Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis
The response of different plant growth regulators on callus induction and regeneration on three sugarcane genotypes (YT-53, CP-77-400, and NSG-59) was evaluated. Different concentrations of 2,4-D alone and in combination with other plant growth regulators (Kinetin and BAP) were used for callus induc...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/12/11/1812 |
| _version_ | 1797469455500967936 |
|---|---|
| author | Yasmeen Saleem Muhammad Zaka Emad Aamir Ali Shagufta Naz |
| author_facet | Yasmeen Saleem Muhammad Zaka Emad Aamir Ali Shagufta Naz |
| author_sort | Yasmeen Saleem |
| collection | DOAJ |
| description | The response of different plant growth regulators on callus induction and regeneration on three sugarcane genotypes (YT-53, CP-77-400, and NSG-59) was evaluated. Different concentrations of 2,4-D alone and in combination with other plant growth regulators (Kinetin and BAP) were used for callus induction. Kinetin along with IBA, BAP and NAA were analyzed with respect to shoot induction, while NAA and IBA were used for root induction. The best callus response in terms of number of days, callus fresh weight, and frequency in YT-53 was observed on MS media provided with 2,4-D (3 mg L<sup>−1</sup>) + Kinetin (0.5 mg L<sup>−1</sup>), while in NSG-59 the best response was on MS+2,4-D (4 mg L<sup>−1</sup>) + Kinetin (0.5 mg L<sup>−1</sup>), and in CP-77400, MS+2,4-D (5 mg L<sup>−1</sup>). For shoot induction, 2 mg L<sup>−1</sup> Kinetin was found to be the best for YT-53 and NSG-59, while 1 mg L<sup>−1</sup> BAP was found to be the best for CP-77-400 in terms of number of days, shoot numbers, and shoot length. The best media for root induction in terms of number of days, root numbers, and root length was 1 mg L<sup>−1</sup> NAA + 1 mg L<sup>−1</sup> IBA for YT-53, while this was 3 mg L<sup>−1</sup> NAA for NSG-59. The highest root frequency and maximum root length in the minimum number of days was observed in CP-77-400 on MS media provided with 2 mg L<sup>−1</sup> NAA. |
| first_indexed | 2024-03-09T19:21:39Z |
| format | Article |
| id | doaj.art-12ec12d717224603bab64e8f8ef2347a |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T19:21:39Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-12ec12d717224603bab64e8f8ef2347a2023-11-24T03:17:20ZengMDPI AGAgriculture2077-04722022-10-011211181210.3390/agriculture12111812Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by CallogenesisYasmeen Saleem0Muhammad Zaka Emad1Aamir Ali2Shagufta Naz3Department of Botany, University of Sargodha, Sargodha 40162, PakistanDepartment of Mining Engineering, University of Engineering and Technology, Lahore 54890, PakistanDepartment of Botany, University of Sargodha, Sargodha 40162, PakistanDepartment of Biotechnology, Lahore College for Women University, Lahore 54000, PakistanThe response of different plant growth regulators on callus induction and regeneration on three sugarcane genotypes (YT-53, CP-77-400, and NSG-59) was evaluated. Different concentrations of 2,4-D alone and in combination with other plant growth regulators (Kinetin and BAP) were used for callus induction. Kinetin along with IBA, BAP and NAA were analyzed with respect to shoot induction, while NAA and IBA were used for root induction. The best callus response in terms of number of days, callus fresh weight, and frequency in YT-53 was observed on MS media provided with 2,4-D (3 mg L<sup>−1</sup>) + Kinetin (0.5 mg L<sup>−1</sup>), while in NSG-59 the best response was on MS+2,4-D (4 mg L<sup>−1</sup>) + Kinetin (0.5 mg L<sup>−1</sup>), and in CP-77400, MS+2,4-D (5 mg L<sup>−1</sup>). For shoot induction, 2 mg L<sup>−1</sup> Kinetin was found to be the best for YT-53 and NSG-59, while 1 mg L<sup>−1</sup> BAP was found to be the best for CP-77-400 in terms of number of days, shoot numbers, and shoot length. The best media for root induction in terms of number of days, root numbers, and root length was 1 mg L<sup>−1</sup> NAA + 1 mg L<sup>−1</sup> IBA for YT-53, while this was 3 mg L<sup>−1</sup> NAA for NSG-59. The highest root frequency and maximum root length in the minimum number of days was observed in CP-77-400 on MS media provided with 2 mg L<sup>−1</sup> NAA.https://www.mdpi.com/2077-0472/12/11/1812callus inductionKinetinregenerationroot inductiontissue culture |
| spellingShingle | Yasmeen Saleem Muhammad Zaka Emad Aamir Ali Shagufta Naz Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis Agriculture callus induction Kinetin regeneration root induction tissue culture |
| title | Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis |
| title_full | Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis |
| title_fullStr | Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis |
| title_full_unstemmed | Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis |
| title_short | Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (<i>Saccharum officinarum</i> L.) by Callogenesis |
| title_sort | synergetic effect of different plant growth regulators on micropropagation of sugarcane i saccharum officinarum i l by callogenesis |
| topic | callus induction Kinetin regeneration root induction tissue culture |
| url | https://www.mdpi.com/2077-0472/12/11/1812 |
| work_keys_str_mv | AT yasmeensaleem synergeticeffectofdifferentplantgrowthregulatorsonmicropropagationofsugarcaneisaccharumofficinarumilbycallogenesis AT muhammadzakaemad synergeticeffectofdifferentplantgrowthregulatorsonmicropropagationofsugarcaneisaccharumofficinarumilbycallogenesis AT aamirali synergeticeffectofdifferentplantgrowthregulatorsonmicropropagationofsugarcaneisaccharumofficinarumilbycallogenesis AT shaguftanaz synergeticeffectofdifferentplantgrowthregulatorsonmicropropagationofsugarcaneisaccharumofficinarumilbycallogenesis |