Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field
Abstract Enzymatic degradation of emerging contaminants has gained great interest for the past few years. However, free enzyme often incurs high costs in practice. The immobilized laccase on the polyethylenimine (PEI)‐functionalized magnetic nanoparticles (Fe3O4–NH2–PEI–laccase) was fabricated to ef...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2021-06-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202100009 |
| _version_ | 1818865381632114688 |
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| author | Ting‐Ting Xia Mei Feng Chun‐Lei Liu Chun‐Zhao Liu Chen Guo |
| author_facet | Ting‐Ting Xia Mei Feng Chun‐Lei Liu Chun‐Zhao Liu Chen Guo |
| author_sort | Ting‐Ting Xia |
| collection | DOAJ |
| description | Abstract Enzymatic degradation of emerging contaminants has gained great interest for the past few years. However, free enzyme often incurs high costs in practice. The immobilized laccase on the polyethylenimine (PEI)‐functionalized magnetic nanoparticles (Fe3O4–NH2–PEI–laccase) was fabricated to efficiently degrade phenolic compounds continuously in a newly fixed bed reactor under a high‐gradient magnetic field. The degradation rate of continuous treatment in the bed after 18 h was 2.38 times as high as that of batch treatment after six successive operations with the same treatment duration. Under the optimal conditions of volume fraction of nickel wires mesh, flow rate of phenol solution, phenol concentration, and Fe3O4–NH2–PEI–laccase amount, the degradation rate of phenol kept over 70.30% in 48 h continuous treatment. The fixed bed reactor filled with Fe3O4–NH2–PEI–laccase provided a promising avenue for the continuous biodegradation of phenolic compounds for industrial wastewater in practice. |
| first_indexed | 2024-12-19T10:46:39Z |
| format | Article |
| id | doaj.art-12f47a83b66f4eca8b451d4d8ebe5cea |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-12-19T10:46:39Z |
| publishDate | 2021-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-12f47a83b66f4eca8b451d4d8ebe5cea2022-12-21T20:25:15ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632021-06-0121637438110.1002/elsc.202100009Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic fieldTing‐Ting Xia0Mei Feng1Chun‐Lei Liu2Chun‐Zhao Liu3Chen Guo4State Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaState Key Laboratory of Bio‐fibers and Eco‐textiles Institute of Biochemical Engineering Affiliated Qingdao Central Hospital College of Materials Science and Engineering Qingdao University Qingdao P. R. ChinaState Key Laboratory of Bio‐fibers and Eco‐textiles Institute of Biochemical Engineering Affiliated Qingdao Central Hospital College of Materials Science and Engineering Qingdao University Qingdao P. R. ChinaState Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaState Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaAbstract Enzymatic degradation of emerging contaminants has gained great interest for the past few years. However, free enzyme often incurs high costs in practice. The immobilized laccase on the polyethylenimine (PEI)‐functionalized magnetic nanoparticles (Fe3O4–NH2–PEI–laccase) was fabricated to efficiently degrade phenolic compounds continuously in a newly fixed bed reactor under a high‐gradient magnetic field. The degradation rate of continuous treatment in the bed after 18 h was 2.38 times as high as that of batch treatment after six successive operations with the same treatment duration. Under the optimal conditions of volume fraction of nickel wires mesh, flow rate of phenol solution, phenol concentration, and Fe3O4–NH2–PEI–laccase amount, the degradation rate of phenol kept over 70.30% in 48 h continuous treatment. The fixed bed reactor filled with Fe3O4–NH2–PEI–laccase provided a promising avenue for the continuous biodegradation of phenolic compounds for industrial wastewater in practice.https://doi.org/10.1002/elsc.202100009fixed bed reactorhigh‐gradient magnetic fieldmagnetic immobilized laccasephenol degradation |
| spellingShingle | Ting‐Ting Xia Mei Feng Chun‐Lei Liu Chun‐Zhao Liu Chen Guo Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field Engineering in Life Sciences fixed bed reactor high‐gradient magnetic field magnetic immobilized laccase phenol degradation |
| title | Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field |
| title_full | Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field |
| title_fullStr | Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field |
| title_full_unstemmed | Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field |
| title_short | Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field |
| title_sort | efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high gradient magnetic field |
| topic | fixed bed reactor high‐gradient magnetic field magnetic immobilized laccase phenol degradation |
| url | https://doi.org/10.1002/elsc.202100009 |
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