Engineering yeast with a light-driven proton pump system in the vacuolar membrane
Abstract Background The supply of ATP is a limiting factor for cellular metabolism. Therefore, cell factories require a sufficient ATP supply to drive metabolism for efficient bioproduction. In the current study, a light-driven proton pump in the vacuolar membrane was constructed in yeast to reduce...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02273-1 |
| _version_ | 1797362688608698368 |
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| author | Kaoru M. Daicho Yoko Hirono-Hara Hiroshi Kikukawa Kentaro Tamura Kiyotaka Y. Hara |
| author_facet | Kaoru M. Daicho Yoko Hirono-Hara Hiroshi Kikukawa Kentaro Tamura Kiyotaka Y. Hara |
| author_sort | Kaoru M. Daicho |
| collection | DOAJ |
| description | Abstract Background The supply of ATP is a limiting factor for cellular metabolism. Therefore, cell factories require a sufficient ATP supply to drive metabolism for efficient bioproduction. In the current study, a light-driven proton pump in the vacuolar membrane was constructed in yeast to reduce the ATP consumption required by V-ATPase to maintain the acidification of the vacuoles and increase the intracellular ATP supply for bioproduction. Results Delta rhodopsin (dR), a microbial light-driven proton-pumping rhodopsin from Haloterrigena turkmenica, was expressed and localized in the vacuolar membrane of Saccharomyces cerevisiae by conjugation with a vacuolar membrane-localized protein. Vacuoles with dR were isolated from S. cerevisiae, and the light-driven proton pumping activity was evaluated based on the pH change outside the vacuoles. A light-induced increase in the intracellular ATP content was observed in yeast harboring vacuoles with dR. Conclusions Yeast harboring the light-driven proton pump in the vacuolar membrane developed in this study are a potential optoenergetic cell factory suitable for various bioproduction applications. |
| first_indexed | 2024-03-08T16:11:02Z |
| format | Article |
| id | doaj.art-131897f4c8dd4e83b55013e413846583 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-08T16:11:02Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-131897f4c8dd4e83b55013e4138465832024-01-07T12:55:34ZengBMCMicrobial Cell Factories1475-28592024-01-012311810.1186/s12934-023-02273-1Engineering yeast with a light-driven proton pump system in the vacuolar membraneKaoru M. Daicho0Yoko Hirono-Hara1Hiroshi Kikukawa2Kentaro Tamura3Kiyotaka Y. Hara4Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka396Bio, Inc., University of ShizuokaGraduate Division of Nutritional and Environmental Sciences, University of ShizuokaGraduate Division of Nutritional and Environmental Sciences, University of ShizuokaGraduate Division of Nutritional and Environmental Sciences, University of ShizuokaAbstract Background The supply of ATP is a limiting factor for cellular metabolism. Therefore, cell factories require a sufficient ATP supply to drive metabolism for efficient bioproduction. In the current study, a light-driven proton pump in the vacuolar membrane was constructed in yeast to reduce the ATP consumption required by V-ATPase to maintain the acidification of the vacuoles and increase the intracellular ATP supply for bioproduction. Results Delta rhodopsin (dR), a microbial light-driven proton-pumping rhodopsin from Haloterrigena turkmenica, was expressed and localized in the vacuolar membrane of Saccharomyces cerevisiae by conjugation with a vacuolar membrane-localized protein. Vacuoles with dR were isolated from S. cerevisiae, and the light-driven proton pumping activity was evaluated based on the pH change outside the vacuoles. A light-induced increase in the intracellular ATP content was observed in yeast harboring vacuoles with dR. Conclusions Yeast harboring the light-driven proton pump in the vacuolar membrane developed in this study are a potential optoenergetic cell factory suitable for various bioproduction applications.https://doi.org/10.1186/s12934-023-02273-1VacuoleRhodopsinLightATPYeastEngineering biology |
| spellingShingle | Kaoru M. Daicho Yoko Hirono-Hara Hiroshi Kikukawa Kentaro Tamura Kiyotaka Y. Hara Engineering yeast with a light-driven proton pump system in the vacuolar membrane Microbial Cell Factories Vacuole Rhodopsin Light ATP Yeast Engineering biology |
| title | Engineering yeast with a light-driven proton pump system in the vacuolar membrane |
| title_full | Engineering yeast with a light-driven proton pump system in the vacuolar membrane |
| title_fullStr | Engineering yeast with a light-driven proton pump system in the vacuolar membrane |
| title_full_unstemmed | Engineering yeast with a light-driven proton pump system in the vacuolar membrane |
| title_short | Engineering yeast with a light-driven proton pump system in the vacuolar membrane |
| title_sort | engineering yeast with a light driven proton pump system in the vacuolar membrane |
| topic | Vacuole Rhodopsin Light ATP Yeast Engineering biology |
| url | https://doi.org/10.1186/s12934-023-02273-1 |
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