HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity
<i>Sambucus formosana</i> is most commonly used as a traditional herb medicine in Taiwan. In this study, high performance liquid chromatography equipped with photodiode array detection-mass (HPLC−DAD-ESI-MS) method was developed for the identification and quantification of bioa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/21/3861 |
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| author | Ho-Shin Huang Hsu-Sheng Yu Chia-Hung Yen Ean-Tun Liaw |
| author_facet | Ho-Shin Huang Hsu-Sheng Yu Chia-Hung Yen Ean-Tun Liaw |
| author_sort | Ho-Shin Huang |
| collection | DOAJ |
| description | <i>Sambucus formosana</i> is most commonly used as a traditional herb medicine in Taiwan. In this study, high performance liquid chromatography equipped with photodiode array detection-mass (HPLC−DAD-ESI-MS) method was developed for the identification and quantification of bioactive phenolics. The developed method was also validated for accuracy, precision, limit of detection, and quantification. In this method, chlorogenic acid, rutin, isoquercetrin, nictoflorin, astragalin, and quercetin were quantified in linearity range of 10−100 (μg/mL) with a correlation coefficient of greater than 0.996. High recovery (86.5−93.1%) and good reproducibility were obtained for six phenolics with the relative standard deviation ranging from 1.7−3.1%. Therefore, the proposed method for simultaneous quantification of six bioactive phenolics in the extract and fractions of <i>S. formosana</i> using HPLC−DAD-ESI-MS detection under the optimized conditions is accurate and validated. Among the results, methanol extract showed the greatest values of total phenolic content (93.1 mg gallic acid equivalent/g). Additionally, the methanol extract revealed best antioxidant capacity based on the DPPH scavenging activity and anti-glycation activity (IC<sub>50</sub> was observed at 97.1 and 77.9 μg/mL, respectively). |
| first_indexed | 2024-12-10T09:21:53Z |
| format | Article |
| id | doaj.art-12f6d0000d7149468da2bf2b47601dec |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T09:21:53Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-12f6d0000d7149468da2bf2b47601dec2022-12-22T01:54:40ZengMDPI AGMolecules1420-30492019-10-012421386110.3390/molecules24213861molecules24213861HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation ActivityHo-Shin Huang0Hsu-Sheng Yu1Chia-Hung Yen2Ean-Tun Liaw3R&D Center, King Herb BioMed., Tainan 71201, TaiwanDepartment of Food Science, National Pingtung University of Science & Technology, Pingtung 91201, TaiwanDepartment of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, TaiwanDepartment of Food Science, National Pingtung University of Science & Technology, Pingtung 91201, Taiwan<i>Sambucus formosana</i> is most commonly used as a traditional herb medicine in Taiwan. In this study, high performance liquid chromatography equipped with photodiode array detection-mass (HPLC−DAD-ESI-MS) method was developed for the identification and quantification of bioactive phenolics. The developed method was also validated for accuracy, precision, limit of detection, and quantification. In this method, chlorogenic acid, rutin, isoquercetrin, nictoflorin, astragalin, and quercetin were quantified in linearity range of 10−100 (μg/mL) with a correlation coefficient of greater than 0.996. High recovery (86.5−93.1%) and good reproducibility were obtained for six phenolics with the relative standard deviation ranging from 1.7−3.1%. Therefore, the proposed method for simultaneous quantification of six bioactive phenolics in the extract and fractions of <i>S. formosana</i> using HPLC−DAD-ESI-MS detection under the optimized conditions is accurate and validated. Among the results, methanol extract showed the greatest values of total phenolic content (93.1 mg gallic acid equivalent/g). Additionally, the methanol extract revealed best antioxidant capacity based on the DPPH scavenging activity and anti-glycation activity (IC<sub>50</sub> was observed at 97.1 and 77.9 μg/mL, respectively).https://www.mdpi.com/1420-3049/24/21/3861<i>sambucus formosana</i>anti-glycationphenolics |
| spellingShingle | Ho-Shin Huang Hsu-Sheng Yu Chia-Hung Yen Ean-Tun Liaw HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity Molecules <i>sambucus formosana</i> anti-glycation phenolics |
| title | HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity |
| title_full | HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity |
| title_fullStr | HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity |
| title_full_unstemmed | HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity |
| title_short | HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity |
| title_sort | hplc dad esi ms analysis for simultaneous quantitation of phenolics in taiwan elderberry and its anti glycation activity |
| topic | <i>sambucus formosana</i> anti-glycation phenolics |
| url | https://www.mdpi.com/1420-3049/24/21/3861 |
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