Prevalence of exoT Gene in Pseudomonas aeruginosa Isolated from Various Clinical Samples: A Cross-sectional Study
Introduction: Pseudomonas aeruginosa (P. aeruginosa) is one of the most frequently co-infecting bacteria reported. Development of drug resistance, biofilm formation, cell associated factors make the P. aeruginosa more virulent. Type III secretion system controls expression of genes. P. aeruginos...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2021-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/15742/52171_CE[Ra1]_F[SH]_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdf |
| Summary: | Introduction: Pseudomonas aeruginosa (P. aeruginosa) is one of
the most frequently co-infecting bacteria reported. Development
of drug resistance, biofilm formation, cell associated factors
make the P. aeruginosa more virulent. Type III secretion system
controls expression of genes. P. aeruginosa chromosome
harbours exoT, exoS, exoU, and exoY virulent genes. Gene
exoT plays an important role in causation of infection. The
identification of virulent markers of pathogens for identification
of acute and chronic infections at early stage remains a critical
area and still need large research.
Aim: To study the prevalence of exoT gene encoding exotoxin T
in P. aeruginosa from clinical samples.
Materials and Methods: A descriptive cross-sectional research
was conducted during January 2015 to March 2016 at the
Department of Microbiology in Dr. D. Y. Patil Medical College,
Pimpri, Pune, Maharashtra, India. Various clinical samples
were processed using standard laboratory methods. The
statistical analysis was done by using Chi-square test. Strains
of P. aeruginosa isolated from various clinical samples were
identified using standard laboratory methods, and exoT gene
was detected by Polymerase Chain Reaction (PCR) and gel
electrophoresis technique.
Results: Out of 30 strains of P. aeruginosa, 20 (66.67%) were
isolated from male and 10 (33.33%) from female patients. Most
of them belonged to the age group 41-60 years (46.67%). The
exoT gene occurred in 20 (66.67%) isolates of P. aeruginosa,
while 10 (33.33%) showed negative amplification results. Out
of 20 exoT genes in P. aeruginosa, 17/20 (85%) were detected
from male and 3/10 (15%) from female patients.
Conclusion: Gene exoT of P. aeruginosa plays the crucial role
in causation of disease. It is concluded that exoT gene can be a
notable virulent element expressed by 66.67% of P. aeruginosa
clinical isolates. The proven role of exoT virulence gene in the
pathogenicity of P. aeruginosa would help in understanding the
prognosis of Pseudomonas infection and designing an effective
treatment and vaccine against the Pseudomonas infections to
prevent them. |
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| ISSN: | 2249-782X 0973-709X |