an increased abundance in intestinal content of challenged animals (p < 0.05). Conversely, brush border enzymes were less abundant in intestinal content of challenged animals (p < 0.05). These candidate biomarkers have potential to be used in the field for detection of gut barrier failure in broilers. |
| first_indexed | 2024-12-21T18:17:05Z |
| format | Article |
| id | doaj.art-12da8d2d0f164874840e00a386e8fe8d |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-21T18:17:05Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-12da8d2d0f164874840e00a386e8fe8d2022-12-21T18:54:38ZengBMCVeterinary Research1297-97162019-06-0150111410.1186/s13567-019-0663-xHost intestinal biomarker identification in a gut leakage model in broilersFien De Meyer0Venessa Eeckhaut1Richard Ducatelle2Maarten Dhaenens3Simon Daled4Annelike Dedeurwaerder5Maarten De Gussem6Freddy Haesebrouck7Dieter Deforce8Filip Van Immerseel9Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityLaboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityLaboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityPoulpharm BVBAPoulpharm BVBADepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityLaboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityAbstract Intestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The aim of the study was to identify host protein biomarkers for intestinal inflammation and intestinal barrier damage. Proteomic analysis was conducted on ileal and colonic content samples of broilers under an experimental gut damage and inflammation model. Effects of the challenge treatment resulted in a worse gut condition based on macroscopic gut appearance (p < 0.0001). Also microscopic changes such as shortening of the villi and increased crypt depth (p < 0.0001) as well as higher infiltration of T-lymphocytes (p < 0.0001) were seen in the duodenal tissue of challenged animals. Several candidate proteins associated with inflammation, serum leakage and/or tissue damage were identified with an increased abundance in intestinal content of challenged animals (p < 0.05). Conversely, brush border enzymes were less abundant in intestinal content of challenged animals (p < 0.05). These candidate biomarkers have potential to be used in the field for detection of gut barrier failure in broilers.http://link.springer.com/article/10.1186/s13567-019-0663-x |
| spellingShingle | Fien De Meyer Venessa Eeckhaut Richard Ducatelle Maarten Dhaenens Simon Daled Annelike Dedeurwaerder Maarten De Gussem Freddy Haesebrouck Dieter Deforce Filip Van Immerseel Host intestinal biomarker identification in a gut leakage model in broilers Veterinary Research |
| title | Host intestinal biomarker identification in a gut leakage model in broilers |
| title_full | Host intestinal biomarker identification in a gut leakage model in broilers |
| title_fullStr | Host intestinal biomarker identification in a gut leakage model in broilers |
| title_full_unstemmed | Host intestinal biomarker identification in a gut leakage model in broilers |
| title_short | Host intestinal biomarker identification in a gut leakage model in broilers |
| title_sort | host intestinal biomarker identification in a gut leakage model in broilers |
| url | http://link.springer.com/article/10.1186/s13567-019-0663-x |
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