DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION
Chitooligosaccharide (COS) , which is a derivative product from chitosan, has recently been used as a functional food because it has antimicrobial, antifungal, and antitumor properties. The salt content in chitooligosaccharide is one of the main problems in application as functional food or pharmace...
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| Format: | Article |
| Language: | English |
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Kementerian Kelautan dan Perikanan
2014-12-01
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| Series: | Squalen |
| Subjects: | |
| Online Access: | http://bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/110 |
| _version_ | 1819072784250175488 |
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| author | Pujoyuwono Martosuyono Asri Pratitis Alexander Prasetya Elisabeth Kartika Prabawati |
| author_facet | Pujoyuwono Martosuyono Asri Pratitis Alexander Prasetya Elisabeth Kartika Prabawati |
| author_sort | Pujoyuwono Martosuyono |
| collection | DOAJ |
| description | Chitooligosaccharide (COS) , which is a derivative product from chitosan, has recently been used as a functional food because it has antimicrobial, antifungal, and antitumor properties. The salt content in chitooligosaccharide is one of the main problems in application as functional food or pharmaceutical medicine. The aim of this study was to remove salt from COS with two desalting techniques and determine the variation of COSs in the product. The desalting technique used were dialysis with 10kD Molecular Weight Cut Off (MWCO) and gel filtration chromatography HiPrep 26/10 desalting with G-25 Superfine Sephadex as stationary phase in the column. In order to detect the presence of COS, Thin Layer Chromatography (TLC) method was used, followed by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI–TOF–MS) to detect low concentration of COS. Qualitative and quantitative analysis of salt presence were identified using silver nitrate and Volhard method respectively. Ash content was measured using gravimetric method. Results showed those dialysis and gel filtration chromatographies were successfully remove the most of the salt from COS with efficiency of desalting up to 100%. However, the best desalting technique was gel filtration chromatography HiPrep 26/10 which has more complete COS with various degrees of polymerization present in the result. |
| first_indexed | 2024-12-21T17:43:13Z |
| format | Article |
| id | doaj.art-1315d3f0438b4cc3ba3444a879d5b2c1 |
| institution | Directory Open Access Journal |
| issn | 2089-5690 2406-9272 |
| language | English |
| last_indexed | 2024-12-21T17:43:13Z |
| publishDate | 2014-12-01 |
| publisher | Kementerian Kelautan dan Perikanan |
| record_format | Article |
| series | Squalen |
| spelling | doaj.art-1315d3f0438b4cc3ba3444a879d5b2c12022-12-21T18:55:34ZengKementerian Kelautan dan PerikananSqualen2089-56902406-92722014-12-019312713610.15578/squalen.v9i3.11082DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATIONPujoyuwono Martosuyono0Asri Pratitis1Alexander Prasetya2Elisabeth Kartika Prabawati3Research and Development Center for Marine and Fisheries Product Processing and BiotechnologyResearch and Development Center for Marine and Fisheries Product Processing and BiotechnologyDepartment of Food Technology, Swiss German University, EduTown BSD City, Tangerang, IndonesiaDepartment of Food Technology, Swiss German University, EduTown BSD City, Tangerang, IndonesiaChitooligosaccharide (COS) , which is a derivative product from chitosan, has recently been used as a functional food because it has antimicrobial, antifungal, and antitumor properties. The salt content in chitooligosaccharide is one of the main problems in application as functional food or pharmaceutical medicine. The aim of this study was to remove salt from COS with two desalting techniques and determine the variation of COSs in the product. The desalting technique used were dialysis with 10kD Molecular Weight Cut Off (MWCO) and gel filtration chromatography HiPrep 26/10 desalting with G-25 Superfine Sephadex as stationary phase in the column. In order to detect the presence of COS, Thin Layer Chromatography (TLC) method was used, followed by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI–TOF–MS) to detect low concentration of COS. Qualitative and quantitative analysis of salt presence were identified using silver nitrate and Volhard method respectively. Ash content was measured using gravimetric method. Results showed those dialysis and gel filtration chromatographies were successfully remove the most of the salt from COS with efficiency of desalting up to 100%. However, the best desalting technique was gel filtration chromatography HiPrep 26/10 which has more complete COS with various degrees of polymerization present in the result.http://bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/110Chitooligosaccharide, desalting technique, dialysis, gel filtration chromatography |
| spellingShingle | Pujoyuwono Martosuyono Asri Pratitis Alexander Prasetya Elisabeth Kartika Prabawati DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION Squalen Chitooligosaccharide, desalting technique, dialysis, gel filtration chromatography |
| title | DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION |
| title_full | DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION |
| title_fullStr | DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION |
| title_full_unstemmed | DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION |
| title_short | DESALINATION OF CHITOOLOGOSACCAHARIDES USING GEL FILTRATION AND ULTRAFILTRATION |
| title_sort | desalination of chitoologosaccaharides using gel filtration and ultrafiltration |
| topic | Chitooligosaccharide, desalting technique, dialysis, gel filtration chromatography |
| url | http://bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/110 |
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