Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction
Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain R...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2024-01-01
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| Series: | Media Kedokteran Hewan |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/MKH/article/view/47047 |
| _version_ | 1797348338665783296 |
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| author | Probo Probo Warih Tatag Pinayungan Aprinda Ratna Lovela Eduardus Bimo Aksono Ismudiono Boedi Setiawan Kadek Rachmawati Jola Rahmahani |
| author_facet | Probo Probo Warih Tatag Pinayungan Aprinda Ratna Lovela Eduardus Bimo Aksono Ismudiono Boedi Setiawan Kadek Rachmawati Jola Rahmahani |
| author_sort | Probo Probo Warih Tatag Pinayungan |
| collection | DOAJ |
| description | Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults. |
| first_indexed | 2024-03-08T12:04:26Z |
| format | Article |
| id | doaj.art-12f7b91905844c4a9d4477bcd1d24e51 |
| institution | Directory Open Access Journal |
| issn | 0215-8930 2775-975X |
| language | English |
| last_indexed | 2024-03-08T12:04:26Z |
| publishDate | 2024-01-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Media Kedokteran Hewan |
| spelling | doaj.art-12f7b91905844c4a9d4477bcd1d24e512024-01-23T10:17:15ZengUniversitas AirlanggaMedia Kedokteran Hewan0215-89302775-975X2024-01-01351212710.20473/mkh.v35i1.2024.21-2745133Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain ReactionProbo Probo Warih Tatag Pinayungan0Aprinda Ratna Lovela1https://orcid.org/0009-0009-1703-3478Eduardus Bimo Aksono2https://orcid.org/0000-0001-6961-2556Ismudiono3Boedi Setiawan4Kadek Rachmawati5Jola Rahmahani6Faculty of Veterinary Medicine, Universitas AirlanggaFaculty of Veterinary Medicine, Universitas AirlanggaFaculty of Veterinary Medicine, Universitas AirlanggaFaculty of Veterinary Medicine, Universitas AirlanggaFaculty of Veterinary Medicine, Universitas AirlanggaFaculty of Veterinary Medicine, Universitas AirlanggaFaculty of Veterinary Medicine, Universitas AirlanggaSex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults.https://e-journal.unair.ac.id/MKH/article/view/47047aratinga solstitialispcrsex determination |
| spellingShingle | Probo Probo Warih Tatag Pinayungan Aprinda Ratna Lovela Eduardus Bimo Aksono Ismudiono Boedi Setiawan Kadek Rachmawati Jola Rahmahani Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction Media Kedokteran Hewan aratinga solstitialis pcr sex determination |
| title | Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction |
| title_full | Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction |
| title_fullStr | Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction |
| title_full_unstemmed | Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction |
| title_short | Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction |
| title_sort | sex identification of the sun conure aratinga solstitialis using calamus based on polymerase chain reaction |
| topic | aratinga solstitialis pcr sex determination |
| url | https://e-journal.unair.ac.id/MKH/article/view/47047 |
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