Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes
Anthocyanins endowing strawberry fruit red color are regulated by the MYB-bHLH-WD40 complex. By analyzing the MYBs involved in the flavonoid biosynthesis in strawberry, we found that R2R3-FaMYB5 promoted the content of anthocyanin and proanthocyanidins in strawberry fruits. Yeast two-hybrid and BiFC...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1145670/full |
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| author | Maolan Yue Leiyu Jiang Nating Zhang Lianxi Zhang Yongqiang Liu Yuanxiu Lin Yuanxiu Lin Yunting Zhang Yunting Zhang Ya Luo Yong Zhang Yan Wang Yan Wang Mengyao Li Xiaorong Wang Xiaorong Wang Qing Chen Haoru Tang Haoru Tang |
| author_facet | Maolan Yue Leiyu Jiang Nating Zhang Lianxi Zhang Yongqiang Liu Yuanxiu Lin Yuanxiu Lin Yunting Zhang Yunting Zhang Ya Luo Yong Zhang Yan Wang Yan Wang Mengyao Li Xiaorong Wang Xiaorong Wang Qing Chen Haoru Tang Haoru Tang |
| author_sort | Maolan Yue |
| collection | DOAJ |
| description | Anthocyanins endowing strawberry fruit red color are regulated by the MYB-bHLH-WD40 complex. By analyzing the MYBs involved in the flavonoid biosynthesis in strawberry, we found that R2R3-FaMYB5 promoted the content of anthocyanin and proanthocyanidins in strawberry fruits. Yeast two-hybrid and BiFC assays confirmed that MBW complexes connected with flavonoid metabolism were FaMYB5/FaMYB10-FaEGL3 (bHLH)-FaLWD1/FaLWD1-like (WD40). Transient overexpression and qRT-PCR analysis revealed that disparate MBW models hold different patterns in the regulation of flavonoid biosynthesis in strawberry fruits. Compared with FaMYB10, FaMYB5 and its dominant complexes showed a more specific regulatory range on strawberry flavonoid biosynthetic pathway, while FaMYB10 was more extensive. In addition, the complexes involved in FaMYB5 facilitated PAs accumulation primarily through the LAR tributary while FaMYB10 mainly by the ANR branch. FaMYB9 and FaMYB11 tremendously elicited the accumulation of proanthocyanidins by up-regulating the expression levels of both LAR and ANR, and also affected anthocyanin metabolism by changing the ratio of Cy3G and Pg3G which were constituted as two major anthocyanin monomers in strawberries. Our study also illustrated that FaMYB5-FaEGL3-FaLWD1-like directly targeted the promoters of F3′H, LAR, and AHA10 thus committing to flavonoid accumulation. These results allow the specific members involved in the MBW complex to be deciphered and provide new insights into the regulatory mechanisms of anthocyanins and proanthocyanidins regulated by the MBW complex. |
| first_indexed | 2024-04-10T04:05:41Z |
| format | Article |
| id | doaj.art-1308796f862347cca2ec307c99a5c80b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T04:05:41Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1308796f862347cca2ec307c99a5c80b2023-03-13T05:33:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11456701145670Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexesMaolan Yue0Leiyu Jiang1Nating Zhang2Lianxi Zhang3Yongqiang Liu4Yuanxiu Lin5Yuanxiu Lin6Yunting Zhang7Yunting Zhang8Ya Luo9Yong Zhang10Yan Wang11Yan Wang12Mengyao Li13Xiaorong Wang14Xiaorong Wang15Qing Chen16Haoru Tang17Haoru Tang18Country College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology & Olericulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology & Olericulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology & Olericulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology & Olericulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCountry College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology & Olericulture, Sichuan Agricultural University, Chengdu, ChinaAnthocyanins endowing strawberry fruit red color are regulated by the MYB-bHLH-WD40 complex. By analyzing the MYBs involved in the flavonoid biosynthesis in strawberry, we found that R2R3-FaMYB5 promoted the content of anthocyanin and proanthocyanidins in strawberry fruits. Yeast two-hybrid and BiFC assays confirmed that MBW complexes connected with flavonoid metabolism were FaMYB5/FaMYB10-FaEGL3 (bHLH)-FaLWD1/FaLWD1-like (WD40). Transient overexpression and qRT-PCR analysis revealed that disparate MBW models hold different patterns in the regulation of flavonoid biosynthesis in strawberry fruits. Compared with FaMYB10, FaMYB5 and its dominant complexes showed a more specific regulatory range on strawberry flavonoid biosynthetic pathway, while FaMYB10 was more extensive. In addition, the complexes involved in FaMYB5 facilitated PAs accumulation primarily through the LAR tributary while FaMYB10 mainly by the ANR branch. FaMYB9 and FaMYB11 tremendously elicited the accumulation of proanthocyanidins by up-regulating the expression levels of both LAR and ANR, and also affected anthocyanin metabolism by changing the ratio of Cy3G and Pg3G which were constituted as two major anthocyanin monomers in strawberries. Our study also illustrated that FaMYB5-FaEGL3-FaLWD1-like directly targeted the promoters of F3′H, LAR, and AHA10 thus committing to flavonoid accumulation. These results allow the specific members involved in the MBW complex to be deciphered and provide new insights into the regulatory mechanisms of anthocyanins and proanthocyanidins regulated by the MBW complex.https://www.frontiersin.org/articles/10.3389/fpls.2023.1145670/fullanthocyaninproanthocyanidinsstrawberryMBW complexFaMYB5FaMYB10 |
| spellingShingle | Maolan Yue Leiyu Jiang Nating Zhang Lianxi Zhang Yongqiang Liu Yuanxiu Lin Yuanxiu Lin Yunting Zhang Yunting Zhang Ya Luo Yong Zhang Yan Wang Yan Wang Mengyao Li Xiaorong Wang Xiaorong Wang Qing Chen Haoru Tang Haoru Tang Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes Frontiers in Plant Science anthocyanin proanthocyanidins strawberry MBW complex FaMYB5 FaMYB10 |
| title | Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes |
| title_full | Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes |
| title_fullStr | Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes |
| title_full_unstemmed | Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes |
| title_short | Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes |
| title_sort | regulation of flavonoids in strawberry fruits by famyb5 famyb10 dominated myb bhlh wd40 ternary complexes |
| topic | anthocyanin proanthocyanidins strawberry MBW complex FaMYB5 FaMYB10 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1145670/full |
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