Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors
Developing label-free immunosensors to detect ovarian cancer (OC) by cancer antigen (CA125) is essential to improving diagnosis and protecting women from life-threatening diseases. Four types of carbon nanomaterials, such as multi-wall carbon nanotubes (MWCNTs), vapor-grown carbon fiber (VGCFs), gra...
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MDPI AG
2023-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/3/1131 |
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| author | Nattharika Runprapan Fu-Ming Wang Alagar Ramar Chiou-Chung Yuan |
| author_facet | Nattharika Runprapan Fu-Ming Wang Alagar Ramar Chiou-Chung Yuan |
| author_sort | Nattharika Runprapan |
| collection | DOAJ |
| description | Developing label-free immunosensors to detect ovarian cancer (OC) by cancer antigen (CA125) is essential to improving diagnosis and protecting women from life-threatening diseases. Four types of carbon nanomaterials, such as multi-wall carbon nanotubes (MWCNTs), vapor-grown carbon fiber (VGCFs), graphite KS4, and carbon black super P (SP), have been treated with acids to prepare a carbon nanomaterial/gold (Au) nanocomposite. The AuNPs@carbon nanocomposite was electrochemically deposited on a glassy carbon electrode (GCE) to serve as a substrate to fabricate a label-free immunosensor for the detection of CA125. Among the four AuNPs@carbon composite, the AuNPs@MWCNTs-based sensor exhibited a high sensitivity of 0.001 µg/mL for the biomarker CA125 through the square wave voltammetry (SWV) technique. The high conductivity and surface area of MWCNTs supported the immobilization of AuNPs. Moreover, the carboxylic (COO-) functional groups in MWCNT improved to a higher quantity after the acid treatment, which served as an excellent support for the fabrication of electrochemical biosensors. The present method aims to explore an environmentally friendly synthesis of a layer-by-layer (LBL) assembly of AuNPs@carbon nanomaterials electrochemical immunoassay to CA125 in a clinical diagnosis at a low cost and proved feasible for point-of-care diagnosis. |
| first_indexed | 2024-03-11T09:25:51Z |
| format | Article |
| id | doaj.art-13115cdcf6054818936f968a1cf13f08 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T09:25:51Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-13115cdcf6054818936f968a1cf13f082023-11-16T17:56:37ZengMDPI AGSensors1424-82202023-01-01233113110.3390/s23031131Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical ImmunosensorsNattharika Runprapan0Fu-Ming Wang1Alagar Ramar2Chiou-Chung Yuan3Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, TaiwanGraduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, TaiwanGraduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, TaiwanDepartment of Obstetrics and Gynecology, Cheng Hsin General Hospital, Taipei 112, TaiwanDeveloping label-free immunosensors to detect ovarian cancer (OC) by cancer antigen (CA125) is essential to improving diagnosis and protecting women from life-threatening diseases. Four types of carbon nanomaterials, such as multi-wall carbon nanotubes (MWCNTs), vapor-grown carbon fiber (VGCFs), graphite KS4, and carbon black super P (SP), have been treated with acids to prepare a carbon nanomaterial/gold (Au) nanocomposite. The AuNPs@carbon nanocomposite was electrochemically deposited on a glassy carbon electrode (GCE) to serve as a substrate to fabricate a label-free immunosensor for the detection of CA125. Among the four AuNPs@carbon composite, the AuNPs@MWCNTs-based sensor exhibited a high sensitivity of 0.001 µg/mL for the biomarker CA125 through the square wave voltammetry (SWV) technique. The high conductivity and surface area of MWCNTs supported the immobilization of AuNPs. Moreover, the carboxylic (COO-) functional groups in MWCNT improved to a higher quantity after the acid treatment, which served as an excellent support for the fabrication of electrochemical biosensors. The present method aims to explore an environmentally friendly synthesis of a layer-by-layer (LBL) assembly of AuNPs@carbon nanomaterials electrochemical immunoassay to CA125 in a clinical diagnosis at a low cost and proved feasible for point-of-care diagnosis.https://www.mdpi.com/1424-8220/23/3/1131CA125in situ reductiongold nanoparticles (AuNPs)label-free immunosensormulti-wall carbon nanotubes (MWCNTs) |
| spellingShingle | Nattharika Runprapan Fu-Ming Wang Alagar Ramar Chiou-Chung Yuan Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors Sensors CA125 in situ reduction gold nanoparticles (AuNPs) label-free immunosensor multi-wall carbon nanotubes (MWCNTs) |
| title | Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors |
| title_full | Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors |
| title_fullStr | Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors |
| title_full_unstemmed | Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors |
| title_short | Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors |
| title_sort | role of defects of carbon nanomaterials in the detection of ovarian cancer cells in label free electrochemical immunosensors |
| topic | CA125 in situ reduction gold nanoparticles (AuNPs) label-free immunosensor multi-wall carbon nanotubes (MWCNTs) |
| url | https://www.mdpi.com/1424-8220/23/3/1131 |
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