Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells
Abstract Background The aim of this study was to evaluate the indirect effects of residual silver nanoparticles (AgNPs) gel on human dental pulp stromal cells (DPSCs). Methods Ninety-five dentin discs (4x4x1 mm) were prepared from freshly extracted human single-rooted teeth following institutional e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-022-02542-2 |
| _version_ | 1811208020359643136 |
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| author | Ahmed Mahmoud Sybel Moussa Rania El Backly Reem El-Gendy |
| author_facet | Ahmed Mahmoud Sybel Moussa Rania El Backly Reem El-Gendy |
| author_sort | Ahmed Mahmoud |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to evaluate the indirect effects of residual silver nanoparticles (AgNPs) gel on human dental pulp stromal cells (DPSCs). Methods Ninety-five dentin discs (4x4x1 mm) were prepared from freshly extracted human single-rooted teeth following institutional ethical approval and informed consent. Samples were cleaned, autoclaved, and treated with: 1.5%NaOCl, Saline and 17% EDTA then randomly assigned to 5 groups that received 50 μl of one of the following treatments: 0.01%AgNPs, 0.015%AgNPs, 0.02%AgNPs, Calcium hydroxide (Ca (OH)2) or no treatment for 1 week. Discs were washed with Saline and 17%EDTA then seeded with DPSCs and incubated for 3 and 7 days. At 24 hours unattached cells were collected and counted. At each time point cytotoxicity (LDH assay), cell viability (live/dead staining and confocal microscopy) and cell proliferation (WST1 assay) were assessed. All experiments were repeated a minimum of 3 times using DPSCs isolated from 3 different donors for each time point assessed (n = 9/group). Statistical analysis was done using One-Way ANOVA followed by Tukey’s test and Kruskal Wallis followed by post-hoc comparisons with significance set at p ≤ 0.05. Results After 24 hours, the percentage of DPSCs attachment ranged between 92.66% ±4.54 and 95.08% ±1.44 with no significant difference between groups (P = 0.126). Cell viability was ≥92% at 24 hours for all groups. However this percentage dropped to less than 60% at 3 days then started to rise again at 7 days. There was no significant difference in cytotoxicity between different groups at all time points except for 0.01%AgNPs group which had the highest cytotoxicity. DPSCs proliferation increased significantly from 3 to 7 days in all groups except for Ca (OH)2 which showed lower proliferation rates at both 3 (45.89%) and 7 days (79.25%). Conclusion Dentin discs treated for 7 days with concentrations of AgNPs gel (0.01–0.02%) allowed more than 90% DPSCs cell attachment after 24 hours. The cytotoxicity and proliferation of DPSCs in response to AgNPs gel were comparable to those with calcium hydroxide. This suggests that AgNPs gel may represent a promising future candidate for clinical use in regenerative endodontics. However, its effects may be concentration-dependent warranting further investigation. |
| first_indexed | 2024-04-12T04:15:12Z |
| format | Article |
| id | doaj.art-12dd0ca66ee146f699b8fd20c82477c2 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-12T04:15:12Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-12dd0ca66ee146f699b8fd20c82477c22022-12-22T03:48:25ZengBMCBMC Oral Health1472-68312022-11-0122111410.1186/s12903-022-02542-2Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cellsAhmed Mahmoud0Sybel Moussa1Rania El Backly2Reem El-Gendy3Endodontics, Faculty of Dentistry, Kafr El-Sheikh UniversityEndodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityEndodontics, Conservative Dentistry Department and tissue engineering laboratories, Faculty of Dentistry, Alexandria UniversityDivision of Oral Biology, University of Leeds, School of DentistryAbstract Background The aim of this study was to evaluate the indirect effects of residual silver nanoparticles (AgNPs) gel on human dental pulp stromal cells (DPSCs). Methods Ninety-five dentin discs (4x4x1 mm) were prepared from freshly extracted human single-rooted teeth following institutional ethical approval and informed consent. Samples were cleaned, autoclaved, and treated with: 1.5%NaOCl, Saline and 17% EDTA then randomly assigned to 5 groups that received 50 μl of one of the following treatments: 0.01%AgNPs, 0.015%AgNPs, 0.02%AgNPs, Calcium hydroxide (Ca (OH)2) or no treatment for 1 week. Discs were washed with Saline and 17%EDTA then seeded with DPSCs and incubated for 3 and 7 days. At 24 hours unattached cells were collected and counted. At each time point cytotoxicity (LDH assay), cell viability (live/dead staining and confocal microscopy) and cell proliferation (WST1 assay) were assessed. All experiments were repeated a minimum of 3 times using DPSCs isolated from 3 different donors for each time point assessed (n = 9/group). Statistical analysis was done using One-Way ANOVA followed by Tukey’s test and Kruskal Wallis followed by post-hoc comparisons with significance set at p ≤ 0.05. Results After 24 hours, the percentage of DPSCs attachment ranged between 92.66% ±4.54 and 95.08% ±1.44 with no significant difference between groups (P = 0.126). Cell viability was ≥92% at 24 hours for all groups. However this percentage dropped to less than 60% at 3 days then started to rise again at 7 days. There was no significant difference in cytotoxicity between different groups at all time points except for 0.01%AgNPs group which had the highest cytotoxicity. DPSCs proliferation increased significantly from 3 to 7 days in all groups except for Ca (OH)2 which showed lower proliferation rates at both 3 (45.89%) and 7 days (79.25%). Conclusion Dentin discs treated for 7 days with concentrations of AgNPs gel (0.01–0.02%) allowed more than 90% DPSCs cell attachment after 24 hours. The cytotoxicity and proliferation of DPSCs in response to AgNPs gel were comparable to those with calcium hydroxide. This suggests that AgNPs gel may represent a promising future candidate for clinical use in regenerative endodontics. However, its effects may be concentration-dependent warranting further investigation.https://doi.org/10.1186/s12903-022-02542-2Regenerative endodontic proceduresDental pulp stromal cellsIntra-canal medicamentsSilver nanoparticlesCalcium hydroxide |
| spellingShingle | Ahmed Mahmoud Sybel Moussa Rania El Backly Reem El-Gendy Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells BMC Oral Health Regenerative endodontic procedures Dental pulp stromal cells Intra-canal medicaments Silver nanoparticles Calcium hydroxide |
| title | Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells |
| title_full | Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells |
| title_fullStr | Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells |
| title_full_unstemmed | Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells |
| title_short | Investigating the residual effect of silver nanoparticles gel as an intra-canal medicament on dental pulp stromal cells |
| title_sort | investigating the residual effect of silver nanoparticles gel as an intra canal medicament on dental pulp stromal cells |
| topic | Regenerative endodontic procedures Dental pulp stromal cells Intra-canal medicaments Silver nanoparticles Calcium hydroxide |
| url | https://doi.org/10.1186/s12903-022-02542-2 |
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