Directed evolution of CRISPR-Cas9 to increase its specificity
Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-05477-x |
| _version_ | 1818683056047783936 |
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| author | Jungjoon K. Lee Euihwan Jeong Joonsun Lee Minhee Jung Eunji Shin Young-hoon Kim Kangin Lee Inyoung Jung Daesik Kim Seokjoong Kim Jin-Soo Kim |
| author_facet | Jungjoon K. Lee Euihwan Jeong Joonsun Lee Minhee Jung Eunji Shin Young-hoon Kim Kangin Lee Inyoung Jung Daesik Kim Seokjoong Kim Jin-Soo Kim |
| author_sort | Jungjoon K. Lee |
| collection | DOAJ |
| description | Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity. |
| first_indexed | 2024-12-17T10:28:40Z |
| format | Article |
| id | doaj.art-12e41efab7774824b3a582ae5751b26d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T10:28:40Z |
| publishDate | 2018-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-12e41efab7774824b3a582ae5751b26d2022-12-21T21:52:35ZengNature PortfolioNature Communications2041-17232018-08-019111010.1038/s41467-018-05477-xDirected evolution of CRISPR-Cas9 to increase its specificityJungjoon K. Lee0Euihwan Jeong1Joonsun Lee2Minhee Jung3Eunji Shin4Young-hoon Kim5Kangin Lee6Inyoung Jung7Daesik Kim8Seokjoong Kim9Jin-Soo Kim10ToolgenCenter for Genome Engineering, Institute for Basic Science (IBS)ToolgenToolgenToolgenToolgenToolgenToolgenDepartment of Chemistry, Seoul National UniversityToolgenCenter for Genome Engineering, Institute for Basic Science (IBS)Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.https://doi.org/10.1038/s41467-018-05477-x |
| spellingShingle | Jungjoon K. Lee Euihwan Jeong Joonsun Lee Minhee Jung Eunji Shin Young-hoon Kim Kangin Lee Inyoung Jung Daesik Kim Seokjoong Kim Jin-Soo Kim Directed evolution of CRISPR-Cas9 to increase its specificity Nature Communications |
| title | Directed evolution of CRISPR-Cas9 to increase its specificity |
| title_full | Directed evolution of CRISPR-Cas9 to increase its specificity |
| title_fullStr | Directed evolution of CRISPR-Cas9 to increase its specificity |
| title_full_unstemmed | Directed evolution of CRISPR-Cas9 to increase its specificity |
| title_short | Directed evolution of CRISPR-Cas9 to increase its specificity |
| title_sort | directed evolution of crispr cas9 to increase its specificity |
| url | https://doi.org/10.1038/s41467-018-05477-x |
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