Revisiting the Resazurin-Based Sensing of Cellular Viability: Widening the Application Horizon

Revisiting the Resazurin-Based Sensing of Cellular Viability: Widening the Application Horizon

Since 1991, the NAD(P)H-aided conversion of resazurin to fluorescent resorufin has been widely used to measure viability based on the metabolic activity in mammalian cell culture and primary cells. However, different research groups have used divergent assay protocols, scarcely reporting the systema...

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Bibliographic Details
Main Authors: Darja Lavogina, Helen Lust, Maris-Johanna Tahk, Tõnis Laasfeld, Hans Vellama, Naila Nasirova, Markus Vardja, Kattri-Liis Eskla, Andres Salumets, Ago Rinken, Jana Jaal
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Biosensors
Subjects:
resazurin
Alamar Blue
resorufin
fluorescence
absorbance
cell viability
Online Access:https://www.mdpi.com/2079-6374/12/4/196

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