Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain
Biotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms – destructors of phenol and their identification is relevant. The aim of this study was...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-02-01
|
| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2020/02/Gudzenko_1_20.pdf |
| _version_ | 1797427840749142016 |
|---|---|
| author | Т. V. Gudzenko O. V. Voliuvach O. G. Gorshkova А. М. Ostapchuk V. O. Ivanytsia |
| author_facet | Т. V. Gudzenko O. V. Voliuvach O. G. Gorshkova А. М. Ostapchuk V. O. Ivanytsia |
| author_sort | Т. V. Gudzenko |
| collection | DOAJ |
| description | Biotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms – destructors of phenol and their identification is relevant. The aim of this study was to determine phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 – destructor of phenol, isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried out using automatic system of microorganisms identification MIDI Sherlock based on gas chromatograph Agilent 7890. The phenol-oxidizing activity of bacteria was determined by photometric method according to the degree of phenol extraction from the water. It was experimentally confirmed that Brevibacillus sp. F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15 days of exposure. Chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%), a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain’s fatty acids profile. The total content of the branched saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, ∑(a-17:1/i-17:1) was 85.0% and that of saturated and unsaturated fatty acids with the normal structure – 7% of the total fatty acids pool. The distinction of Brevibacillus centrosporus F14 strain’s fatty acid profile from other bacteria of Brevibacillus genus was the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the minor content of fatty acids markers 16:0 N alcohol (0.60%), 18:3 w6c (0.4%) and ∑(a-17:1/i-17:1) (1.94%). According to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain belongs to Brevibacillus centrosporus species. The index of Brevibacillus centrosporus F14 strain’s fatty acid profile similarity with the library was 0.645. |
| first_indexed | 2024-03-09T08:49:38Z |
| format | Article |
| id | doaj.art-13032c334be7494a84e32bd08b85d2a2 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:49:38Z |
| publishDate | 2020-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-13032c334be7494a84e32bd08b85d2a22023-12-02T14:35:54ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032020-02-01921849110.15407/ubj92.01.084Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strainТ. V. Gudzenko0O. V. Voliuvach1O. G. Gorshkova2А. М. Ostapchuk3V. O. Ivanytsia4I.I. Mechnykov Оdessa National University, UkraineI.I. Mechnykov Оdessa National University, UkraineI.I. Mechnykov Оdessa National University, UkraineI.I. Mechnykov Оdessa National University, UkraineI.I. Mechnykov Оdessa National University, UkraineBiotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms – destructors of phenol and their identification is relevant. The aim of this study was to determine phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 – destructor of phenol, isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried out using automatic system of microorganisms identification MIDI Sherlock based on gas chromatograph Agilent 7890. The phenol-oxidizing activity of bacteria was determined by photometric method according to the degree of phenol extraction from the water. It was experimentally confirmed that Brevibacillus sp. F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15 days of exposure. Chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%), a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain’s fatty acids profile. The total content of the branched saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, ∑(a-17:1/i-17:1) was 85.0% and that of saturated and unsaturated fatty acids with the normal structure – 7% of the total fatty acids pool. The distinction of Brevibacillus centrosporus F14 strain’s fatty acid profile from other bacteria of Brevibacillus genus was the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the minor content of fatty acids markers 16:0 N alcohol (0.60%), 18:3 w6c (0.4%) and ∑(a-17:1/i-17:1) (1.94%). According to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain belongs to Brevibacillus centrosporus species. The index of Brevibacillus centrosporus F14 strain’s fatty acid profile similarity with the library was 0.645.http://ukrbiochemjournal.org/wp-content/uploads/2020/02/Gudzenko_1_20.pdfbrevibacillus centrosporuscomposition of fatty acidsdestructor of phenolidentification |
| spellingShingle | Т. V. Gudzenko O. V. Voliuvach O. G. Gorshkova А. М. Ostapchuk V. O. Ivanytsia Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain The Ukrainian Biochemical Journal brevibacillus centrosporus composition of fatty acids destructor of phenol identification |
| title | Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain |
| title_full | Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain |
| title_fullStr | Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain |
| title_full_unstemmed | Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain |
| title_short | Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain |
| title_sort | phenol oxidizing activity and fatty acid profile of brevibacillus centrosporus f14 strain |
| topic | brevibacillus centrosporus composition of fatty acids destructor of phenol identification |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2020/02/Gudzenko_1_20.pdf |
| work_keys_str_mv | AT tvgudzenko phenoloxidizingactivityandfattyacidprofileofbrevibacilluscentrosporusf14strain AT ovvoliuvach phenoloxidizingactivityandfattyacidprofileofbrevibacilluscentrosporusf14strain AT oggorshkova phenoloxidizingactivityandfattyacidprofileofbrevibacilluscentrosporusf14strain AT amostapchuk phenoloxidizingactivityandfattyacidprofileofbrevibacilluscentrosporusf14strain AT voivanytsia phenoloxidizingactivityandfattyacidprofileofbrevibacilluscentrosporusf14strain |