Evaluation of antioxidant activity of Melittis melissophyllum L. extracts
The antioxidant activities of methanol and ethanol (10%, 30%, 50% and 96%) extracts of the aerial parts of Melittis melissophyllum L. were determined by DPP H, ABTS and FRAP assays. The content of flavonoids and phenols was also investigated. The total phenolic content in the extracts was d...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2014-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641404401G.pdf |
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| author | Grujić Slavica M. Stojanović Gordana S. Mitić Violeta D. Stankov-Jovanović Vesna Džamić Ana M. Alimpić Ana Z. Marin Petar D. |
| author_facet | Grujić Slavica M. Stojanović Gordana S. Mitić Violeta D. Stankov-Jovanović Vesna Džamić Ana M. Alimpić Ana Z. Marin Petar D. |
| author_sort | Grujić Slavica M. |
| collection | DOAJ |
| description | The antioxidant activities of methanol and ethanol (10%, 30%, 50% and 96%)
extracts of the aerial parts of Melittis melissophyllum L. were determined by
DPP H, ABTS and FRAP assays. The content of flavonoids and phenols was also
investigated. The total phenolic content in the extracts was determined using
Folin-Ciocalteu assay; their amounts ranged between 63.5 and 111.7 mg
GAeqv/g, while the concentrations of flavonoids were from 7.33 to 56.00 mg
Queqv/g. IC50 values of the DPP H scavenging effect were from 0.109 to 0.664
mg/mL. The DPP H scavenging effect of the extracts was determined and the
obtained IC50 values were from 0.109 to 0.664 mg/mL of solution. The values
of ABTS radical activity were from 0.45 to 0.89 mg ascorbic acid/g. The FRAP
value was within the range 0.160-0.382 mmol Fe/mg. The obtained values were
analyzed by means of multivariate analysis, employing a hierarchical cluster
analysis and between-groups linkage. The presented results confirmed that M.
melissophyllum possesses good antioxidant properties and may serve as a
promising source of natural antioxidants. [Projekat Ministarstva nauke
Republike Srbije, br. 173029] |
| first_indexed | 2024-12-10T08:27:39Z |
| format | Article |
| id | doaj.art-12fdec29b0e14c7c93cd9c6be00cbfd6 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-10T08:27:39Z |
| publishDate | 2014-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-12fdec29b0e14c7c93cd9c6be00cbfd62022-12-22T01:56:11ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-016641401141010.2298/ABS1404401G0354-46641404401GEvaluation of antioxidant activity of Melittis melissophyllum L. extractsGrujić Slavica M.0Stojanović Gordana S.1Mitić Violeta D.2Stankov-Jovanović Vesna3Džamić Ana M.4Alimpić Ana Z.5Marin Petar D.6Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeFaculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeFaculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, BelgradeThe antioxidant activities of methanol and ethanol (10%, 30%, 50% and 96%) extracts of the aerial parts of Melittis melissophyllum L. were determined by DPP H, ABTS and FRAP assays. The content of flavonoids and phenols was also investigated. The total phenolic content in the extracts was determined using Folin-Ciocalteu assay; their amounts ranged between 63.5 and 111.7 mg GAeqv/g, while the concentrations of flavonoids were from 7.33 to 56.00 mg Queqv/g. IC50 values of the DPP H scavenging effect were from 0.109 to 0.664 mg/mL. The DPP H scavenging effect of the extracts was determined and the obtained IC50 values were from 0.109 to 0.664 mg/mL of solution. The values of ABTS radical activity were from 0.45 to 0.89 mg ascorbic acid/g. The FRAP value was within the range 0.160-0.382 mmol Fe/mg. The obtained values were analyzed by means of multivariate analysis, employing a hierarchical cluster analysis and between-groups linkage. The presented results confirmed that M. melissophyllum possesses good antioxidant properties and may serve as a promising source of natural antioxidants. [Projekat Ministarstva nauke Republike Srbije, br. 173029]http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641404401G.pdfMelittis melissophyllumLamiaceaeextractsphenolsflavonoidsDPP HABTSFRAP |
| spellingShingle | Grujić Slavica M. Stojanović Gordana S. Mitić Violeta D. Stankov-Jovanović Vesna Džamić Ana M. Alimpić Ana Z. Marin Petar D. Evaluation of antioxidant activity of Melittis melissophyllum L. extracts Archives of Biological Sciences Melittis melissophyllum Lamiaceae extracts phenols flavonoids DPP H ABTS FRAP |
| title | Evaluation of antioxidant activity of Melittis melissophyllum L. extracts |
| title_full | Evaluation of antioxidant activity of Melittis melissophyllum L. extracts |
| title_fullStr | Evaluation of antioxidant activity of Melittis melissophyllum L. extracts |
| title_full_unstemmed | Evaluation of antioxidant activity of Melittis melissophyllum L. extracts |
| title_short | Evaluation of antioxidant activity of Melittis melissophyllum L. extracts |
| title_sort | evaluation of antioxidant activity of melittis melissophyllum l extracts |
| topic | Melittis melissophyllum Lamiaceae extracts phenols flavonoids DPP H ABTS FRAP |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641404401G.pdf |
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