Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening
Root restriction (RR) has been reported to enhance grape berry quality in diverse aspects of grape life. In this study, RR-induced increases in the main primary metabolites in the grape berry and the expression of their related genes were studied at different developmental stages. Mainly the transcr...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/13/2/281 |
| _version_ | 1797479905025327104 |
|---|---|
| author | Feng Leng Yue Wang Jinping Cao Shiping Wang Di Wu Ling Jiang Xian Li Jinsong Bao Naymul Karim Chongde Sun |
| author_facet | Feng Leng Yue Wang Jinping Cao Shiping Wang Di Wu Ling Jiang Xian Li Jinsong Bao Naymul Karim Chongde Sun |
| author_sort | Feng Leng |
| collection | DOAJ |
| description | Root restriction (RR) has been reported to enhance grape berry quality in diverse aspects of grape life. In this study, RR-induced increases in the main primary metabolites in the grape berry and the expression of their related genes were studied at different developmental stages. Mainly the transcriptomic and metabolomic level were analyzed using ‘Summer Black’ grape berry as a material. The main results were as follows: A total of 11 transcripts involved in the primary metabolic pathways were significantly changed by the RR treatment. Metabolites such as sugars, organic acids, amino acids, starch, pectin, and cellulose were qualitatively and quantitatively analyzed along with their metabolic pathways. Sucrose synthase (<i>VIT_07s0005g00750</i>, <i>VIT_11s0016g00470</i>) and sucrose phosphate synthase (<i>VIT_18s0089g00410</i>) were inferred to play critical roles in the accumulation of starch, sucrose, glucose, and fructose, which was induced by the RR treatment. RR treatment also promoted the malic acid and tartaric acid accumulation in the young berry. In addition, the grape berries after the RR treatment tended to have lower pectin and cellulose content. |
| first_indexed | 2024-03-09T21:52:29Z |
| format | Article |
| id | doaj.art-12e794fc4e614b59b55ea76d900bbefc |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T21:52:29Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-12e794fc4e614b59b55ea76d900bbefc2023-11-23T20:04:25ZengMDPI AGGenes2073-44252022-01-0113228110.3390/genes13020281Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and RipeningFeng Leng0Yue Wang1Jinping Cao2Shiping Wang3Di Wu4Ling Jiang5Xian Li6Jinsong Bao7Naymul Karim8Chongde Sun9Laboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaWujiang Research Institute of Grape, Jinhua 321017, ChinaLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaInstitute of Nuclear Agricultural Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaRoot restriction (RR) has been reported to enhance grape berry quality in diverse aspects of grape life. In this study, RR-induced increases in the main primary metabolites in the grape berry and the expression of their related genes were studied at different developmental stages. Mainly the transcriptomic and metabolomic level were analyzed using ‘Summer Black’ grape berry as a material. The main results were as follows: A total of 11 transcripts involved in the primary metabolic pathways were significantly changed by the RR treatment. Metabolites such as sugars, organic acids, amino acids, starch, pectin, and cellulose were qualitatively and quantitatively analyzed along with their metabolic pathways. Sucrose synthase (<i>VIT_07s0005g00750</i>, <i>VIT_11s0016g00470</i>) and sucrose phosphate synthase (<i>VIT_18s0089g00410</i>) were inferred to play critical roles in the accumulation of starch, sucrose, glucose, and fructose, which was induced by the RR treatment. RR treatment also promoted the malic acid and tartaric acid accumulation in the young berry. In addition, the grape berries after the RR treatment tended to have lower pectin and cellulose content.https://www.mdpi.com/2073-4425/13/2/281root restrictionprimary metabolitesRNA-Seqgrape berry |
| spellingShingle | Feng Leng Yue Wang Jinping Cao Shiping Wang Di Wu Ling Jiang Xian Li Jinsong Bao Naymul Karim Chongde Sun Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening Genes root restriction primary metabolites RNA-Seq grape berry |
| title | Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening |
| title_full | Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening |
| title_fullStr | Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening |
| title_full_unstemmed | Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening |
| title_short | Transcriptomic Analysis of Root Restriction Effects on the Primary Metabolites during Grape Berry Development and Ripening |
| title_sort | transcriptomic analysis of root restriction effects on the primary metabolites during grape berry development and ripening |
| topic | root restriction primary metabolites RNA-Seq grape berry |
| url | https://www.mdpi.com/2073-4425/13/2/281 |
| work_keys_str_mv | AT fengleng transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT yuewang transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT jinpingcao transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT shipingwang transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT diwu transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT lingjiang transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT xianli transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT jinsongbao transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT naymulkarim transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening AT chongdesun transcriptomicanalysisofrootrestrictioneffectsontheprimarymetabolitesduringgrapeberrydevelopmentandripening |