Commercial scale genetic transformation of mature seed embryo explants in maize

Commercial scale genetic transformation of mature seed embryo explants in maize

A novel, efficient maize genetic transformation system was developed using Agrobacterium-mediated transformation of embryo explants from mature seeds. Seeds from field grown plants were sterilized and crushed to isolate embryo explants consisting of the coleoptile, leaf primordia, and shoot apical m...

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Bibliographic Details
Main Authors: Xudong Ye, Ashok Shrawat, Edward Williams, Anatoly Rivlin, Zarir Vaghchhipawala, Lorena Moeller, Jennifer Kumpf, Shubha Subbarao, Brian Martinell, Charles Armstrong, M. Annie Saltarikos, David Somers, Yurong Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Plant Science
Subjects:
Zea mays
maize transformation
seed embryo explants (SEEs)
meristem
Agrobacterium
organogenesis
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.1056190/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2022.1056190/full

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