Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection
The association of single nucleotide polymorphisms (SNPs) in the interferon (IFN)-γ gene ( IFNG ) with different types of retinal scar lesions presumably caused by toxoplasmosis were investigated in a cross-sectional population-based genetic study. Ten SNPs were investigated and after Bonferroni cor...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100099&lng=en&tlng=en |
| _version_ | 1797715760155459584 |
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| author | Ricardo Guerra Peixe Marcela Santana Bastos Boechat Alba Lucinia Peixoto Rangel Rhonia Franca Gomes Rosa Maria Luiza Petzl-Erler Lilian MG Bahia-Oliveira |
| author_facet | Ricardo Guerra Peixe Marcela Santana Bastos Boechat Alba Lucinia Peixoto Rangel Rhonia Franca Gomes Rosa Maria Luiza Petzl-Erler Lilian MG Bahia-Oliveira |
| author_sort | Ricardo Guerra Peixe |
| collection | DOAJ |
| description | The association of single nucleotide polymorphisms (SNPs) in the interferon (IFN)-γ gene ( IFNG ) with different types of retinal scar lesions presumably caused by toxoplasmosis were investigated in a cross-sectional population-based genetic study. Ten SNPs were investigated and after Bonferroni correction, only the associations between SNPs rs2069718 and rs3181035 with retinal/retinochoroidal scar lesions type A (most severe scar lesions) and C (least severe scar lesions), respectively, remained significant. The associations of two different IFNG SNPs with two different types of retinal lesions attributable to toxoplasmosis support the hypothesis that different inflammatory mechanisms underlie the development of these lesions. The in vitro analysis of IFN-γ secretion by peripheral blood mononuclear cells stimulated with Toxoplasma gondii antigens was also investigated. The association between SNP rs2069718 and type A scar lesions revealed that differential IFN-γ levels are correlated with distinct genotypes. However, no correlation was observed with IFN-γ secretion levels and the SNP rs3181035 , which was significantly associated with type C scar lesions. Our findings strongly suggest that immunogenetic studies of individuals with congenital or postnatally acquired infection are needed to better understand the role of IFN-γ and its polymorphisms in the pathogenesis of ocular toxoplasmosis. |
| first_indexed | 2024-03-12T08:12:30Z |
| format | Article |
| id | doaj.art-12f5e043027d4da2a509f35370723673 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:12:30Z |
| publishDate | 2014-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-12f5e043027d4da2a509f353707236732023-09-02T19:06:34ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-02-0110919910710.1590/0074-0276140539S0074-02762014000100099Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infectionRicardo Guerra PeixeMarcela Santana Bastos BoechatAlba Lucinia Peixoto RangelRhonia Franca Gomes RosaMaria Luiza Petzl-ErlerLilian MG Bahia-OliveiraThe association of single nucleotide polymorphisms (SNPs) in the interferon (IFN)-γ gene ( IFNG ) with different types of retinal scar lesions presumably caused by toxoplasmosis were investigated in a cross-sectional population-based genetic study. Ten SNPs were investigated and after Bonferroni correction, only the associations between SNPs rs2069718 and rs3181035 with retinal/retinochoroidal scar lesions type A (most severe scar lesions) and C (least severe scar lesions), respectively, remained significant. The associations of two different IFNG SNPs with two different types of retinal lesions attributable to toxoplasmosis support the hypothesis that different inflammatory mechanisms underlie the development of these lesions. The in vitro analysis of IFN-γ secretion by peripheral blood mononuclear cells stimulated with Toxoplasma gondii antigens was also investigated. The association between SNP rs2069718 and type A scar lesions revealed that differential IFN-γ levels are correlated with distinct genotypes. However, no correlation was observed with IFN-γ secretion levels and the SNP rs3181035 , which was significantly associated with type C scar lesions. Our findings strongly suggest that immunogenetic studies of individuals with congenital or postnatally acquired infection are needed to better understand the role of IFN-γ and its polymorphisms in the pathogenesis of ocular toxoplasmosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100099&lng=en&tlng=ensingle-nucleotide polymorphismsIFN- γ - Toxoplasma gondiiuveitisretinochoroiditis |
| spellingShingle | Ricardo Guerra Peixe Marcela Santana Bastos Boechat Alba Lucinia Peixoto Rangel Rhonia Franca Gomes Rosa Maria Luiza Petzl-Erler Lilian MG Bahia-Oliveira Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection Memorias do Instituto Oswaldo Cruz single-nucleotide polymorphisms IFN- γ - Toxoplasma gondii uveitis retinochoroiditis |
| title | Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection |
| title_full | Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection |
| title_fullStr | Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection |
| title_full_unstemmed | Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection |
| title_short | Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection |
| title_sort | single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by toxoplasma gondii infection |
| topic | single-nucleotide polymorphisms IFN- γ - Toxoplasma gondii uveitis retinochoroiditis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100099&lng=en&tlng=en |
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