Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide
Graphene oxide (GO), a chemically oxidized sheet of graphite, has been used as a conductive carbon carrier of microbes to boost various bioelectrochemical reactions. However, the types of microbes that can reduce GO have rarely been investigated. In this study, a strain of GO-reducing bacteria, name...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/349 |
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| author | Li Xie Naoko Yoshida Lingyu Meng |
| author_facet | Li Xie Naoko Yoshida Lingyu Meng |
| author_sort | Li Xie |
| collection | DOAJ |
| description | Graphene oxide (GO), a chemically oxidized sheet of graphite, has been used as a conductive carbon carrier of microbes to boost various bioelectrochemical reactions. However, the types of microbes that can reduce GO have rarely been investigated. In this study, a strain of GO-reducing bacteria, named NIT-SL11, which was obtained from a hydrogel of microbially reduced GO and anaerobic sludge that converts sewage to electricity, was phylogenically identified as a novel strain of <i>Geotalea uraniireducens</i>. Considering the current lack of information on the electrogenic ability of the bacterium and its physicochemical and chemotaxonomic characteristics, the polyphasic characterization of the <i>Geotalea uraniireducens</i> strain NIT-SL11 was performed. NIT-SL11 utilized various organic acids, such as lactate, benzoate, and formate, as electron donors and exhibited respiration using GO, electrodes, fumarate, and malate. The strain contained C16:1ω7c and C16:0 as the major fatty acids and MK-8 and 9 as the major respiratory quinones. The complete genome of NIT-SL11 was 4.7 Mbp in size with a G+C content of 60.9%, and it encoded 80 putative c-type cytochromes and 23 type IV pili-related proteins. The possible extracellular electron transfer (EET) pathways of the strain were the porin–cytochrome (Pcc) EET pathway and type IV pili-based pathway. |
| first_indexed | 2024-03-11T08:22:49Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T08:22:49Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-130b170ac53742478a0514069c3267dc2023-11-16T22:14:39ZengMDPI AGMicroorganisms2076-26072023-01-0111234910.3390/microorganisms11020349Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene OxideLi Xie0Naoko Yoshida1Lingyu Meng2Department of Civil Engineering, Nagoya Institute of Technology (Nitech), Nagoya 466-8555, JapanDepartment of Civil Engineering, Nagoya Institute of Technology (Nitech), Nagoya 466-8555, JapanDepartment of Civil Engineering, Nagoya Institute of Technology (Nitech), Nagoya 466-8555, JapanGraphene oxide (GO), a chemically oxidized sheet of graphite, has been used as a conductive carbon carrier of microbes to boost various bioelectrochemical reactions. However, the types of microbes that can reduce GO have rarely been investigated. In this study, a strain of GO-reducing bacteria, named NIT-SL11, which was obtained from a hydrogel of microbially reduced GO and anaerobic sludge that converts sewage to electricity, was phylogenically identified as a novel strain of <i>Geotalea uraniireducens</i>. Considering the current lack of information on the electrogenic ability of the bacterium and its physicochemical and chemotaxonomic characteristics, the polyphasic characterization of the <i>Geotalea uraniireducens</i> strain NIT-SL11 was performed. NIT-SL11 utilized various organic acids, such as lactate, benzoate, and formate, as electron donors and exhibited respiration using GO, electrodes, fumarate, and malate. The strain contained C16:1ω7c and C16:0 as the major fatty acids and MK-8 and 9 as the major respiratory quinones. The complete genome of NIT-SL11 was 4.7 Mbp in size with a G+C content of 60.9%, and it encoded 80 putative c-type cytochromes and 23 type IV pili-related proteins. The possible extracellular electron transfer (EET) pathways of the strain were the porin–cytochrome (Pcc) EET pathway and type IV pili-based pathway.https://www.mdpi.com/2076-2607/11/2/349<i>Geotalea</i>electrogenic bacteriacomplete genomegraphene oxide |
| spellingShingle | Li Xie Naoko Yoshida Lingyu Meng Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide Microorganisms <i>Geotalea</i> electrogenic bacteria complete genome graphene oxide |
| title | Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide |
| title_full | Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide |
| title_fullStr | Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide |
| title_full_unstemmed | Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide |
| title_short | Polyphasic Characterization of <i>Geotalea uranireducens</i> NIT-SL11 Newly Isolated from a Complex of Sewage Sludge and Microbially Reduced Graphene Oxide |
| title_sort | polyphasic characterization of i geotalea uranireducens i nit sl11 newly isolated from a complex of sewage sludge and microbially reduced graphene oxide |
| topic | <i>Geotalea</i> electrogenic bacteria complete genome graphene oxide |
| url | https://www.mdpi.com/2076-2607/11/2/349 |
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