Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model
Abstract Background Type 2 diabetes mellitus is characterized by insulin resistance and pancreatic β-cell dysfunction. A decrease in β-cell mass, which occurs during the progression of Type 2 diabetes mellitus, contributes to impaired insulin secretion. Mulberry leaves contain various nutritional co...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-020-02933-4 |
| _version_ | 1819140641307754496 |
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| author | Patlada Suthamwong Manabu Minami Toshiaki Okada Nonomi Shiwaku Mai Uesugi Masayuki Yokode Kaeko Kamei |
| author_facet | Patlada Suthamwong Manabu Minami Toshiaki Okada Nonomi Shiwaku Mai Uesugi Masayuki Yokode Kaeko Kamei |
| author_sort | Patlada Suthamwong |
| collection | DOAJ |
| description | Abstract Background Type 2 diabetes mellitus is characterized by insulin resistance and pancreatic β-cell dysfunction. A decrease in β-cell mass, which occurs during the progression of Type 2 diabetes mellitus, contributes to impaired insulin secretion. Mulberry leaves contain various nutritional components that exert anti-diabetic and anti-atherogenic effects. The present study analyzed the effects of mulberry leaf intake on pancreatic β-cells to clarify the mechanisms underlying its anti-diabetic function. Methods Mulberry leaves (Morus alba L.) were dried at 180 °C for 8 s in a hot-air mill and fed to obesity/Type 2 diabetes mellitus db/db mouse models at 5% (w/w) as part of a normal diet from 7 to 10, 15, or 20 weeks of age. An intraperitoneal glucose tolerance test was then performed on the mice. To evaluate the β-cell mass, the pancreas was subjected to immunohistological analysis with an anti-insulin antibody. A TUNEL assay and immunohistological analysis with a proliferation marker was also performed. Expression levels of endoplasmic reticulum stress-responsible genes and proliferation markers were assessed by quantitative RT-PCR. Results Intake of mulberry leaves maintained the β-cell function of db/db mice. Moreover, oral administration of mulberry leaves significantly decreased cell death by reducing endoplasmic reticulum stress in the pancreas. Mulberry leaves significantly increased proliferation of β-cells and the expression of pancreatic duodenal homeobox1 mRNA in the pancreas. Conclusion Considered together, these results indicate that dietary mulberry leaf administration can maintain insulin levels and pancreatic β-cell mass, at least in part, by suppressing endoplasmic reticulum stress in Type 2 diabetes mellitus mouse models. |
| first_indexed | 2024-12-22T11:41:47Z |
| format | Article |
| id | doaj.art-12fb102d741b41e9ac1e43c903051cba |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-22T11:41:47Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-12fb102d741b41e9ac1e43c903051cba2022-12-21T18:27:15ZengBMCBMC Complementary Medicine and Therapies2662-76712020-05-0120111010.1186/s12906-020-02933-4Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse modelPatlada Suthamwong0Manabu Minami1Toshiaki Okada2Nonomi Shiwaku3Mai Uesugi4Masayuki Yokode5Kaeko Kamei6Department of Functional Chemistry, Kyoto Institute of TechnologyDepartment of Clinical Innovative Medicine, Kyoto University Graduate School of MedicineDepartment of Functional Chemistry, Kyoto Institute of TechnologyDepartment of Functional Chemistry, Kyoto Institute of TechnologyDepartment of Functional Chemistry, Kyoto Institute of TechnologyDepartment of Clinical Innovative Medicine, Kyoto University Graduate School of MedicineDepartment of Functional Chemistry, Kyoto Institute of TechnologyAbstract Background Type 2 diabetes mellitus is characterized by insulin resistance and pancreatic β-cell dysfunction. A decrease in β-cell mass, which occurs during the progression of Type 2 diabetes mellitus, contributes to impaired insulin secretion. Mulberry leaves contain various nutritional components that exert anti-diabetic and anti-atherogenic effects. The present study analyzed the effects of mulberry leaf intake on pancreatic β-cells to clarify the mechanisms underlying its anti-diabetic function. Methods Mulberry leaves (Morus alba L.) were dried at 180 °C for 8 s in a hot-air mill and fed to obesity/Type 2 diabetes mellitus db/db mouse models at 5% (w/w) as part of a normal diet from 7 to 10, 15, or 20 weeks of age. An intraperitoneal glucose tolerance test was then performed on the mice. To evaluate the β-cell mass, the pancreas was subjected to immunohistological analysis with an anti-insulin antibody. A TUNEL assay and immunohistological analysis with a proliferation marker was also performed. Expression levels of endoplasmic reticulum stress-responsible genes and proliferation markers were assessed by quantitative RT-PCR. Results Intake of mulberry leaves maintained the β-cell function of db/db mice. Moreover, oral administration of mulberry leaves significantly decreased cell death by reducing endoplasmic reticulum stress in the pancreas. Mulberry leaves significantly increased proliferation of β-cells and the expression of pancreatic duodenal homeobox1 mRNA in the pancreas. Conclusion Considered together, these results indicate that dietary mulberry leaf administration can maintain insulin levels and pancreatic β-cell mass, at least in part, by suppressing endoplasmic reticulum stress in Type 2 diabetes mellitus mouse models.http://link.springer.com/article/10.1186/s12906-020-02933-4Mulberry leavesβ-CellEndoplasmic reticulum stressObesityType 2 diabetes |
| spellingShingle | Patlada Suthamwong Manabu Minami Toshiaki Okada Nonomi Shiwaku Mai Uesugi Masayuki Yokode Kaeko Kamei Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model BMC Complementary Medicine and Therapies Mulberry leaves β-Cell Endoplasmic reticulum stress Obesity Type 2 diabetes |
| title | Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model |
| title_full | Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model |
| title_fullStr | Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model |
| title_full_unstemmed | Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model |
| title_short | Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model |
| title_sort | administration of mulberry leaves maintains pancreatic β cell mass in obese type 2 diabetes mellitus mouse model |
| topic | Mulberry leaves β-Cell Endoplasmic reticulum stress Obesity Type 2 diabetes |
| url | http://link.springer.com/article/10.1186/s12906-020-02933-4 |
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