Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene
Amphibians inhabit a wide variety of habitats and environments, such as arboreal, terrestrial and freshwater aquatic ecosystems, and are considered among the vertebrate classes to exhibit one of the most diverse distributions. Their life histories, behaviour and diversity play vital roles in assessi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722004815 |
| _version_ | 1828730226991955968 |
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| author | Abdulwahed Fahad Alrefaei |
| author_facet | Abdulwahed Fahad Alrefaei |
| author_sort | Abdulwahed Fahad Alrefaei |
| collection | DOAJ |
| description | Amphibians inhabit a wide variety of habitats and environments, such as arboreal, terrestrial and freshwater aquatic ecosystems, and are considered among the vertebrate classes to exhibit one of the most diverse distributions. Their life histories, behaviour and diversity play vital roles in assessing environmental and evolutionary theories. The mitochondrial 16S rRNA gene sequence analysis has been widely used in molecular taxonomy and is frequently used in systematic analyses of families and genera. This study examines taxonomic relationships among 18 specimens of the Arabian toad (Sclerophrys arabica) and the Dhofar toad (Bufo dhufarensis) from Al Madinah Province, Saudi Arabia by estimating phylogenetic relationships using the mitochondrial 16S ribosomal RNA gene (16S rRNA). Results indicate the presence of four new lineages of toads among the samples in this study. The generated phylogenetic tree grouped all samples of Arabian toad in one clade, while the Dhofar toad samples were divided into three subclades. This is the first study to be reported the genetic diversity of Al Madinah Province toads. |
| first_indexed | 2024-04-12T17:27:00Z |
| format | Article |
| id | doaj.art-1310d6f7429840ceab1b7caa2f930ea3 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T17:27:00Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-1310d6f7429840ceab1b7caa2f930ea32022-12-22T03:23:16ZengElsevierJournal of King Saud University: Science1018-36472022-11-01348102300Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA geneAbdulwahed Fahad Alrefaei0Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaAmphibians inhabit a wide variety of habitats and environments, such as arboreal, terrestrial and freshwater aquatic ecosystems, and are considered among the vertebrate classes to exhibit one of the most diverse distributions. Their life histories, behaviour and diversity play vital roles in assessing environmental and evolutionary theories. The mitochondrial 16S rRNA gene sequence analysis has been widely used in molecular taxonomy and is frequently used in systematic analyses of families and genera. This study examines taxonomic relationships among 18 specimens of the Arabian toad (Sclerophrys arabica) and the Dhofar toad (Bufo dhufarensis) from Al Madinah Province, Saudi Arabia by estimating phylogenetic relationships using the mitochondrial 16S ribosomal RNA gene (16S rRNA). Results indicate the presence of four new lineages of toads among the samples in this study. The generated phylogenetic tree grouped all samples of Arabian toad in one clade, while the Dhofar toad samples were divided into three subclades. This is the first study to be reported the genetic diversity of Al Madinah Province toads.http://www.sciencedirect.com/science/article/pii/S1018364722004815Toads16S rRNADhofar toadArabian toadPhylogenetic treePCR |
| spellingShingle | Abdulwahed Fahad Alrefaei Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene Journal of King Saud University: Science Toads 16S rRNA Dhofar toad Arabian toad Phylogenetic tree PCR |
| title | Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene |
| title_full | Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene |
| title_fullStr | Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene |
| title_full_unstemmed | Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene |
| title_short | Molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16S ribosomal RNA gene |
| title_sort | molecular phylogenetic investigation of toads based on sequencing of the mitochondrial 16s ribosomal rna gene |
| topic | Toads 16S rRNA Dhofar toad Arabian toad Phylogenetic tree PCR |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722004815 |
| work_keys_str_mv | AT abdulwahedfahadalrefaei molecularphylogeneticinvestigationoftoadsbasedonsequencingofthemitochondrial16sribosomalrnagene |