Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants
Halophyte plants are potential resources to deal with the increasing soil salinity determined by climatic change. In this context, the present study aimed to investigate the germplasm conservation of <i>Artemisia caerulescens</i> collected in the San Rossore Estate (Pisa, Italy) through...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/8/1081 |
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| author | Ylenia Pieracci Martina Vento Luisa Pistelli Tiziana Lombardi Laura Pistelli |
| author_facet | Ylenia Pieracci Martina Vento Luisa Pistelli Tiziana Lombardi Laura Pistelli |
| author_sort | Ylenia Pieracci |
| collection | DOAJ |
| description | Halophyte plants are potential resources to deal with the increasing soil salinity determined by climatic change. In this context, the present study aimed to investigate the germplasm conservation of <i>Artemisia caerulescens</i> collected in the San Rossore Estate (Pisa, Italy) through in vitro culture, biochemical properties, and the phytochemical composition of the volatile fraction of both in vitro shoots and different organs of wild plants (leaves, young and ripe inflorescences). The best medium tested for the shoot proliferation was MS, with the addition of 1 μM BA. Total polyphenol content and antioxidant activity were noticeable in both the inflorescences, while leaves and in vitro shoots showed lower amounts. Concerning the phytochemical investigation, the headspaces (HSs) and the essential oils (EOs) were characterized by oxygenated monoterpenes as the main chemical class of compounds in all samples, and with α- and β-thujone as the major constituents. However, the EOs were characterized by noticeable percentages of phenylpropanoids (23.6–28.8%), with brevifolin as the unique compound, which was not detected in the spontaneous volatile emissions of the same parts of the wild plant. Good amounts of EOs were obtained from different organs of the wild plant, comprising between 0.17% and 0.41% of the young and ripe inflorescences, respectively. |
| first_indexed | 2024-03-09T10:29:47Z |
| format | Article |
| id | doaj.art-12e8c95f25a24e938d9dd426e2aada41 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T10:29:47Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-12e8c95f25a24e938d9dd426e2aada412023-12-01T21:19:47ZengMDPI AGPlants2223-77472022-04-01118108110.3390/plants11081081Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild PlantsYlenia Pieracci0Martina Vento1Luisa Pistelli2Tiziana Lombardi3Laura Pistelli4Dipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, ItalyDipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, ItalyDipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, ItalyDipartimento di Scienze Agrarie, Alimentari e Agro-Alimentari, Università di Pisa, Via del Borghetto 80, 56124 Pisa, ItalyCentro Interdipartimentale di Ricerca Nutraceutica e Alimentazione per la Salute ‘NUTRAFOOD’, Università di Pisa, Via del Borghetto 80, 56124 Pisa, ItalyHalophyte plants are potential resources to deal with the increasing soil salinity determined by climatic change. In this context, the present study aimed to investigate the germplasm conservation of <i>Artemisia caerulescens</i> collected in the San Rossore Estate (Pisa, Italy) through in vitro culture, biochemical properties, and the phytochemical composition of the volatile fraction of both in vitro shoots and different organs of wild plants (leaves, young and ripe inflorescences). The best medium tested for the shoot proliferation was MS, with the addition of 1 μM BA. Total polyphenol content and antioxidant activity were noticeable in both the inflorescences, while leaves and in vitro shoots showed lower amounts. Concerning the phytochemical investigation, the headspaces (HSs) and the essential oils (EOs) were characterized by oxygenated monoterpenes as the main chemical class of compounds in all samples, and with α- and β-thujone as the major constituents. However, the EOs were characterized by noticeable percentages of phenylpropanoids (23.6–28.8%), with brevifolin as the unique compound, which was not detected in the spontaneous volatile emissions of the same parts of the wild plant. Good amounts of EOs were obtained from different organs of the wild plant, comprising between 0.17% and 0.41% of the young and ripe inflorescences, respectively.https://www.mdpi.com/2223-7747/11/8/1081shoot proliferationpolyphenolsantioxidant activityessential oilsHS-SPMEGC-MS |
| spellingShingle | Ylenia Pieracci Martina Vento Luisa Pistelli Tiziana Lombardi Laura Pistelli Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants Plants shoot proliferation polyphenols antioxidant activity essential oils HS-SPME GC-MS |
| title | Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants |
| title_full | Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants |
| title_fullStr | Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants |
| title_full_unstemmed | Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants |
| title_short | Halophyte <i>Artemisia caerulescens</i> L.: Metabolites from In Vitro Shoots and Wild Plants |
| title_sort | halophyte i artemisia caerulescens i l metabolites from in vitro shoots and wild plants |
| topic | shoot proliferation polyphenols antioxidant activity essential oils HS-SPME GC-MS |
| url | https://www.mdpi.com/2223-7747/11/8/1081 |
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