Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling
Onion peels are often discarded, representing an unlimited amount of food by-products; however, they are a valuable source of bioactive phenolics. Thus, we utilized UPLC-MS/MS to analyze the metabolomic profiles of red (RO) and yellow (YO) onion peel extracts. The cytotoxic (SRB assay), anti-inflamm...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/10/1379 |
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| author | Rafik Mounir Walaa A. Alshareef Eman A. El Gebaly Alaadin E. El-Haddad Abdallah M. Said Ahmed Osama G. Mohamed Eman T. Enan Shaimaa Mosallam Ashootosh Tripathi Heba Mohammed Refat M. Selim Sarah I. Bukhari Rihaf Alfaraj Ghada M. Ragab Amira A. El-Gazar Soad Z. El-Emam |
| author_facet | Rafik Mounir Walaa A. Alshareef Eman A. El Gebaly Alaadin E. El-Haddad Abdallah M. Said Ahmed Osama G. Mohamed Eman T. Enan Shaimaa Mosallam Ashootosh Tripathi Heba Mohammed Refat M. Selim Sarah I. Bukhari Rihaf Alfaraj Ghada M. Ragab Amira A. El-Gazar Soad Z. El-Emam |
| author_sort | Rafik Mounir |
| collection | DOAJ |
| description | Onion peels are often discarded, representing an unlimited amount of food by-products; however, they are a valuable source of bioactive phenolics. Thus, we utilized UPLC-MS/MS to analyze the metabolomic profiles of red (RO) and yellow (YO) onion peel extracts. The cytotoxic (SRB assay), anti-inflammatory (Griess assay), and antimicrobial (sensitivity test, MIC, antibiofilm, and SP-SDS tests) properties were assessed in vitro. Additionally, histological analysis, immunohistochemistry, and ELISA tests were conducted to investigate the healing potential in excisional skin wound injury and <i>Candida albicans</i> infection in vivo. RO extract demonstrated antibacterial activity, limited skin infection with <i>C. albicans</i>, and improved the skin’s appearance due to the abundance of quercetin and anthocyanin derivatives. Both extracts reduced lipopolysaccharide-induced nitric oxide release in vitro and showed a negligible cytotoxic effect on MCF-7 and HT29 cells. When extracts were tested in vivo for their ability to promote tissue regeneration, it was found that YO peel extract had the greatest impact. Further biochemical analysis revealed that YO extract suppressed NLRP3/caspase-1 signaling and decreased inflammatory cytokines. Furthermore, YO extract decreased Notch-1 levels and boosted VEGF-mediated angiogenesis. Our findings imply that onion peel extract can effectively treat wounds by reducing microbial infection, reducing inflammation, and promoting tissue regeneration. |
| first_indexed | 2024-03-10T20:58:22Z |
| format | Article |
| id | doaj.art-1315ac7ace52458c88ed32ecb654c2df |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T20:58:22Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-1315ac7ace52458c88ed32ecb654c2df2023-11-19T17:41:39ZengMDPI AGPharmaceuticals1424-82472023-09-011610137910.3390/ph16101379Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 SignalingRafik Mounir0Walaa A. Alshareef1Eman A. El Gebaly2Alaadin E. El-Haddad3Abdallah M. Said Ahmed4Osama G. Mohamed5Eman T. Enan6Shaimaa Mosallam7Ashootosh Tripathi8Heba Mohammed Refat M. Selim9Sarah I. Bukhari10Rihaf Alfaraj11Ghada M. Ragab12Amira A. El-Gazar13Soad Z. El-Emam14Pharmacognosy Department, Faculty of Pharmacy, Misr University for Science and Technology, Giza 12585, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptPharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptPharmacology and Toxicology Department, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr el Aini St., Cairo 11562, EgyptDepartment of Pathology, Faculty of Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptNatural Products Discovery Core, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USADepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Al-Maarefa University, Diriyah, Riyadh 13713, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaPharmacology and Toxicology Department, Faculty of Pharmacy, Misr University for Science and Technology, Giza 12585, EgyptPharmacology and Toxicology Department, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptPharmacology and Toxicology Department, Faculty of Pharmacy, October 6 University, Giza 12585, EgyptOnion peels are often discarded, representing an unlimited amount of food by-products; however, they are a valuable source of bioactive phenolics. Thus, we utilized UPLC-MS/MS to analyze the metabolomic profiles of red (RO) and yellow (YO) onion peel extracts. The cytotoxic (SRB assay), anti-inflammatory (Griess assay), and antimicrobial (sensitivity test, MIC, antibiofilm, and SP-SDS tests) properties were assessed in vitro. Additionally, histological analysis, immunohistochemistry, and ELISA tests were conducted to investigate the healing potential in excisional skin wound injury and <i>Candida albicans</i> infection in vivo. RO extract demonstrated antibacterial activity, limited skin infection with <i>C. albicans</i>, and improved the skin’s appearance due to the abundance of quercetin and anthocyanin derivatives. Both extracts reduced lipopolysaccharide-induced nitric oxide release in vitro and showed a negligible cytotoxic effect on MCF-7 and HT29 cells. When extracts were tested in vivo for their ability to promote tissue regeneration, it was found that YO peel extract had the greatest impact. Further biochemical analysis revealed that YO extract suppressed NLRP3/caspase-1 signaling and decreased inflammatory cytokines. Furthermore, YO extract decreased Notch-1 levels and boosted VEGF-mediated angiogenesis. Our findings imply that onion peel extract can effectively treat wounds by reducing microbial infection, reducing inflammation, and promoting tissue regeneration.https://www.mdpi.com/1424-8247/16/10/1379dietary flavonoidsanthocyaninsquercetintissue regenerationangiogenesisVEGF |
| spellingShingle | Rafik Mounir Walaa A. Alshareef Eman A. El Gebaly Alaadin E. El-Haddad Abdallah M. Said Ahmed Osama G. Mohamed Eman T. Enan Shaimaa Mosallam Ashootosh Tripathi Heba Mohammed Refat M. Selim Sarah I. Bukhari Rihaf Alfaraj Ghada M. Ragab Amira A. El-Gazar Soad Z. El-Emam Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling Pharmaceuticals dietary flavonoids anthocyanins quercetin tissue regeneration angiogenesis VEGF |
| title | Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling |
| title_full | Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling |
| title_fullStr | Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling |
| title_full_unstemmed | Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling |
| title_short | Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling |
| title_sort | unlocking the power of onion peel extracts antimicrobial and anti inflammatory effects improve wound healing through repressing notch 1 nlrp3 caspase 1 signaling |
| topic | dietary flavonoids anthocyanins quercetin tissue regeneration angiogenesis VEGF |
| url | https://www.mdpi.com/1424-8247/16/10/1379 |
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