Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids
Background: Keloid, also known as connective tissue hyperplasia, is a benign proliferative disorder with a global distribution. The available therapeutic interventions are steroid injections, surgical removal of keloids, radiotherapy, compression therapy, the application of cryosurgery, and many oth...
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| Format: | Article |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1025546/full |
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| author | Yipeng Dong Yipeng Dong Chuwei Zhang Chuwei Zhang Qingrong Zhang Qingrong Zhang Zihan Li Zihan Li Yixiao Wang Jun Yan Jun Yan Gujie Wu Ling Qiu Ling Qiu Zhihan Zhu Zhihan Zhu Bolin Wang Bolin Wang Haiying Gu Haiying Gu Yi Zhang |
| author_facet | Yipeng Dong Yipeng Dong Chuwei Zhang Chuwei Zhang Qingrong Zhang Qingrong Zhang Zihan Li Zihan Li Yixiao Wang Jun Yan Jun Yan Gujie Wu Ling Qiu Ling Qiu Zhihan Zhu Zhihan Zhu Bolin Wang Bolin Wang Haiying Gu Haiying Gu Yi Zhang |
| author_sort | Yipeng Dong |
| collection | DOAJ |
| description | Background: Keloid, also known as connective tissue hyperplasia, is a benign proliferative disorder with a global distribution. The available therapeutic interventions are steroid injections, surgical removal of keloids, radiotherapy, compression therapy, the application of cryosurgery, and many other methods.Objectives: Existing treatments or approaches for keloids may lead to similar or even larger lesions at the site of keloid excision, leading to a high recurrence rate. Therefore, this study aims at identifying a new gene-based therapy for the treatment of keloids.Methods: An ASPN-siRNA/nanoparticle combination (si-ASPN) and a negative siRNA/nanoparticle complex (NC) was developed on the basis of bioinformatics studies and used in vitro and in vivo experiments.Results: The results showed a strong correlation between the development of keloids and high expression of ASPN protein. With the expression of ASPN protein greatly reduced in keloid fibroblasts and nude mice allografts after treatment with si-ASPN, the collagen and fibroblasts were also uniform, thinner, parallel and regular.Conclusion: All the above experimental results suggest that keloid and ASPN are closely related and both fibroblast growth and metabolism of keloid are inhibited after silencing ASPN. Therefore, ASPN-siRNA delivered via nanoparticles can serve as a novel intervention therapy for the treatment of keloids. |
| first_indexed | 2024-04-12T17:59:02Z |
| format | Article |
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| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T17:59:02Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-12e7dade1f3b4f57bafaffc58ff3b8e42022-12-22T03:22:14ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-10-011010.3389/fbioe.2022.10255461025546Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloidsYipeng Dong0Yipeng Dong1Chuwei Zhang2Chuwei Zhang3Qingrong Zhang4Qingrong Zhang5Zihan Li6Zihan Li7Yixiao Wang8Jun Yan9Jun Yan10Gujie Wu11Ling Qiu12Ling Qiu13Zhihan Zhu14Zhihan Zhu15Bolin Wang16Bolin Wang17Haiying Gu18Haiying Gu19Yi Zhang20Department of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaInstitute of Burn Research, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaMedical School of Nantong University, Nantong, ChinaInstitute of Analytical Chemistry for Life Science, Nantong University, Nantong, ChinaSchool of Public Health, Nantong University, Nantong, ChinaDepartment of Burns and Plastic Surgery, Affiliated Hospital of Nantong University, Nantong, ChinaBackground: Keloid, also known as connective tissue hyperplasia, is a benign proliferative disorder with a global distribution. The available therapeutic interventions are steroid injections, surgical removal of keloids, radiotherapy, compression therapy, the application of cryosurgery, and many other methods.Objectives: Existing treatments or approaches for keloids may lead to similar or even larger lesions at the site of keloid excision, leading to a high recurrence rate. Therefore, this study aims at identifying a new gene-based therapy for the treatment of keloids.Methods: An ASPN-siRNA/nanoparticle combination (si-ASPN) and a negative siRNA/nanoparticle complex (NC) was developed on the basis of bioinformatics studies and used in vitro and in vivo experiments.Results: The results showed a strong correlation between the development of keloids and high expression of ASPN protein. With the expression of ASPN protein greatly reduced in keloid fibroblasts and nude mice allografts after treatment with si-ASPN, the collagen and fibroblasts were also uniform, thinner, parallel and regular.Conclusion: All the above experimental results suggest that keloid and ASPN are closely related and both fibroblast growth and metabolism of keloid are inhibited after silencing ASPN. Therefore, ASPN-siRNA delivered via nanoparticles can serve as a novel intervention therapy for the treatment of keloids.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1025546/fullkeloidasporin genenanoparticlekeloid fibroblastnude mice allograftASPN |
| spellingShingle | Yipeng Dong Yipeng Dong Chuwei Zhang Chuwei Zhang Qingrong Zhang Qingrong Zhang Zihan Li Zihan Li Yixiao Wang Jun Yan Jun Yan Gujie Wu Ling Qiu Ling Qiu Zhihan Zhu Zhihan Zhu Bolin Wang Bolin Wang Haiying Gu Haiying Gu Yi Zhang Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids Frontiers in Bioengineering and Biotechnology keloid asporin gene nanoparticle keloid fibroblast nude mice allograft ASPN |
| title | Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids |
| title_full | Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids |
| title_fullStr | Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids |
| title_full_unstemmed | Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids |
| title_short | Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids |
| title_sort | identification of nanoparticle mediated sirna aspn as a key gene target in the treatment of keloids |
| topic | keloid asporin gene nanoparticle keloid fibroblast nude mice allograft ASPN |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1025546/full |
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