Pathogenicity Determinants of and Their Association to Its Aggressiveness on

White rot or stem rot caused by Sclerotinia sclerotiorum is one of the most destructive fungal diseases that have become a serious threat to the successful cultivation of oilseed Brassicas. The study was designed with an aim to investigate the association between the pathogenic aggressiveness and pa...

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Main Authors: Rupeet Gill, Prabhjodh S. Sandhu, Sanjula Sharma, Pankaj Sharma
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2021-08-01
Series:The Plant Pathology Journal
Subjects:
Online Access:http://www.ppjonline.org/upload/pdf/PPJ-OA-03-2021-0036.pdf
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author Rupeet Gill
Prabhjodh S. Sandhu
Sanjula Sharma
Pankaj Sharma
author_facet Rupeet Gill
Prabhjodh S. Sandhu
Sanjula Sharma
Pankaj Sharma
author_sort Rupeet Gill
collection DOAJ
description White rot or stem rot caused by Sclerotinia sclerotiorum is one of the most destructive fungal diseases that have become a serious threat to the successful cultivation of oilseed Brassicas. The study was designed with an aim to investigate the association between the pathogenic aggressiveness and pathogenicity determinants of this pathogen specifically in Brassica for the first time. For this, a total of 58 isolates of S. sclerotiorum from different geographical regions were collected and purified. These isolates were inoculated on a Brassica juncea cv. RL-1359 and they exhibited high level of variation in their disease progression. The isolates were grouped and then 24 isolates were selected for the biochemical analysis of pathogenicity determinants. The isolates varied significantly with respect to their total organic acids, oxalic acid production and pectin methyl esterase and polygalacturonase activity. The oxalic acid production corresponded to the disease progression of the isolates; the isolates with higher oxalic acid production were the more aggressive ones and vice-versa. This is, in our knowledge, the first study to establish a correlation between oxalic acid production and pathogenic aggressiveness of S. sclerotiorum on B. juncea. However, the pectinases’ enzyme activity did not follow the trend as of disease progression. These suggest an indispensable role of oxalic acid in pathogenicity of the fungus and the potential to be used as biochemical marker for preliminary assessment of pathogenic aggressiveness of various isolates before incorporating them in a breeding program.
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spelling doaj.art-12dd5d6397d448a59b693c0fe99aad812022-12-22T01:43:07ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-08-0137436537410.5423/PPJ.OA.03.2021.00362261Pathogenicity Determinants of and Their Association to Its Aggressiveness onRupeet Gill0Prabhjodh S. Sandhu1Sanjula Sharma2Pankaj Sharma3 Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India DBT Centre of Excellence on Brassicas, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141004, Punjab, India DBT Centre of Excellence on Brassicas, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141004, Punjab, India DBT Centre of Excellence on Brassicas, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141004, Punjab, IndiaWhite rot or stem rot caused by Sclerotinia sclerotiorum is one of the most destructive fungal diseases that have become a serious threat to the successful cultivation of oilseed Brassicas. The study was designed with an aim to investigate the association between the pathogenic aggressiveness and pathogenicity determinants of this pathogen specifically in Brassica for the first time. For this, a total of 58 isolates of S. sclerotiorum from different geographical regions were collected and purified. These isolates were inoculated on a Brassica juncea cv. RL-1359 and they exhibited high level of variation in their disease progression. The isolates were grouped and then 24 isolates were selected for the biochemical analysis of pathogenicity determinants. The isolates varied significantly with respect to their total organic acids, oxalic acid production and pectin methyl esterase and polygalacturonase activity. The oxalic acid production corresponded to the disease progression of the isolates; the isolates with higher oxalic acid production were the more aggressive ones and vice-versa. This is, in our knowledge, the first study to establish a correlation between oxalic acid production and pathogenic aggressiveness of S. sclerotiorum on B. juncea. However, the pectinases’ enzyme activity did not follow the trend as of disease progression. These suggest an indispensable role of oxalic acid in pathogenicity of the fungus and the potential to be used as biochemical marker for preliminary assessment of pathogenic aggressiveness of various isolates before incorporating them in a breeding program.http://www.ppjonline.org/upload/pdf/PPJ-OA-03-2021-0036.pdfoxalic acidpectin methyl esterasepolygalacturonase
spellingShingle Rupeet Gill
Prabhjodh S. Sandhu
Sanjula Sharma
Pankaj Sharma
Pathogenicity Determinants of and Their Association to Its Aggressiveness on
The Plant Pathology Journal
oxalic acid
pectin methyl esterase
polygalacturonase
title Pathogenicity Determinants of and Their Association to Its Aggressiveness on
title_full Pathogenicity Determinants of and Their Association to Its Aggressiveness on
title_fullStr Pathogenicity Determinants of and Their Association to Its Aggressiveness on
title_full_unstemmed Pathogenicity Determinants of and Their Association to Its Aggressiveness on
title_short Pathogenicity Determinants of and Their Association to Its Aggressiveness on
title_sort pathogenicity determinants of and their association to its aggressiveness on
topic oxalic acid
pectin methyl esterase
polygalacturonase
url http://www.ppjonline.org/upload/pdf/PPJ-OA-03-2021-0036.pdf
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AT prabhjodhssandhu pathogenicitydeterminantsofandtheirassociationtoitsaggressivenesson
AT sanjulasharma pathogenicitydeterminantsofandtheirassociationtoitsaggressivenesson
AT pankajsharma pathogenicitydeterminantsofandtheirassociationtoitsaggressivenesson