Signal mediators at induction of heat resistance of wheat plantlets by short-term heating
The effects of functional interplay of calcium ions, reactive oxygen species (ROS) and nitric oxide (NO) in the cells of wheat plantlets roots (Triticum aestivum L.) at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 °С during 1 minu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-12-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/12/Karpets_6_15.pdf |
| _version_ | 1797422276229988352 |
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| author | Yu. V. Karpets Yu. E. Kolupaev T. O. Yastreb |
| author_facet | Yu. V. Karpets Yu. E. Kolupaev T. O. Yastreb |
| author_sort | Yu. V. Karpets |
| collection | DOAJ |
| description | The effects of functional interplay of calcium ions, reactive oxygen species (ROS) and nitric oxide (NO) in the cells of wheat plantlets roots (Triticum aestivum L.) at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 °С during 1 minute) have been investigated. The transitional increase of NO and H2O2 content, invoked by heating, was suppressed by the treatment of plantlets with the antagonists of calcium EGTA (chelator of exocellular calcium), lanthanum chloride (blocker of calcium channels of various types) and neomycin (inhibitor of phosphatidylinositol-dependent phospholipase C). The rise of hydrogen peroxide content, caused by hardening, was partially suppressed by the action of inhibitors of nitrate reductase (sodium wolframate) and NO-synthase (NG-nitro-L-arginine methyl ester – L-NAME), and the increasing of nitric oxide content was suppressed by the treatment of plants with the antioxidant ionol and with the scavenger of hydrogen peroxide (dimethylthiourea). These compounds and antagonists of calcium also partially removed the effect of the rise of plantlets’ heat resistance, invoked by hardening heating. The conclusion on calcium’s role in the activation of enzymatic systems, generating reactive oxygen species and nitric oxide, and on the functional interplay of these signal mediators at the induction of heat resistance of plantlets by hardening heating is made. |
| first_indexed | 2024-03-09T07:29:52Z |
| format | Article |
| id | doaj.art-12df72a294694541ba200ef215f66286 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:29:52Z |
| publishDate | 2015-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-12df72a294694541ba200ef215f662862023-12-03T06:26:47ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032015-12-0187610411210.15407/ubj87.06.104Signal mediators at induction of heat resistance of wheat plantlets by short-term heatingYu. V. Karpets0Yu. E. Kolupaev1T. O. Yastreb2V. V. Dokuchaev Kharkiv National Agrarian University, Ukraine;V. V. Dokuchaev Kharkiv National Agrarian University, Ukraine;V. V. Dokuchaev Kharkiv National Agrarian University, Ukraine;The effects of functional interplay of calcium ions, reactive oxygen species (ROS) and nitric oxide (NO) in the cells of wheat plantlets roots (Triticum aestivum L.) at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 °С during 1 minute) have been investigated. The transitional increase of NO and H2O2 content, invoked by heating, was suppressed by the treatment of plantlets with the antagonists of calcium EGTA (chelator of exocellular calcium), lanthanum chloride (blocker of calcium channels of various types) and neomycin (inhibitor of phosphatidylinositol-dependent phospholipase C). The rise of hydrogen peroxide content, caused by hardening, was partially suppressed by the action of inhibitors of nitrate reductase (sodium wolframate) and NO-synthase (NG-nitro-L-arginine methyl ester – L-NAME), and the increasing of nitric oxide content was suppressed by the treatment of plants with the antioxidant ionol and with the scavenger of hydrogen peroxide (dimethylthiourea). These compounds and antagonists of calcium also partially removed the effect of the rise of plantlets’ heat resistance, invoked by hardening heating. The conclusion on calcium’s role in the activation of enzymatic systems, generating reactive oxygen species and nitric oxide, and on the functional interplay of these signal mediators at the induction of heat resistance of plantlets by hardening heating is made.http://ukrbiochemjournal.org/wp-content/uploads/2015/12/Karpets_6_15.pdfcalciumheat resistancenitric oxidereactive oxygen speciesstress signallingTriticum aestivum L. |
| spellingShingle | Yu. V. Karpets Yu. E. Kolupaev T. O. Yastreb Signal mediators at induction of heat resistance of wheat plantlets by short-term heating The Ukrainian Biochemical Journal calcium heat resistance nitric oxide reactive oxygen species stress signalling Triticum aestivum L. |
| title | Signal mediators at induction of heat resistance of wheat plantlets by short-term heating |
| title_full | Signal mediators at induction of heat resistance of wheat plantlets by short-term heating |
| title_fullStr | Signal mediators at induction of heat resistance of wheat plantlets by short-term heating |
| title_full_unstemmed | Signal mediators at induction of heat resistance of wheat plantlets by short-term heating |
| title_short | Signal mediators at induction of heat resistance of wheat plantlets by short-term heating |
| title_sort | signal mediators at induction of heat resistance of wheat plantlets by short term heating |
| topic | calcium heat resistance nitric oxide reactive oxygen species stress signalling Triticum aestivum L. |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/12/Karpets_6_15.pdf |
| work_keys_str_mv | AT yuvkarpets signalmediatorsatinductionofheatresistanceofwheatplantletsbyshorttermheating AT yuekolupaev signalmediatorsatinductionofheatresistanceofwheatplantletsbyshorttermheating AT toyastreb signalmediatorsatinductionofheatresistanceofwheatplantletsbyshorttermheating |