GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae
Phytophthora root and stem rot, a destructive disease of soybean [Glycine max (L.) Merr.], is caused by the oomycete Phytophthora sojae. However, how the disease resistance mechanisms of soybean respond to P. sojae infection remains unclear. Previously, we showed that GmWRKY31, which interacts with...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00996/full |
| _version_ | 1811292885168947200 |
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| author | Le Wang Huiyu Wang Shengfu He Fanshan Meng Chuanzhong Zhang Sujie Fan Sujie Fan Junjiang Wu Shuzhen Zhang Pengfei Xu |
| author_facet | Le Wang Huiyu Wang Shengfu He Fanshan Meng Chuanzhong Zhang Sujie Fan Sujie Fan Junjiang Wu Shuzhen Zhang Pengfei Xu |
| author_sort | Le Wang |
| collection | DOAJ |
| description | Phytophthora root and stem rot, a destructive disease of soybean [Glycine max (L.) Merr.], is caused by the oomycete Phytophthora sojae. However, how the disease resistance mechanisms of soybean respond to P. sojae infection remains unclear. Previously, we showed that GmWRKY31, which interacts with a sucrose non-fermenting-1(SNF1)-related protein kinase (SnRK), enhances resistance to P. sojae in soybean. Here, we report that the membrane-localized SnRK GmSnRK1.1 is involved in the soybean host response to P. sojae. The overexpression of GmSnRK1.1 (GmSnRK1.1-OE) increased soybean resistance to P. sojae, and the RNA interference (RNAi)-mediated silencing of GmSnRK1.1 (GmSnRK1.1-R) reduced resistance to P. sojae. Moreover, the activities and transcript levels of the antioxidant enzymes superoxide dismutase and peroxidase were markedly higher in the GmSnRK1.1-OE transgenic soybean plants than in the wild type (WT), but were reduced in the GmSnRK1.1-R plants. Several isoflavonoid phytoalexins related genes GmPAL, GmIFR, Gm4CL and GmCHS were significantly higher in “Suinong 10” and GmSnRK1.1-OE lines than these in “Dongnong 50,” and were significantly lower in GmSnRK1.1-R lines. In addition, the accumulation of salicylic acid (SA) and the expression level of the SA biosynthesis-related gene were significantly higher in the GmSnRK1.1-OE plants than in the WT and GmSnRK1.1-R plants, moreover, SA biosynthesis inhibitor treated GmSnRK1.1-R lines plants displayed clearly increased pathogen biomass compared with H2O-treated plants after 24 h post-inoculation. These results showed that GmSnRK1.1 positively regulates soybean resistance to P. sojae, potentially functioning via effects on the expression of SA-related genes and increased accumulation of SA. |
| first_indexed | 2024-04-13T04:53:08Z |
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| id | doaj.art-12ef58d15c8c4242a5acf6996c0bb1cc |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T04:53:08Z |
| publishDate | 2019-08-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-12ef58d15c8c4242a5acf6996c0bb1cc2022-12-22T03:01:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-08-011010.3389/fpls.2019.00996460676GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojaeLe Wang0Huiyu Wang1Shengfu He2Fanshan Meng3Chuanzhong Zhang4Sujie Fan5Sujie Fan6Junjiang Wu7Shuzhen Zhang8Pengfei Xu9Soybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaCollege of Agronomy, Plant Biotechnology Center, Jilin Agricultural University, Changchun, ChinaSoybean Research Institute of Heilongjiang Academy of Agricultural Sciences, Key Laboratory of Soybean Cultivation of Ministry of Agriculture P. R. China, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaSoybean Research Institute/Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, ChinaPhytophthora root and stem rot, a destructive disease of soybean [Glycine max (L.) Merr.], is caused by the oomycete Phytophthora sojae. However, how the disease resistance mechanisms of soybean respond to P. sojae infection remains unclear. Previously, we showed that GmWRKY31, which interacts with a sucrose non-fermenting-1(SNF1)-related protein kinase (SnRK), enhances resistance to P. sojae in soybean. Here, we report that the membrane-localized SnRK GmSnRK1.1 is involved in the soybean host response to P. sojae. The overexpression of GmSnRK1.1 (GmSnRK1.1-OE) increased soybean resistance to P. sojae, and the RNA interference (RNAi)-mediated silencing of GmSnRK1.1 (GmSnRK1.1-R) reduced resistance to P. sojae. Moreover, the activities and transcript levels of the antioxidant enzymes superoxide dismutase and peroxidase were markedly higher in the GmSnRK1.1-OE transgenic soybean plants than in the wild type (WT), but were reduced in the GmSnRK1.1-R plants. Several isoflavonoid phytoalexins related genes GmPAL, GmIFR, Gm4CL and GmCHS were significantly higher in “Suinong 10” and GmSnRK1.1-OE lines than these in “Dongnong 50,” and were significantly lower in GmSnRK1.1-R lines. In addition, the accumulation of salicylic acid (SA) and the expression level of the SA biosynthesis-related gene were significantly higher in the GmSnRK1.1-OE plants than in the WT and GmSnRK1.1-R plants, moreover, SA biosynthesis inhibitor treated GmSnRK1.1-R lines plants displayed clearly increased pathogen biomass compared with H2O-treated plants after 24 h post-inoculation. These results showed that GmSnRK1.1 positively regulates soybean resistance to P. sojae, potentially functioning via effects on the expression of SA-related genes and increased accumulation of SA.https://www.frontiersin.org/article/10.3389/fpls.2019.00996/fullGlycine maxGmSnRK1.1enzymatic antioxidantssalicylic acidPhytophthora sojae |
| spellingShingle | Le Wang Huiyu Wang Shengfu He Fanshan Meng Chuanzhong Zhang Sujie Fan Sujie Fan Junjiang Wu Shuzhen Zhang Pengfei Xu GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae Frontiers in Plant Science Glycine max GmSnRK1.1 enzymatic antioxidants salicylic acid Phytophthora sojae |
| title | GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae |
| title_full | GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae |
| title_fullStr | GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae |
| title_full_unstemmed | GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae |
| title_short | GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae |
| title_sort | gmsnrk1 1 a sucrose non fermenting 1 snf1 related protein kinase promotes soybean resistance to phytophthora sojae |
| topic | Glycine max GmSnRK1.1 enzymatic antioxidants salicylic acid Phytophthora sojae |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00996/full |
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