Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts
Sweet cherry (Prunus avium L.) stems in the form of infusions and decoctions are traditionally consumed for diuretic and anti-inflammatory purposes. This study aimed to evaluate antimicrobial and antibiofilm activity of ethanolic and methanolic extract made from sweet cherry stems. Extracts are obt...
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| Format: | Article |
| Language: | English |
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University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2022-06-01
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| Series: | Genetics & Applications |
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| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/82 |
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| author | Irma Mahmutović-Dizdarević Mirsada Salihović Biljana Radusin Sopić Anesa Jerković-Mujkić Mirsada Hukić Monia Avdić |
| author_facet | Irma Mahmutović-Dizdarević Mirsada Salihović Biljana Radusin Sopić Anesa Jerković-Mujkić Mirsada Hukić Monia Avdić |
| author_sort | Irma Mahmutović-Dizdarević |
| collection | DOAJ |
| description |
Sweet cherry (Prunus avium L.) stems in the form of infusions and decoctions are traditionally consumed for diuretic and anti-inflammatory purposes. This study aimed to evaluate antimicrobial and antibiofilm activity of ethanolic and methanolic extract made from sweet cherry stems. Extracts are obtained by the Soxhlet extraction and maceration procedures. For the determination of the minimum inhibitory concentration, the broth microdilution method is employed, and the assessment of the microbiocidal activity of the extracts is conducted. The antibiofilm activity was tested through the tissue culture plate method, which also allowed the determination of the biofilm-forming categories of investigated strains. The final step involved the calculation of the biofilm inhibition percentage. Examined extracts with the balanced activity inhibited the growth of all microorganisms, with Gram-negative bacteria being more sensitive in comparison to Gram-positive. The values of the minimum inhibitory concentration were 125 µg/ml, and 250 µg/ml, respectfully. Candida albicans was the most susceptible and the minimum inhibitory concentration of both extracts was 62.50 µg/ml. The microbiocidal activity of the extracts was not recorded. Extracts exhibited different impacts on the biofilm-forming capacity of the investigated microbes, and both inhibition and stimulation effects are noted. The percentage of the biofilm inhibition was from 14.27% to 84.78%, with the highest inhibition recorded for the multidrug-resistant Escherichia coli, treated with the ethanolic extract. Sweet cherry stems are a valuable source of natural bioactive compounds, but their usage in the treatment of microbial infections should be correctly and carefully implemented.
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| first_indexed | 2024-04-11T06:31:18Z |
| format | Article |
| id | doaj.art-130189fdb34644329ea4436faee35050 |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-11T06:31:18Z |
| publishDate | 2022-06-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-130189fdb34644329ea4436faee350502022-12-22T04:40:01ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2022-06-016110.31383/ga.vol6iss1pp38-51Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extractsIrma Mahmutović-Dizdarević0Mirsada Salihović1Biljana Radusin Sopić2Anesa Jerković-Mujkić3Mirsada Hukić4Monia Avdić5University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Natural Sciences and Mathematics, Department of Biology, Dr. Mladena Stojanovića 2, 78000 Banja Luka, Bosnia and Herzegovina University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and HerzegovinaAcademy of Sciences and Arts of Bosnia and Herzegovina, Center for Disease Control and Geohealth Studies, Bistrik 7, 71000 Sarajevo, Bosnia and Herzegovina, Institute for Biomedical Diagnostics and Research Nalaz, Čekaluša 69, 71000 Sarajevo, Bosnia and HerzegovinaAcademy of Sciences and Arts of Bosnia and Herzegovina, Center for Disease Control and Geohealth Studies, Bistrik 7, 71000 Sarajevo, Bosnia and Herzegovina, International Burch University (IBU), Department of Genetics and Bioengineering, Francuske revolucije bb, 71210 Ilidža, Bosnia and Herzegovina Sweet cherry (Prunus avium L.) stems in the form of infusions and decoctions are traditionally consumed for diuretic and anti-inflammatory purposes. This study aimed to evaluate antimicrobial and antibiofilm activity of ethanolic and methanolic extract made from sweet cherry stems. Extracts are obtained by the Soxhlet extraction and maceration procedures. For the determination of the minimum inhibitory concentration, the broth microdilution method is employed, and the assessment of the microbiocidal activity of the extracts is conducted. The antibiofilm activity was tested through the tissue culture plate method, which also allowed the determination of the biofilm-forming categories of investigated strains. The final step involved the calculation of the biofilm inhibition percentage. Examined extracts with the balanced activity inhibited the growth of all microorganisms, with Gram-negative bacteria being more sensitive in comparison to Gram-positive. The values of the minimum inhibitory concentration were 125 µg/ml, and 250 µg/ml, respectfully. Candida albicans was the most susceptible and the minimum inhibitory concentration of both extracts was 62.50 µg/ml. The microbiocidal activity of the extracts was not recorded. Extracts exhibited different impacts on the biofilm-forming capacity of the investigated microbes, and both inhibition and stimulation effects are noted. The percentage of the biofilm inhibition was from 14.27% to 84.78%, with the highest inhibition recorded for the multidrug-resistant Escherichia coli, treated with the ethanolic extract. Sweet cherry stems are a valuable source of natural bioactive compounds, but their usage in the treatment of microbial infections should be correctly and carefully implemented. https://genapp.ba/editions/index.php/journal/article/view/82antibiofilm activityplant antimicrobialsPrunus aviumstems extracts |
| spellingShingle | Irma Mahmutović-Dizdarević Mirsada Salihović Biljana Radusin Sopić Anesa Jerković-Mujkić Mirsada Hukić Monia Avdić Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts Genetics & Applications antibiofilm activity plant antimicrobials Prunus avium stems extracts |
| title | Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts |
| title_full | Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts |
| title_fullStr | Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts |
| title_full_unstemmed | Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts |
| title_short | Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts |
| title_sort | evaluation of the antimicrobial and antibiofilm activity of sweet cherry prunus avium l stems extracts |
| topic | antibiofilm activity plant antimicrobials Prunus avium stems extracts |
| url | https://genapp.ba/editions/index.php/journal/article/view/82 |
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