Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats
A challenge in the study of gastrointestinal microbiota (GITm) is the validation of the genomic data with metabolic studies of the microbial communities to understand how the microbial networks work during health and sickness. To gain insights into the metabolism of the GITm, feces from healthy and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.949272/full |
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| author | Betsy Anaid Peña-Ocaña Yuki Hoshiko Mayel Silva-Flores Toshinari Maeda Israel Pérez-Torres Rodolfo García-Contreras Wilbert Gutiérrez-Sarmiento Luz Hernández-Esquivel Álvaro Marín-Hernández Rosina Sánchez-Thomas Emma Saavedra José Salud Rodríguez-Zavala Ricardo Jasso-Chávez |
| author_facet | Betsy Anaid Peña-Ocaña Yuki Hoshiko Mayel Silva-Flores Toshinari Maeda Israel Pérez-Torres Rodolfo García-Contreras Wilbert Gutiérrez-Sarmiento Luz Hernández-Esquivel Álvaro Marín-Hernández Rosina Sánchez-Thomas Emma Saavedra José Salud Rodríguez-Zavala Ricardo Jasso-Chávez |
| author_sort | Betsy Anaid Peña-Ocaña |
| collection | DOAJ |
| description | A challenge in the study of gastrointestinal microbiota (GITm) is the validation of the genomic data with metabolic studies of the microbial communities to understand how the microbial networks work during health and sickness. To gain insights into the metabolism of the GITm, feces from healthy and sick rats with cancer were inoculated in a defined synthetic medium directed for anaerobic prokaryote growth (INC-07 medium). Significant differences between cultures of healthy and sick individuals were found: 1) the consumption of the carbon source and the enzyme activity involved in their catabolism (e.g., sucrase, lactase, lipases, aminotransferases, and dehydrogenases); 2) higher excretion of acetic, propionic, isobutyric, butyric, valeric, and isovaleric acids; 3) methane production; 4) ability to form biofilms; and 5) up to 500 amplicon sequencing variants (ASVs) identified showed different diversity and abundance. Moreover, the bowel inflammation induced by cancer triggered oxidative stress, which correlated with deficient antioxidant machinery (e.g., NADPH-producing enzymes) determined in the GITm cultures from sick individuals in comparison with those from control individuals. Altogether, the data suggested that to preserve the microbial network between bacteria and methanogenic archaea, a complete oxidation of the carbon source may be essential for healthy microbiota. The correlation of 16S rRNA gene metabarcoding between cultures and feces, as well as metabolomic data found in cultures, suggest that INC-07 medium may be a useful tool to understand the metabolism of microbiota under gut conditions. |
| first_indexed | 2024-04-11T12:47:06Z |
| format | Article |
| id | doaj.art-12faf5372af44baf97086cd93b616caf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T12:47:06Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-12faf5372af44baf97086cd93b616caf2022-12-22T04:23:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.949272949272Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing ratsBetsy Anaid Peña-Ocaña0Yuki Hoshiko1Mayel Silva-Flores2Toshinari Maeda3Israel Pérez-Torres4Rodolfo García-Contreras5Wilbert Gutiérrez-Sarmiento6Luz Hernández-Esquivel7Álvaro Marín-Hernández8Rosina Sánchez-Thomas9Emma Saavedra10José Salud Rodríguez-Zavala11Ricardo Jasso-Chávez12Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDivision of Environment-Conscious Chemistry and Bioengineering, Department of Biological Functions Engineering, Kyushu Institute of Technology, Kitakyushu, JapanDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDivision of Environment-Conscious Chemistry and Bioengineering, Department of Biological Functions Engineering, Kyushu Institute of Technology, Kitakyushu, JapanDepartamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoInstituto Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Chiapas, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoA challenge in the study of gastrointestinal microbiota (GITm) is the validation of the genomic data with metabolic studies of the microbial communities to understand how the microbial networks work during health and sickness. To gain insights into the metabolism of the GITm, feces from healthy and sick rats with cancer were inoculated in a defined synthetic medium directed for anaerobic prokaryote growth (INC-07 medium). Significant differences between cultures of healthy and sick individuals were found: 1) the consumption of the carbon source and the enzyme activity involved in their catabolism (e.g., sucrase, lactase, lipases, aminotransferases, and dehydrogenases); 2) higher excretion of acetic, propionic, isobutyric, butyric, valeric, and isovaleric acids; 3) methane production; 4) ability to form biofilms; and 5) up to 500 amplicon sequencing variants (ASVs) identified showed different diversity and abundance. Moreover, the bowel inflammation induced by cancer triggered oxidative stress, which correlated with deficient antioxidant machinery (e.g., NADPH-producing enzymes) determined in the GITm cultures from sick individuals in comparison with those from control individuals. Altogether, the data suggested that to preserve the microbial network between bacteria and methanogenic archaea, a complete oxidation of the carbon source may be essential for healthy microbiota. The correlation of 16S rRNA gene metabarcoding between cultures and feces, as well as metabolomic data found in cultures, suggest that INC-07 medium may be a useful tool to understand the metabolism of microbiota under gut conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2022.949272/fullanaerobic cultureionomicsmethane productionoxidative stressbiofilmmetabarcoding |
| spellingShingle | Betsy Anaid Peña-Ocaña Yuki Hoshiko Mayel Silva-Flores Toshinari Maeda Israel Pérez-Torres Rodolfo García-Contreras Wilbert Gutiérrez-Sarmiento Luz Hernández-Esquivel Álvaro Marín-Hernández Rosina Sánchez-Thomas Emma Saavedra José Salud Rodríguez-Zavala Ricardo Jasso-Chávez Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats Frontiers in Microbiology anaerobic culture ionomics methane production oxidative stress biofilm metabarcoding |
| title | Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats |
| title_full | Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats |
| title_fullStr | Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats |
| title_full_unstemmed | Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats |
| title_short | Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats |
| title_sort | cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma bearing rats |
| topic | anaerobic culture ionomics methane production oxidative stress biofilm metabarcoding |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.949272/full |
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