Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection
Avian influenza A (H7N9) virus infections frequently lead to acute respiratory distress syndrome and death in humans. The emergence of H7N9 virus infections is a serious public health threat. To identify virus–host interaction differences between the highly virulent H7N9 and pandemic influenza H1N1...
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MDPI AG
2022-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/5/781 |
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| author | Tzu-Hsuan Hsieh Ya-Jhu Lin Mei-Jen Hsioa Hsin-Ju Wang Lu-Ting Chen Shu-Li Yang Chung-Guei Huang |
| author_facet | Tzu-Hsuan Hsieh Ya-Jhu Lin Mei-Jen Hsioa Hsin-Ju Wang Lu-Ting Chen Shu-Li Yang Chung-Guei Huang |
| author_sort | Tzu-Hsuan Hsieh |
| collection | DOAJ |
| description | Avian influenza A (H7N9) virus infections frequently lead to acute respiratory distress syndrome and death in humans. The emergence of H7N9 virus infections is a serious public health threat. To identify virus–host interaction differences between the highly virulent H7N9 and pandemic influenza H1N1 (pdmH1N1), RNA sequencing was performed of normal human bronchial epithelial (NHBE) cells infected with either virus. The transcriptomic analysis of host cellular responses to viral infection enables the identification of potential cellular factors related to infection. Significantly different gene expression patterns were found between pdmH1N1- and H7N9-infected NHBE cells. In addition, the H7N9 virus infection induced strong immune responses, while cellular repair mechanisms were inhibited. The differential expression of specific factors observed between avian H7N9 and pdmH1N1 influenza virus strains can account for variations in disease pathogenicity. These findings provide a framework for future studies examining the molecular mechanisms underlying the pathogenicity of avian H7N9 virus. |
| first_indexed | 2024-03-09T20:44:22Z |
| format | Article |
| id | doaj.art-1306caea5d824d67b12022c025cc9a7b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T20:44:22Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1306caea5d824d67b12022c025cc9a7b2023-11-23T22:50:08ZengMDPI AGCells2073-44092022-02-0111578110.3390/cells11050781Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus InfectionTzu-Hsuan Hsieh0Ya-Jhu Lin1Mei-Jen Hsioa2Hsin-Ju Wang3Lu-Ting Chen4Shu-Li Yang5Chung-Guei Huang6Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanAvian influenza A (H7N9) virus infections frequently lead to acute respiratory distress syndrome and death in humans. The emergence of H7N9 virus infections is a serious public health threat. To identify virus–host interaction differences between the highly virulent H7N9 and pandemic influenza H1N1 (pdmH1N1), RNA sequencing was performed of normal human bronchial epithelial (NHBE) cells infected with either virus. The transcriptomic analysis of host cellular responses to viral infection enables the identification of potential cellular factors related to infection. Significantly different gene expression patterns were found between pdmH1N1- and H7N9-infected NHBE cells. In addition, the H7N9 virus infection induced strong immune responses, while cellular repair mechanisms were inhibited. The differential expression of specific factors observed between avian H7N9 and pdmH1N1 influenza virus strains can account for variations in disease pathogenicity. These findings provide a framework for future studies examining the molecular mechanisms underlying the pathogenicity of avian H7N9 virus.https://www.mdpi.com/2073-4409/11/5/781avian influenza A(H7N9) viruspandemic influenza H1N1 (pdmH1N1)transcriptome |
| spellingShingle | Tzu-Hsuan Hsieh Ya-Jhu Lin Mei-Jen Hsioa Hsin-Ju Wang Lu-Ting Chen Shu-Li Yang Chung-Guei Huang Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection Cells avian influenza A(H7N9) virus pandemic influenza H1N1 (pdmH1N1) transcriptome |
| title | Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection |
| title_full | Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection |
| title_fullStr | Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection |
| title_full_unstemmed | Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection |
| title_short | Transcriptome Differences in Normal Human Bronchial Epithelial Cells in Response to Influenza A pdmH1N1 or H7N9 Virus Infection |
| title_sort | transcriptome differences in normal human bronchial epithelial cells in response to influenza a pdmh1n1 or h7n9 virus infection |
| topic | avian influenza A(H7N9) virus pandemic influenza H1N1 (pdmH1N1) transcriptome |
| url | https://www.mdpi.com/2073-4409/11/5/781 |
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