STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION
The aim of our paper was to make a series of experiments in order to determine the concentration at which four cryoprotectants (DMSO, sucrose, Ficoll 70 and galactose) singly and in pairs would vitrify on plunging into liquid nitrogen and remain vitreous when thawed in water bath. As penetrating cry...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1727 |
| _version_ | 1827289322496196608 |
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| author | ADA TELEA N. PĂCALĂ ALEXANDRA IVAN V. CARABĂ B. KORBULY |
| author_facet | ADA TELEA N. PĂCALĂ ALEXANDRA IVAN V. CARABĂ B. KORBULY |
| author_sort | ADA TELEA |
| collection | DOAJ |
| description | The aim of our paper was to make a series of experiments in order to determine the
concentration at which four cryoprotectants (DMSO, sucrose, Ficoll 70 and
galactose) singly and in pairs would vitrify on plunging into liquid nitrogen and
remain vitreous when thawed in water bath. As penetrating cryoprotector we used
DMSO (MW=78.13 Da, Sigma D5879) and as nonpenetrating cryoprotectors we
used sucrose (MW=342.3 Da, Sigma S7903), Ficoll 70 (MW= 60,000-80,000 Da,
Sigma F4375) and Galactose (MW = 180,16 Da; Sigma G 6152). For DMSO there
were tested concentrations from 1M to 6.5M, with concentrations step of 0.5M. For
the nonpenetrating cryoprotectors there were tested concentrations of 5%, 10%,
15% and 20%. There were a total number of 168 solutions tested. The solutions
vitrification ability on freezing was tested by direct plunging into liquid nitrogen at -
196°C. Three thawing temperatures were tested 20°C, 25°C and 37°C. The
concentration at which DMSO solutions passed into vitreous state was 5M, but at
thawing none of them remained vitreous at thawing. When pairs of cryoprotectors
were tested 67 solutions vitrified at freezing (23 for DMSO-sucrose, 23 for DMSOFicoll 70 and 21 for DMSO-galactose) and 26 of them remained vitreous at
thawing. The DMSO and galacose pair give the best results on thawing (11 solution
remained vitreous on warming) at 37°C. |
| first_indexed | 2024-04-24T11:47:39Z |
| format | Article |
| id | doaj.art-130b77d890cb4036bf7761dcfd6e900f |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:47:39Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-130b77d890cb4036bf7761dcfd6e900f2024-04-09T08:50:27ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014111621621727STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATIONADA TELEA0N. PĂCALĂ1ALEXANDRA IVAN2V. CARABĂ3B. KORBULY4Faculty of Animal Sciences and Biotechnologies, Timisoara, Romania Faculty of Animal Sciences and Biotechnologies, Timisoara, Romania Faculty of Animal Sciences and Biotechnologies, Timisoara, Romania Faculty of Animal Sciences and Biotechnologies, Timisoara, Romania Faculty of Animal Sciences and Biotechnologies, Timisoara, Romania The aim of our paper was to make a series of experiments in order to determine the concentration at which four cryoprotectants (DMSO, sucrose, Ficoll 70 and galactose) singly and in pairs would vitrify on plunging into liquid nitrogen and remain vitreous when thawed in water bath. As penetrating cryoprotector we used DMSO (MW=78.13 Da, Sigma D5879) and as nonpenetrating cryoprotectors we used sucrose (MW=342.3 Da, Sigma S7903), Ficoll 70 (MW= 60,000-80,000 Da, Sigma F4375) and Galactose (MW = 180,16 Da; Sigma G 6152). For DMSO there were tested concentrations from 1M to 6.5M, with concentrations step of 0.5M. For the nonpenetrating cryoprotectors there were tested concentrations of 5%, 10%, 15% and 20%. There were a total number of 168 solutions tested. The solutions vitrification ability on freezing was tested by direct plunging into liquid nitrogen at - 196°C. Three thawing temperatures were tested 20°C, 25°C and 37°C. The concentration at which DMSO solutions passed into vitreous state was 5M, but at thawing none of them remained vitreous at thawing. When pairs of cryoprotectors were tested 67 solutions vitrified at freezing (23 for DMSO-sucrose, 23 for DMSOFicoll 70 and 21 for DMSO-galactose) and 26 of them remained vitreous at thawing. The DMSO and galacose pair give the best results on thawing (11 solution remained vitreous on warming) at 37°C.https://spasb.ro/index.php/public_html/article/view/1727crioprotectorsvitrificationdmsosucroseficoll 70galactose |
| spellingShingle | ADA TELEA N. PĂCALĂ ALEXANDRA IVAN V. CARABĂ B. KORBULY STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION Scientific Papers Animal Science and Biotechnologies crioprotectors vitrification dmso sucrose ficoll 70 galactose |
| title | STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION |
| title_full | STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION |
| title_fullStr | STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION |
| title_full_unstemmed | STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION |
| title_short | STUDIES REGARDING THE CRIOPROTECTIVE PROPRIETIES OF THE VITRIFICATION MEDIA, WITH DMSO, SUCROSE, FICOLL 70 AND GALACTOSE USED IN EMBRYO CRYOPRESERVATION |
| title_sort | studies regarding the crioprotective proprieties of the vitrification media with dmso sucrose ficoll 70 and galactose used in embryo cryopreservation |
| topic | crioprotectors vitrification dmso sucrose ficoll 70 galactose |
| url | https://spasb.ro/index.php/public_html/article/view/1727 |
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