Quantification of virus-infected cells using RNA FISH-Flow

Quantification of virus-infected cells using RNA FISH-Flow

Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA. RNA FISH-Flow probes can be synthesized to match a...

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Bibliographic Details
Main Authors: Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Microbiology
Molecular Biology
In Situ Hybridization
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002587

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http://www.sciencedirect.com/science/article/pii/S2666166723002587

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