Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile
The COVID-19 pandemic continues to be a concern and keeps global health authorities on alert. The RT-PCR technique has been the gold-standard assay for detecting the SARS-CoV-2 virus. However, rapid antigen tests (RATs) have been widely used to increase the number of tests faster and more efficientl...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Public Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2022.976875/full |
| _version_ | 1811251975615938560 |
|---|---|
| author | Carlos Barrera-Avalos Javier Mena Roberto Luraschi Patricio Rojas Carlos Mateluna-Flores Eva Vallejos-Vidal Mónica Imarai Mónica Imarai Ana María Sandino Ana María Sandino Daniel Valdés Daniel Valdés Rodrigo Vera Iván Hernández Felipe E. Reyes-López Claudio Acuña-Castillo Claudio Acuña-Castillo |
| author_facet | Carlos Barrera-Avalos Javier Mena Roberto Luraschi Patricio Rojas Carlos Mateluna-Flores Eva Vallejos-Vidal Mónica Imarai Mónica Imarai Ana María Sandino Ana María Sandino Daniel Valdés Daniel Valdés Rodrigo Vera Iván Hernández Felipe E. Reyes-López Claudio Acuña-Castillo Claudio Acuña-Castillo |
| author_sort | Carlos Barrera-Avalos |
| collection | DOAJ |
| description | The COVID-19 pandemic continues to be a concern and keeps global health authorities on alert. The RT-PCR technique has been the gold-standard assay for detecting the SARS-CoV-2 virus. However, rapid antigen tests (RATs) have been widely used to increase the number of tests faster and more efficiently in the population. Nevertheless, the appearance of new viral variants, with genomic mutations associated with greater contagiousness and immune evasion, highlights the need to evaluate the sensitivity of these RATs. This report evaluates the sensitivity of SD Biosensor-Roche, Panbio™, and Clinitest® RATs widely used in Santiago de Chile in the detection of the Omicron variant from Nasopharyngeal samples (NPSs), the most predominant SARS-CoV-2 variant in Chile and the world. SD Biosensor-Roche shows a detection sensitivity of 95.7% in the viral amplification range of 20 ≤ Cq < 25, while Panbio™ and Clinitest® show 100% and 91.3%, respectively. In the viral amplification ranges of 25 ≤ Cq < 30, the detection sensitivity decreased to 28% for SD Biosensor-Roche, 32% for Panbio™, and 72% for Clinitest®. This study indicates that the tested RATs have high sensitivity in detecting the Omicron variant of concern (VOC) at high viral loads. By contrast, its sensitivity decreases at low viral loads. Therefore, it is suggested to limit the use of RATs as an active search method, considering that infections in patients are increasingly associated with lower viral loads of SARS-CoV-2. These antecedents could prevent contagion outbreaks and reduce the underestimation of the current Omicron variant circulation at the local level. |
| first_indexed | 2024-04-12T16:28:01Z |
| format | Article |
| id | doaj.art-12e9607aa6de4e4d85b5c9681f704400 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-04-12T16:28:01Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-12e9607aa6de4e4d85b5c9681f7044002022-12-22T03:25:18ZengFrontiers Media S.A.Frontiers in Public Health2296-25652022-10-011010.3389/fpubh.2022.976875976875Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of ChileCarlos Barrera-Avalos0Javier Mena1Roberto Luraschi2Patricio Rojas3Carlos Mateluna-Flores4Eva Vallejos-Vidal5Mónica Imarai6Mónica Imarai7Ana María Sandino8Ana María Sandino9Daniel Valdés10Daniel Valdés11Rodrigo Vera12Iván Hernández13Felipe E. Reyes-López14Claudio Acuña-Castillo15Claudio Acuña-Castillo16Centro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileHospital de Urgencia Asistencia Pública (HUAP), Santiago, ChileHospital de Urgencia Asistencia Pública (HUAP), Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileThe COVID-19 pandemic continues to be a concern and keeps global health authorities on alert. The RT-PCR technique has been the gold-standard assay for detecting the SARS-CoV-2 virus. However, rapid antigen tests (RATs) have been widely used to increase the number of tests faster and more efficiently in the population. Nevertheless, the appearance of new viral variants, with genomic mutations associated with greater contagiousness and immune evasion, highlights the need to evaluate the sensitivity of these RATs. This report evaluates the sensitivity of SD Biosensor-Roche, Panbio™, and Clinitest® RATs widely used in Santiago de Chile in the detection of the Omicron variant from Nasopharyngeal samples (NPSs), the most predominant SARS-CoV-2 variant in Chile and the world. SD Biosensor-Roche shows a detection sensitivity of 95.7% in the viral amplification range of 20 ≤ Cq < 25, while Panbio™ and Clinitest® show 100% and 91.3%, respectively. In the viral amplification ranges of 25 ≤ Cq < 30, the detection sensitivity decreased to 28% for SD Biosensor-Roche, 32% for Panbio™, and 72% for Clinitest®. This study indicates that the tested RATs have high sensitivity in detecting the Omicron variant of concern (VOC) at high viral loads. By contrast, its sensitivity decreases at low viral loads. Therefore, it is suggested to limit the use of RATs as an active search method, considering that infections in patients are increasingly associated with lower viral loads of SARS-CoV-2. These antecedents could prevent contagion outbreaks and reduce the underestimation of the current Omicron variant circulation at the local level.https://www.frontiersin.org/articles/10.3389/fpubh.2022.976875/fullrapid antigen testSARS-CoV-2Omicron detectionCOVID-19RT-qPCRdetection sensitivity |
| spellingShingle | Carlos Barrera-Avalos Javier Mena Roberto Luraschi Patricio Rojas Carlos Mateluna-Flores Eva Vallejos-Vidal Mónica Imarai Mónica Imarai Ana María Sandino Ana María Sandino Daniel Valdés Daniel Valdés Rodrigo Vera Iván Hernández Felipe E. Reyes-López Claudio Acuña-Castillo Claudio Acuña-Castillo Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile Frontiers in Public Health rapid antigen test SARS-CoV-2 Omicron detection COVID-19 RT-qPCR detection sensitivity |
| title | Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile |
| title_full | Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile |
| title_fullStr | Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile |
| title_full_unstemmed | Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile |
| title_short | Sensitivity analysis of rapid antigen tests for the Omicron SARS-CoV-2 variant detection from nasopharyngeal swab samples collected in Santiago of Chile |
| title_sort | sensitivity analysis of rapid antigen tests for the omicron sars cov 2 variant detection from nasopharyngeal swab samples collected in santiago of chile |
| topic | rapid antigen test SARS-CoV-2 Omicron detection COVID-19 RT-qPCR detection sensitivity |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2022.976875/full |
| work_keys_str_mv | AT carlosbarreraavalos sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT javiermena sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT robertoluraschi sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT patriciorojas sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT carlosmatelunaflores sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT evavallejosvidal sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT monicaimarai sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT monicaimarai sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT anamariasandino sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT anamariasandino sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT danielvaldes sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT danielvaldes sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT rodrigovera sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT ivanhernandez sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT felipeereyeslopez sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT claudioacunacastillo sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile AT claudioacunacastillo sensitivityanalysisofrapidantigentestsfortheomicronsarscov2variantdetectionfromnasopharyngealswabsamplescollectedinsantiagoofchile |