Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation.
The growing body of clinical and experimental data regarding electromagnetic field (EMF) bioeffects and their therapeutic applications has contributed to a better understanding of the underlying mechanisms of action. This study reports that two EMF modalities currently in clinical use, a pulse-modul...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3625142?pdf=render |
| _version_ | 1819236397999980544 |
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| author | David Muehsam Parviz Lalezari Rukmani Lekhraj Provvidenza M Abruzzo Alessandra Bolotta Marina Marini Ferdinando Bersani Giorgio Aicardi Arthur Pilla Diana Casper |
| author_facet | David Muehsam Parviz Lalezari Rukmani Lekhraj Provvidenza M Abruzzo Alessandra Bolotta Marina Marini Ferdinando Bersani Giorgio Aicardi Arthur Pilla Diana Casper |
| author_sort | David Muehsam |
| collection | DOAJ |
| description | The growing body of clinical and experimental data regarding electromagnetic field (EMF) bioeffects and their therapeutic applications has contributed to a better understanding of the underlying mechanisms of action. This study reports that two EMF modalities currently in clinical use, a pulse-modulated radiofrequency (PRF) signal, and a static magnetic field (SMF), applied independently, increased the rate of deoxygenation of human hemoglobin (Hb) in a cell-free assay. Deoxygenation of Hb was initiated using the reducing agent dithiothreitol (DTT) in an assay that allowed the time for deoxygenation to be controlled (from several min to several hours) by adjusting the relative concentrations of DTT and Hb. The time course of Hb deoxygenation was observed using visible light spectroscopy. Exposure for 10-30 min to either PRF or SMF increased the rate of deoxygenation occurring several min to several hours after the end of EMF exposure. The sensitivity and biochemical simplicity of the assay developed here suggest a new research tool that may help to further the understanding of basic biophysical EMF transduction mechanisms. If the results of this study were to be shown to occur at the cellular and tissue level, EMF-enhanced oxygen availability would be one of the mechanisms by which clinically relevant EMF-mediated enhancement of growth and repair processes could occur. |
| first_indexed | 2024-12-23T13:03:48Z |
| format | Article |
| id | doaj.art-1302be570fb04003bb7d44de238b4783 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T13:03:48Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1302be570fb04003bb7d44de238b47832022-12-21T17:45:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6175210.1371/journal.pone.0061752Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation.David MuehsamParviz LalezariRukmani LekhrajProvvidenza M AbruzzoAlessandra BolottaMarina MariniFerdinando BersaniGiorgio AicardiArthur PillaDiana CasperThe growing body of clinical and experimental data regarding electromagnetic field (EMF) bioeffects and their therapeutic applications has contributed to a better understanding of the underlying mechanisms of action. This study reports that two EMF modalities currently in clinical use, a pulse-modulated radiofrequency (PRF) signal, and a static magnetic field (SMF), applied independently, increased the rate of deoxygenation of human hemoglobin (Hb) in a cell-free assay. Deoxygenation of Hb was initiated using the reducing agent dithiothreitol (DTT) in an assay that allowed the time for deoxygenation to be controlled (from several min to several hours) by adjusting the relative concentrations of DTT and Hb. The time course of Hb deoxygenation was observed using visible light spectroscopy. Exposure for 10-30 min to either PRF or SMF increased the rate of deoxygenation occurring several min to several hours after the end of EMF exposure. The sensitivity and biochemical simplicity of the assay developed here suggest a new research tool that may help to further the understanding of basic biophysical EMF transduction mechanisms. If the results of this study were to be shown to occur at the cellular and tissue level, EMF-enhanced oxygen availability would be one of the mechanisms by which clinically relevant EMF-mediated enhancement of growth and repair processes could occur.http://europepmc.org/articles/PMC3625142?pdf=render |
| spellingShingle | David Muehsam Parviz Lalezari Rukmani Lekhraj Provvidenza M Abruzzo Alessandra Bolotta Marina Marini Ferdinando Bersani Giorgio Aicardi Arthur Pilla Diana Casper Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation. PLoS ONE |
| title | Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation. |
| title_full | Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation. |
| title_fullStr | Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation. |
| title_full_unstemmed | Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation. |
| title_short | Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation. |
| title_sort | non thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell free preparation |
| url | http://europepmc.org/articles/PMC3625142?pdf=render |
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