Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice
Currently used <i>Brucella</i> vaccines, <i>Brucella abortus</i> strain 19 and RB51, comprises of live attenuated <i>Brucella</i> strains and prevent infection in animals. However, these vaccines pose potential risks to recipient animals such as attenuation revers...
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MDPI AG
2020-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/2/152 |
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| author | Sonal Gupta Surender Mohan Vikas Kumar Somani Somya Aggarwal Rakesh Bhatnagar |
| author_facet | Sonal Gupta Surender Mohan Vikas Kumar Somani Somya Aggarwal Rakesh Bhatnagar |
| author_sort | Sonal Gupta |
| collection | DOAJ |
| description | Currently used <i>Brucella</i> vaccines, <i>Brucella abortus</i> strain 19 and RB51, comprises of live attenuated <i>Brucella</i> strains and prevent infection in animals. However, these vaccines pose potential risks to recipient animals such as attenuation reversal and virulence in susceptible hosts on administration. In this context, recombinant subunit vaccines emerge as a safe and competent alternative in combating the disease. In this study, we formulated a divalent recombinant vaccine consisting of Omp25 and L7/L12 of <i>B. abortus</i> and evaluated vaccine potential individually as well as in combination. Sera obtained from divalent vaccine (Omp25+L7/L12) immunized mice group exhibited enhanced IgG titers against both components and indicated specificity upon immunoblotting reiterating its authenticity. Further, the IgG1/IgG2a ratio obtained against each antigen predicted a predominant Th2 immune response in the Omp25+L7/L12 immunized mice group. Upon infection with virulent <i>B. abortus</i> 544, Omp25+L7/L12 infected mice exhibited superior Log10 protection compared to individual vaccines. Consequently, this study recommends that simultaneous immunization of Omp25 and L7/L12 as a divalent vaccine complements and triggers a Th2 mediated immune response in mice competent of providing protection against brucellosis. |
| first_indexed | 2024-04-11T22:32:37Z |
| format | Article |
| id | doaj.art-13128ff3ef444adf813338491a140e2f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T22:32:37Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-13128ff3ef444adf813338491a140e2f2022-12-22T03:59:18ZengMDPI AGPathogens2076-08172020-02-019215210.3390/pathogens9020152pathogens9020152Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in MiceSonal Gupta0Surender Mohan1Vikas Kumar Somani2Somya Aggarwal3Rakesh Bhatnagar4Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaCurrently used <i>Brucella</i> vaccines, <i>Brucella abortus</i> strain 19 and RB51, comprises of live attenuated <i>Brucella</i> strains and prevent infection in animals. However, these vaccines pose potential risks to recipient animals such as attenuation reversal and virulence in susceptible hosts on administration. In this context, recombinant subunit vaccines emerge as a safe and competent alternative in combating the disease. In this study, we formulated a divalent recombinant vaccine consisting of Omp25 and L7/L12 of <i>B. abortus</i> and evaluated vaccine potential individually as well as in combination. Sera obtained from divalent vaccine (Omp25+L7/L12) immunized mice group exhibited enhanced IgG titers against both components and indicated specificity upon immunoblotting reiterating its authenticity. Further, the IgG1/IgG2a ratio obtained against each antigen predicted a predominant Th2 immune response in the Omp25+L7/L12 immunized mice group. Upon infection with virulent <i>B. abortus</i> 544, Omp25+L7/L12 infected mice exhibited superior Log10 protection compared to individual vaccines. Consequently, this study recommends that simultaneous immunization of Omp25 and L7/L12 as a divalent vaccine complements and triggers a Th2 mediated immune response in mice competent of providing protection against brucellosis.https://www.mdpi.com/2076-0817/9/2/152recombinant vaccinedivalent vaccinebrucellosisomp25l7/l12<i>brucella abortus</i> 544 |
| spellingShingle | Sonal Gupta Surender Mohan Vikas Kumar Somani Somya Aggarwal Rakesh Bhatnagar Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice Pathogens recombinant vaccine divalent vaccine brucellosis omp25 l7/l12 <i>brucella abortus</i> 544 |
| title | Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice |
| title_full | Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice |
| title_fullStr | Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice |
| title_full_unstemmed | Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice |
| title_short | Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice |
| title_sort | simultaneous immunization with omp25 and l7 l12 provides protection against brucellosis in mice |
| topic | recombinant vaccine divalent vaccine brucellosis omp25 l7/l12 <i>brucella abortus</i> 544 |
| url | https://www.mdpi.com/2076-0817/9/2/152 |
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