Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window
The skin is heterogeneous and exerts strong scattering and aberration onto excitation light in multiphoton microscopy (MPM). Shifting to longer excitation wavelengths may help reduce skin scattering and aberration, potentially enabling larger imaging depths. However, previous demonstrations of skin...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
World Scientific Publishing
2023-07-01
|
Series: | Journal of Innovative Optical Health Sciences |
Subjects: | |
Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545822430040 |
_version_ | 1827898382102822912 |
---|---|
author | Xinlin Chen Yi Pan Ping Qiu Ke Wang |
author_facet | Xinlin Chen Yi Pan Ping Qiu Ke Wang |
author_sort | Xinlin Chen |
collection | DOAJ |
description | The skin is heterogeneous and exerts strong scattering and aberration onto excitation light in multiphoton microscopy (MPM). Shifting to longer excitation wavelengths may help reduce skin scattering and aberration, potentially enabling larger imaging depths. However, previous demonstrations of skin MPM employ excitation wavelengths only up to the 1700[Formula: see text]nm window, leaving an open question as to whether longer excitation wavelengths are suitable for deep-skin MPM. Here, in order to explore the longer-wavelength territory, first, we demonstrate characterization of the broadband transmittance of excised mouse skin, revealing a high transmittance window at 2200[Formula: see text]nm. Then, we demonstrate third-harmonic generation (THG) imaging in mouse skin in vivo excited at this window. With 9[Formula: see text]mW optical power on the skin surface operating at 1[Formula: see text]MHz repetition rate, we can get THG signals of 250[Formula: see text][Formula: see text]m below the skin surface. Comparative THG imaging excited at the 1700[Formula: see text]nm window shows that as imaging depth increases, THG signals decay even faster than those excited at 2200[Formula: see text]nm. Our results thus uncover the 2200[Formula: see text]nm window as a new, promising excitation window potential for deep-skin MPM. |
first_indexed | 2024-03-12T23:02:24Z |
format | Article |
id | doaj.art-12f8a41c220b48c69b31b90a7d9c6fa7 |
institution | Directory Open Access Journal |
issn | 1793-5458 1793-7205 |
language | English |
last_indexed | 2024-03-12T23:02:24Z |
publishDate | 2023-07-01 |
publisher | World Scientific Publishing |
record_format | Article |
series | Journal of Innovative Optical Health Sciences |
spelling | doaj.art-12f8a41c220b48c69b31b90a7d9c6fa72023-07-19T08:37:30ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-07-01160410.1142/S1793545822430040Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm windowXinlin Chen0Yi Pan1Ping Qiu2Ke Wang3Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. ChinaThe skin is heterogeneous and exerts strong scattering and aberration onto excitation light in multiphoton microscopy (MPM). Shifting to longer excitation wavelengths may help reduce skin scattering and aberration, potentially enabling larger imaging depths. However, previous demonstrations of skin MPM employ excitation wavelengths only up to the 1700[Formula: see text]nm window, leaving an open question as to whether longer excitation wavelengths are suitable for deep-skin MPM. Here, in order to explore the longer-wavelength territory, first, we demonstrate characterization of the broadband transmittance of excised mouse skin, revealing a high transmittance window at 2200[Formula: see text]nm. Then, we demonstrate third-harmonic generation (THG) imaging in mouse skin in vivo excited at this window. With 9[Formula: see text]mW optical power on the skin surface operating at 1[Formula: see text]MHz repetition rate, we can get THG signals of 250[Formula: see text][Formula: see text]m below the skin surface. Comparative THG imaging excited at the 1700[Formula: see text]nm window shows that as imaging depth increases, THG signals decay even faster than those excited at 2200[Formula: see text]nm. Our results thus uncover the 2200[Formula: see text]nm window as a new, promising excitation window potential for deep-skin MPM.https://www.worldscientific.com/doi/10.1142/S1793545822430040Third-harmonic generation2200nm1700nmskin |
spellingShingle | Xinlin Chen Yi Pan Ping Qiu Ke Wang Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window Journal of Innovative Optical Health Sciences Third-harmonic generation 2200nm 1700nm skin |
title | Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window |
title_full | Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window |
title_fullStr | Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window |
title_full_unstemmed | Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window |
title_short | Deep-skin third-harmonic generation (THG) imaging in vivo excited at the 2200 nm window |
title_sort | deep skin third harmonic generation thg imaging in vivo excited at the 2200 nm window |
topic | Third-harmonic generation 2200nm 1700nm skin |
url | https://www.worldscientific.com/doi/10.1142/S1793545822430040 |
work_keys_str_mv | AT xinlinchen deepskinthirdharmonicgenerationthgimaginginvivoexcitedatthe2200nmwindow AT yipan deepskinthirdharmonicgenerationthgimaginginvivoexcitedatthe2200nmwindow AT pingqiu deepskinthirdharmonicgenerationthgimaginginvivoexcitedatthe2200nmwindow AT kewang deepskinthirdharmonicgenerationthgimaginginvivoexcitedatthe2200nmwindow |