Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus
Lumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV)...
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| Format: | Article |
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MDPI AG
2024-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/7/1676 |
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| author | Samr Kassem Mervat E. Hamdy Karim M. Selim Dalia M. A. Elmasry Momtaz A. Shahein Dalia M. El-Husseini |
| author_facet | Samr Kassem Mervat E. Hamdy Karim M. Selim Dalia M. A. Elmasry Momtaz A. Shahein Dalia M. El-Husseini |
| author_sort | Samr Kassem |
| collection | DOAJ |
| description | Lumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV) virus. Hence, the LSDV traditional diagnostic tools faced many limitations regarding sensitivity, specificity, and cross-reactivity. Herein, we fabricated a paper-based turn-on fluorescent Molecularly Imprinted Polymer (MIP) sensor for the rapid detection of LSDV. The LSDV-MIPs sensor showed strong fluorescent intensity signal enhancement in response to the presence of the virus within minutes. Our sensor showed a limit of detection of 10<sup>1</sup> log<sub>10</sub> TCID50/mL. Moreover, it showed significantly higher specificity to LSDV relative to other viruses, especially SPV. To our knowledge, this is the first record of a paper-based rapid detection test for LSDV depending on fluorescent turn-on behavior. |
| first_indexed | 2024-04-24T10:37:35Z |
| format | Article |
| id | doaj.art-12f5586c560443ffaa1a6b2a57b39e5f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T10:37:35Z |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-12f5586c560443ffaa1a6b2a57b39e5f2024-04-12T13:23:44ZengMDPI AGMolecules1420-30492024-04-01297167610.3390/molecules29071676Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease VirusSamr Kassem0Mervat E. Hamdy1Karim M. Selim2Dalia M. A. Elmasry3Momtaz A. Shahein4Dalia M. El-Husseini5Nanomaterials Research and Synthesis Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, EgyptGenome Research Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, EgyptReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, EgyptNanomaterials Research and Synthesis Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, EgyptVirology Research Department, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, EgyptNanomaterials Research and Synthesis Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, EgyptLumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV) virus. Hence, the LSDV traditional diagnostic tools faced many limitations regarding sensitivity, specificity, and cross-reactivity. Herein, we fabricated a paper-based turn-on fluorescent Molecularly Imprinted Polymer (MIP) sensor for the rapid detection of LSDV. The LSDV-MIPs sensor showed strong fluorescent intensity signal enhancement in response to the presence of the virus within minutes. Our sensor showed a limit of detection of 10<sup>1</sup> log<sub>10</sub> TCID50/mL. Moreover, it showed significantly higher specificity to LSDV relative to other viruses, especially SPV. To our knowledge, this is the first record of a paper-based rapid detection test for LSDV depending on fluorescent turn-on behavior.https://www.mdpi.com/1420-3049/29/7/1676LSDVpaper-basedfluorescentMIPsbiosensor |
| spellingShingle | Samr Kassem Mervat E. Hamdy Karim M. Selim Dalia M. A. Elmasry Momtaz A. Shahein Dalia M. El-Husseini Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus Molecules LSDV paper-based fluorescent MIPs biosensor |
| title | Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus |
| title_full | Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus |
| title_fullStr | Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus |
| title_full_unstemmed | Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus |
| title_short | Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus |
| title_sort | development of paper based fluorescent molecularly imprinted polymer sensor for rapid detection of lumpy skin disease virus |
| topic | LSDV paper-based fluorescent MIPs biosensor |
| url | https://www.mdpi.com/1420-3049/29/7/1676 |
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