Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran
Background: One of the main causes of sexually transmitted diseases is group B β- hemolytic streptococci (GBS) multiplying in the genital tracts. Penicillin is the most common drug for the treatment of infections caused by these bacteria, but in patients suffering from Penicillin allergy, Erythromyc...
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| Format: | Article |
| Language: | English |
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Varastegan Institute for Medical Sciences
2015-05-01
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| Series: | Reports of Biochemistry and Molecular Biology |
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| Online Access: | http://rbmb.net/article-1-64-en.pdf |
| _version_ | 1818953107572260864 |
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| author | Masoumeh Malek-Jafarian Fatemeh-Sadat Hosseini Abodol-Reza Ahmadi |
| author_facet | Masoumeh Malek-Jafarian Fatemeh-Sadat Hosseini Abodol-Reza Ahmadi |
| author_sort | Masoumeh Malek-Jafarian |
| collection | DOAJ |
| description | Background: One of the main causes of sexually transmitted diseases is group B β- hemolytic streptococci (GBS) multiplying in the genital tracts. Penicillin is the most common drug for the treatment of infections caused by these bacteria, but in patients suffering from Penicillin allergy, Erythromycin and Clindamycin are used as alternative therapeutic drugs against GBS. Recently, resistance to these drugs has been reported more often. In this study, efforts have been made to determine the prevalence and antibiotic resistance of GBS.
Methods: Modified Christie Atkins Munch-Petersen (CAMP) test was conducted on over 2400 samples of urine and discharge taken from vagina, urethra and prostate. The drug sensitivity was performed by double disk sensitivity tests to Bacitracin, Trimethoprim, and Sulfamethoxazole and then the resistant samples were investigated by E-test to determine the minimal inhibitory concentrations (MICs) value.
Results: Twenty-three vaginal and 10 urethral discharge, 27urine and 6 prostatic secretion samples were GBS positive. The most symbiotic microorganisms with GBS were strains of Enterococci (90%), Staphylococcus saprophyticus (25%) and Candida albicans (6%). The disk diffusion method showed 18 cases with Penicillin resistance (MIC: 1.5 mg/ml).
Conclusion: Taken together, GBS carriers’ rate in this study was found 20.65% (8.24% men and 12.4% women). Furthermore, findings showed high-level resistance to Erythromycin and Clindamycin. |
| first_indexed | 2024-12-20T10:01:01Z |
| format | Article |
| id | doaj.art-1309aad75ab147ca8d106a345e485970 |
| institution | Directory Open Access Journal |
| issn | 2322-3480 2322-3480 |
| language | English |
| last_indexed | 2024-12-20T10:01:01Z |
| publishDate | 2015-05-01 |
| publisher | Varastegan Institute for Medical Sciences |
| record_format | Article |
| series | Reports of Biochemistry and Molecular Biology |
| spelling | doaj.art-1309aad75ab147ca8d106a345e4859702022-12-21T19:44:21ZengVarastegan Institute for Medical SciencesReports of Biochemistry and Molecular Biology2322-34802322-34802015-05-01328993Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, IranMasoumeh Malek-Jafarian0Fatemeh-Sadat Hosseini1Abodol-Reza Ahmadi2Department of Laboratory Medicine, School of Paramedicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Laboratory Medicine, School of Paramedicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Laboratory Medicine, School of Paramedicine, Mashhad University of Medical Sciences, Mashhad, IranBackground: One of the main causes of sexually transmitted diseases is group B β- hemolytic streptococci (GBS) multiplying in the genital tracts. Penicillin is the most common drug for the treatment of infections caused by these bacteria, but in patients suffering from Penicillin allergy, Erythromycin and Clindamycin are used as alternative therapeutic drugs against GBS. Recently, resistance to these drugs has been reported more often. In this study, efforts have been made to determine the prevalence and antibiotic resistance of GBS. Methods: Modified Christie Atkins Munch-Petersen (CAMP) test was conducted on over 2400 samples of urine and discharge taken from vagina, urethra and prostate. The drug sensitivity was performed by double disk sensitivity tests to Bacitracin, Trimethoprim, and Sulfamethoxazole and then the resistant samples were investigated by E-test to determine the minimal inhibitory concentrations (MICs) value. Results: Twenty-three vaginal and 10 urethral discharge, 27urine and 6 prostatic secretion samples were GBS positive. The most symbiotic microorganisms with GBS were strains of Enterococci (90%), Staphylococcus saprophyticus (25%) and Candida albicans (6%). The disk diffusion method showed 18 cases with Penicillin resistance (MIC: 1.5 mg/ml). Conclusion: Taken together, GBS carriers’ rate in this study was found 20.65% (8.24% men and 12.4% women). Furthermore, findings showed high-level resistance to Erythromycin and Clindamycin.http://rbmb.net/article-1-64-en.pdfAntibiotic resistanceGenitourinary systemMinimal inhibitory concentration (MIC)Streptococcus agalactiae |
| spellingShingle | Masoumeh Malek-Jafarian Fatemeh-Sadat Hosseini Abodol-Reza Ahmadi Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran Reports of Biochemistry and Molecular Biology Antibiotic resistance Genitourinary system Minimal inhibitory concentration (MIC) Streptococcus agalactiae |
| title | Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran |
| title_full | Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran |
| title_fullStr | Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran |
| title_full_unstemmed | Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran |
| title_short | Pattern of Infection and Antibiotic Activity among Streptococcus agalactiae Isolates from Adults in Mashhad, Iran |
| title_sort | pattern of infection and antibiotic activity among streptococcus agalactiae isolates from adults in mashhad iran |
| topic | Antibiotic resistance Genitourinary system Minimal inhibitory concentration (MIC) Streptococcus agalactiae |
| url | http://rbmb.net/article-1-64-en.pdf |
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