Effects of Hesperidin as a Radioprotector on Apoptosis in Rat Peripheral Blood Lymphocytes after Gamma Radiation
Introduction: Hesperidin (HES), as the most abundant flavonoid existing in the citrus, is widely used by human daily. The radio-protective effects of Hesperidin have been confirmed in various measurement systems. This study aimed to evaluate the effects of Hesperidin on the changes in the apoptos...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2016-12-01
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| Series: | Journal of Biomedical Physics and Engineering |
| Subjects: | |
| Online Access: | http://www.jbpe.org/Journal_OJS/JBPE/index.php/jbpe/article/view/543/312 |
| Summary: | Introduction: Hesperidin (HES), as the most abundant flavonoid existing in the
citrus, is widely used by human daily. The radio-protective effects of Hesperidin have
been confirmed in various measurement systems. This study aimed to evaluate the effects
of Hesperidin on the changes in the apoptosis level and expression of apoptotic
genes target (bax, bcl-2 and ration of bax/bcl-2) in the peripheral blood lymphocytes
of male rats after gamma radiation.
Materials and Methods: 64 male rats were divided into eight groups: Control,
HES (100 mg/kg b.w, orally, 7 days), whole body irradiation with 2 and 8Gy, preadministrated
with 50 and 100 mg/kg body weight of Hesperidin for 7 days before irradiation
with 2 and 8 Gy. 24 hours after radiation, apoptotic lymphocytes were evaluated
using PE Annexin V Apoptosis detection I kit and the levels of mRNA for bax
and bcl-2 were evaluated by real time reverse transcription polymerase chain reaction.
Results: A significant reduction in apoptosis of the lymphocytes was demonstrated
in group animals receiving 8 Gy compared to the group which received 2 Gy irradiation
(p<0.0001). However, apoptosis significantly increased in group of rats who
received Hesp before irradiation (p<0.05). The increase of apoptosis by Hesperidin
administration can be attributed to the decreased expression of bax and significantly
reduced expression of bcl-2 and finally increasing the ration of bax/bcl-2.
Conclusion: The results suggest that administration of 50 and 100 mg/kg of Hesperidin
induces apoptotic effects by changing expression level of bax, bcl-2 and also
the ratio of bax/bcl2. |
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| ISSN: | 2251-7200 2251-7200 |