Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study
Delay in the time-to-positivity of a peripheral blood culture (PBC), the gold standard for early onset neonatal sepsis (EOS) diagnosis, has resulted in excessive use of antibiotics. In this study, we evaluate the potential of the rapid Molecular Culture (MC) assay for quick EOS diagnosis. In the fir...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/960 |
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| author | Thomas Dierikx Andries Budding Martine Bos Henriëtte van Laerhoven Sophie van der Schoor Hendrik Niemarkt Marc Benninga Anton van Kaam Douwe Visser Tim de Meij |
| author_facet | Thomas Dierikx Andries Budding Martine Bos Henriëtte van Laerhoven Sophie van der Schoor Hendrik Niemarkt Marc Benninga Anton van Kaam Douwe Visser Tim de Meij |
| author_sort | Thomas Dierikx |
| collection | DOAJ |
| description | Delay in the time-to-positivity of a peripheral blood culture (PBC), the gold standard for early onset neonatal sepsis (EOS) diagnosis, has resulted in excessive use of antibiotics. In this study, we evaluate the potential of the rapid Molecular Culture (MC) assay for quick EOS diagnosis. In the first part of this study, known positive and spiked blood samples were used to assess the performance of MC. In the in vivo clinical study, the second part of this study, all infants receiving antibiotics for suspicion of EOS were included. At initial EOS suspicion, a blood sample was collected for PBC and MC. MC was able to detect bacteria present in the spiked samples even when the bacterial load was low. In the clinical study, MC was positive in one infant with clinical EOS (<i>Enterococcus faecalis</i>) that was not detected by PBC. Additionally, MC was positive in two infants without clinical sepsis (<i>Streptococcus mitis</i> and multiple species), referred to as contamination. The other 37 samples were negative both by MC and PBC. MC seems to be able to detect bacteria even when the bacterial load is low. The majority of MC and PBC results were comparable and the risk for contamination and false positive MC results seems to be limited. Since MC can generate results within 4 h following sampling compared with 36–72 h in PBC, MC may have the potential to replace conventional PBC in EOS diagnostics in order to guide clinicians on when to discontinue antibiotic therapy several hours after birth. |
| first_indexed | 2024-03-11T04:43:13Z |
| format | Article |
| id | doaj.art-12db16e2cf224180bc651fbbccfd8565 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:13Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-12db16e2cf224180bc651fbbccfd85652023-11-17T20:32:59ZengMDPI AGMicroorganisms2076-26072023-04-0111496010.3390/microorganisms11040960Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle StudyThomas Dierikx0Andries Budding1Martine Bos2Henriëtte van Laerhoven3Sophie van der Schoor4Hendrik Niemarkt5Marc Benninga6Anton van Kaam7Douwe Visser8Tim de Meij9Department of Pediatric Gastroenterology, Amsterdam UMC Location University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsInbiome, 1098 XG Amsterdam, The NetherlandsInbiome, 1098 XG Amsterdam, The NetherlandsDepartment of Pediatrics and Neonatology, OLVG, 1061 AE Amsterdam, The NetherlandsDepartment of Pediatrics and Neonatology, OLVG, 1061 AE Amsterdam, The NetherlandsDepartment of Neonatology, Máxima Medisch Centrum, 5504 DB Veldhoven, The NetherlandsDepartment of Pediatric Gastroenterology, Amsterdam UMC Location University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Neonatology, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsDepartment of Neonatology, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsDepartment of Pediatric Gastroenterology, Amsterdam UMC Location University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDelay in the time-to-positivity of a peripheral blood culture (PBC), the gold standard for early onset neonatal sepsis (EOS) diagnosis, has resulted in excessive use of antibiotics. In this study, we evaluate the potential of the rapid Molecular Culture (MC) assay for quick EOS diagnosis. In the first part of this study, known positive and spiked blood samples were used to assess the performance of MC. In the in vivo clinical study, the second part of this study, all infants receiving antibiotics for suspicion of EOS were included. At initial EOS suspicion, a blood sample was collected for PBC and MC. MC was able to detect bacteria present in the spiked samples even when the bacterial load was low. In the clinical study, MC was positive in one infant with clinical EOS (<i>Enterococcus faecalis</i>) that was not detected by PBC. Additionally, MC was positive in two infants without clinical sepsis (<i>Streptococcus mitis</i> and multiple species), referred to as contamination. The other 37 samples were negative both by MC and PBC. MC seems to be able to detect bacteria even when the bacterial load is low. The majority of MC and PBC results were comparable and the risk for contamination and false positive MC results seems to be limited. Since MC can generate results within 4 h following sampling compared with 36–72 h in PBC, MC may have the potential to replace conventional PBC in EOS diagnostics in order to guide clinicians on when to discontinue antibiotic therapy several hours after birth.https://www.mdpi.com/2076-2607/11/4/960early onset sepsisneonatesmolecular culturediagnosisIS-pro |
| spellingShingle | Thomas Dierikx Andries Budding Martine Bos Henriëtte van Laerhoven Sophie van der Schoor Hendrik Niemarkt Marc Benninga Anton van Kaam Douwe Visser Tim de Meij Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study Microorganisms early onset sepsis neonates molecular culture diagnosis IS-pro |
| title | Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study |
| title_full | Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study |
| title_fullStr | Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study |
| title_full_unstemmed | Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study |
| title_short | Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study |
| title_sort | potential of molecular culture in early onset neonatal sepsis diagnosis a proof of principle study |
| topic | early onset sepsis neonates molecular culture diagnosis IS-pro |
| url | https://www.mdpi.com/2076-2607/11/4/960 |
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