A highly efficient scheme for library preparation from single-stranded DNA

Abstract Although methods for sequencing library preparation from double-stranded DNA are well established, those from single-stranded DNA (ssDNA) have not been well studied. Further, the existing methods have limitations in efficiency and yield. Therefore, we developed a highly efficient procedure...

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Bibliographic Details
Main Authors: Fumihito Miura, Hideaki Kanzawa-Kiriyama, Osamu Hisano, Miki Miura, Yukiko Shibata, Noboru Adachi, Tsuneo Kakuda, Ken-ichi Shinoda, Takashi Ito
Format: Article
Language:English
Published: Nature Portfolio 2023-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-40890-3