Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD
Abstract Introduction Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Genes and Environment |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41021-018-0095-2 |
| _version_ | 1818765966709882880 |
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| author | Kazuaki Kawai Hiroshi Kasai Yun-Shan Li Yuya Kawasaki Shintaro Watanabe Masanori Ohta Toru Honda Hiroshi Yamato |
| author_facet | Kazuaki Kawai Hiroshi Kasai Yun-Shan Li Yuya Kawasaki Shintaro Watanabe Masanori Ohta Toru Honda Hiroshi Yamato |
| author_sort | Kazuaki Kawai |
| collection | DOAJ |
| description | Abstract Introduction Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) as an oxidative stress marker in saliva, by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD). Findings The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The average level of 8-OHGua in saliva was 3.80 ng/mL in ordinary, non-smoking subjects. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers. Conclusions Salivary 8-OHGua may be a useful noninvasive and promising oxidative stress biomarker. |
| first_indexed | 2024-12-18T08:26:29Z |
| format | Article |
| id | doaj.art-13109966bd6a4882be245f108c2714ad |
| institution | Directory Open Access Journal |
| issn | 1880-7062 |
| language | English |
| last_indexed | 2024-12-18T08:26:29Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Genes and Environment |
| spelling | doaj.art-13109966bd6a4882be245f108c2714ad2022-12-21T21:14:35ZengBMCGenes and Environment1880-70622018-04-014011410.1186/s41021-018-0095-2Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECDKazuaki Kawai0Hiroshi Kasai1Yun-Shan Li2Yuya Kawasaki3Shintaro Watanabe4Masanori Ohta5Toru Honda6Hiroshi Yamato7Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Health Development, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Health Development, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthDepartment of Health Development, Institute of Industrial Ecological Sciences, University of Occupational and Environmental HealthAbstract Introduction Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) as an oxidative stress marker in saliva, by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD). Findings The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The average level of 8-OHGua in saliva was 3.80 ng/mL in ordinary, non-smoking subjects. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers. Conclusions Salivary 8-OHGua may be a useful noninvasive and promising oxidative stress biomarker.http://link.springer.com/article/10.1186/s41021-018-0095-2Oxidative stressBiomarker8-hydroxyguanineSalivaHPLC-ECD |
| spellingShingle | Kazuaki Kawai Hiroshi Kasai Yun-Shan Li Yuya Kawasaki Shintaro Watanabe Masanori Ohta Toru Honda Hiroshi Yamato Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD Genes and Environment Oxidative stress Biomarker 8-hydroxyguanine Saliva HPLC-ECD |
| title | Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD |
| title_full | Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD |
| title_fullStr | Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD |
| title_full_unstemmed | Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD |
| title_short | Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD |
| title_sort | measurement of 8 hydroxyguanine as an oxidative stress biomarker in saliva by hplc ecd |
| topic | Oxidative stress Biomarker 8-hydroxyguanine Saliva HPLC-ECD |
| url | http://link.springer.com/article/10.1186/s41021-018-0095-2 |
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