Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak
Phytochemical investigation of the aerial parts of <i>Artemisia heptapotamica</i> Poljak led to the isolation of ten known compounds, including four alkyl <i>p</i>-coumarates: octadecyl <i>trans</i>-<i>p</i>-coumarate (<b>1</b>), icosy <...
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/7/2908 |
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| author | Umit Mukatay Mamdouh Nabil Samy Bharathi Avula Kumar Katragunta Moldir Kemelbek Azhar Zhubanova Ikhlas A. Khan Samir Anis Ross |
| author_facet | Umit Mukatay Mamdouh Nabil Samy Bharathi Avula Kumar Katragunta Moldir Kemelbek Azhar Zhubanova Ikhlas A. Khan Samir Anis Ross |
| author_sort | Umit Mukatay |
| collection | DOAJ |
| description | Phytochemical investigation of the aerial parts of <i>Artemisia heptapotamica</i> Poljak led to the isolation of ten known compounds, including four alkyl <i>p</i>-coumarates: octadecyl <i>trans</i>-<i>p</i>-coumarate (<b>1</b>), icosy <i>trans</i>-<i>p</i>-coumarate (<b>2</b>), docosyl <i>trans</i>-<i>p</i>-coumarate (<b>3</b>), and tetracosyl <i>trans</i>-<i>p</i>-coumarate (<b>4</b>), one sesquiterpene lactone: santonin (<b>5</b>), four flavonoids; axillarin (<b>6</b>), quercetin 3-<i>O</i>-methyl ether (<b>7</b>), luteolin (<b>8</b>), and quercetin (<b>9</b>), and one phenolic acid derivative: <i>p</i>-coumaric acid (<b>10</b>). The structures of the isolated compounds were identified by various spectroscopic analyses. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth of <i>Candida albicans</i>, <i>C. neoformans</i>, <i>Aspergillus fumigatus</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRS), <i>E. coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumonia</i>, and Vancomycin-resistant <i>Enterococci</i> (VRE) at the tested concentrations ranging from 8 to 200 μg/mL. The identification and tentative characterization of the secondary metabolites were conducted using LC-QToF analysis. This method helps in the putative characterization of sesquiterpene lactones, flavonoids, coumarate derivatives, and aliphatic compounds. The developed method identified 43 compounds, of which the majority were sesquiterpene lactones, such as eudesmanolides, germacranolides, and guaianolide derivatives, followed by flavonoids. The proposed LC-QToF method helps develop dereplication strategies and understand the major class of chemicals before proceeding with the isolation of compounds. |
| first_indexed | 2024-03-11T05:30:22Z |
| format | Article |
| id | doaj.art-130a96fd08e94ec792aad6e61738e739 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T05:30:22Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-130a96fd08e94ec792aad6e61738e7392023-11-17T17:10:43ZengMDPI AGMolecules1420-30492023-03-01287290810.3390/molecules28072908Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> PoljakUmit Mukatay0Mamdouh Nabil Samy1Bharathi Avula2Kumar Katragunta3Moldir Kemelbek4Azhar Zhubanova5Ikhlas A. Khan6Samir Anis Ross7Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, KazakhstanNational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USADepartment of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, KazakhstanNational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USAPhytochemical investigation of the aerial parts of <i>Artemisia heptapotamica</i> Poljak led to the isolation of ten known compounds, including four alkyl <i>p</i>-coumarates: octadecyl <i>trans</i>-<i>p</i>-coumarate (<b>1</b>), icosy <i>trans</i>-<i>p</i>-coumarate (<b>2</b>), docosyl <i>trans</i>-<i>p</i>-coumarate (<b>3</b>), and tetracosyl <i>trans</i>-<i>p</i>-coumarate (<b>4</b>), one sesquiterpene lactone: santonin (<b>5</b>), four flavonoids; axillarin (<b>6</b>), quercetin 3-<i>O</i>-methyl ether (<b>7</b>), luteolin (<b>8</b>), and quercetin (<b>9</b>), and one phenolic acid derivative: <i>p</i>-coumaric acid (<b>10</b>). The structures of the isolated compounds were identified by various spectroscopic analyses. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth of <i>Candida albicans</i>, <i>C. neoformans</i>, <i>Aspergillus fumigatus</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRS), <i>E. coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumonia</i>, and Vancomycin-resistant <i>Enterococci</i> (VRE) at the tested concentrations ranging from 8 to 200 μg/mL. The identification and tentative characterization of the secondary metabolites were conducted using LC-QToF analysis. This method helps in the putative characterization of sesquiterpene lactones, flavonoids, coumarate derivatives, and aliphatic compounds. The developed method identified 43 compounds, of which the majority were sesquiterpene lactones, such as eudesmanolides, germacranolides, and guaianolide derivatives, followed by flavonoids. The proposed LC-QToF method helps develop dereplication strategies and understand the major class of chemicals before proceeding with the isolation of compounds.https://www.mdpi.com/1420-3049/28/7/2908Asteraceae<i>Artemisia heptapotamica</i>LC-QTpoFsesquiterpene lactonesflavonoidscoumarate derivatives |
| spellingShingle | Umit Mukatay Mamdouh Nabil Samy Bharathi Avula Kumar Katragunta Moldir Kemelbek Azhar Zhubanova Ikhlas A. Khan Samir Anis Ross Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak Molecules Asteraceae <i>Artemisia heptapotamica</i> LC-QTpoF sesquiterpene lactones flavonoids coumarate derivatives |
| title | Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak |
| title_full | Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak |
| title_fullStr | Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak |
| title_full_unstemmed | Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak |
| title_short | Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant <i>Artemisia heptapotamica</i> Poljak |
| title_sort | isolation and lc qtof characterization of secondary metabolites from an endemic plant i artemisia heptapotamica i poljak |
| topic | Asteraceae <i>Artemisia heptapotamica</i> LC-QTpoF sesquiterpene lactones flavonoids coumarate derivatives |
| url | https://www.mdpi.com/1420-3049/28/7/2908 |
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