Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level
Gestational diabetes mellitus (GDM) is associated with an increased risk of adverse pregnancy outcomes. Increasing evidence shows that placentation defects may play important roles in GDM. However, our understanding of the human placenta remains limited. In this study, we generated a comprehensive t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.679582/full |
| _version_ | 1819298595559440384 |
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| author | Yuqi Yang Fang Guo Yue Peng Rong Chen Wenbo Zhou Huihui Wang Jun OuYang Bin Yu Zhengfeng Xu |
| author_facet | Yuqi Yang Fang Guo Yue Peng Rong Chen Wenbo Zhou Huihui Wang Jun OuYang Bin Yu Zhengfeng Xu |
| author_sort | Yuqi Yang |
| collection | DOAJ |
| description | Gestational diabetes mellitus (GDM) is associated with an increased risk of adverse pregnancy outcomes. Increasing evidence shows that placentation defects may play important roles in GDM. However, our understanding of the human placenta remains limited. In this study, we generated a comprehensive transcriptomic profile of cellular signatures and transcriptomes in the human placenta in GDM using single-cell RNA sequencing (scRNA-seq), constructed a comprehensive cell atlas, and identified cell subtypes and subtype-specific marker genes. In addition, we investigated the placental cellular function and intercellular interactions in GDM. These findings help to elucidate the molecular mechanisms of GDM, and may facilitate the development of new approaches to GDM treatment and prevention. |
| first_indexed | 2024-12-24T05:32:24Z |
| format | Article |
| id | doaj.art-12f3ee8765214ed08175c4ca42293384 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-24T05:32:24Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-12f3ee8765214ed08175c4ca422933842022-12-21T17:13:07ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-05-011210.3389/fendo.2021.679582679582Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell LevelYuqi Yang0Fang Guo1Yue Peng2Rong Chen3Wenbo Zhou4Huihui Wang5Jun OuYang6Bin Yu7Zhengfeng Xu8Changzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaChangzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaChangzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaInternational Genome Center, Jiangsu University, Zhenjiang, ChinaChangzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaChangzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaChangzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaChangzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, ChinaWomen’s Hospital of Nanjing Medical University, Nanjing, ChinaGestational diabetes mellitus (GDM) is associated with an increased risk of adverse pregnancy outcomes. Increasing evidence shows that placentation defects may play important roles in GDM. However, our understanding of the human placenta remains limited. In this study, we generated a comprehensive transcriptomic profile of cellular signatures and transcriptomes in the human placenta in GDM using single-cell RNA sequencing (scRNA-seq), constructed a comprehensive cell atlas, and identified cell subtypes and subtype-specific marker genes. In addition, we investigated the placental cellular function and intercellular interactions in GDM. These findings help to elucidate the molecular mechanisms of GDM, and may facilitate the development of new approaches to GDM treatment and prevention.https://www.frontiersin.org/articles/10.3389/fendo.2021.679582/fullgestational diabetes mellitusplacentasingle-cell RNA sequencingcellular signaturestranscriptomes |
| spellingShingle | Yuqi Yang Fang Guo Yue Peng Rong Chen Wenbo Zhou Huihui Wang Jun OuYang Bin Yu Zhengfeng Xu Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level Frontiers in Endocrinology gestational diabetes mellitus placenta single-cell RNA sequencing cellular signatures transcriptomes |
| title | Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level |
| title_full | Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level |
| title_fullStr | Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level |
| title_full_unstemmed | Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level |
| title_short | Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level |
| title_sort | transcriptomic profiling of human placenta in gestational diabetes mellitus at the single cell level |
| topic | gestational diabetes mellitus placenta single-cell RNA sequencing cellular signatures transcriptomes |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2021.679582/full |
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