Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants
We obtained transgenic rice (Oryza sativa L. cv. Nipponbare) plants with the gene for maize sucrose-phosphate synthase (EC 2.4.1.14, SPS). Some of the transgenic plants over-expressed maize SPS (over-expressing plants) and some had reduced levels of native SPS protein (co-suppressed plants). There w...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-01-01
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| Series: | Plant Production Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1626/pps.2.172 |
| _version_ | 1819268017087840256 |
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| author | Kiyomi Ono Ken Ishimaru Naohiro Aoki Sakiko Takahashi Kenjiroh Ozawa Yasunobu Ohkawa Ryu Ohsugi |
| author_facet | Kiyomi Ono Ken Ishimaru Naohiro Aoki Sakiko Takahashi Kenjiroh Ozawa Yasunobu Ohkawa Ryu Ohsugi |
| author_sort | Kiyomi Ono |
| collection | DOAJ |
| description | We obtained transgenic rice (Oryza sativa L. cv. Nipponbare) plants with the gene for maize sucrose-phosphate synthase (EC 2.4.1.14, SPS). Some of the transgenic plants over-expressed maize SPS (over-expressing plants) and some had reduced levels of native SPS protein (co-suppressed plants). There was a positive correlation between the amounts of maize SPS protein and SPS activities. However, apparent Km values for uridine diphosphoglucose (UDPG) were higher in over-expressing plants than in control rice plants. These results suggest that overproduced maize SPS protein was not fully activated. The sucrose contents did not differ significantly between control and over-expressing rice plants, but they were lower in co-suppressed plants than in control plants. The starch contents were negatively and the sucrose/starch ratios were positively correlated with SPS activities. Thus, carbon partitioning in the transgenic rice was changed, even though rice is predominantly a sucrose-former. |
| first_indexed | 2024-12-23T21:26:22Z |
| format | Article |
| id | doaj.art-1318c91a613c4751b0c797a4a06707af |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-23T21:26:22Z |
| publishDate | 1999-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-1318c91a613c4751b0c797a4a06707af2022-12-21T17:30:35ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10081999-01-012317217710.1626/pps.2.17211644368Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice PlantsKiyomi Ono0Ken Ishimaru1Naohiro Aoki2Sakiko Takahashi3Kenjiroh Ozawa4Yasunobu Ohkawa5Ryu Ohsugi6National Institute of Agrobiological ResourcesNational Institute of Agrobiological ResourcesNational Institute of Agrobiological ResourcesNational Institute of Agrobiological ResourcesNational Institute of Agrobiological ResourcesNational Institute of Agrobiological ResourcesNational Institute of Agrobiological ResourcesWe obtained transgenic rice (Oryza sativa L. cv. Nipponbare) plants with the gene for maize sucrose-phosphate synthase (EC 2.4.1.14, SPS). Some of the transgenic plants over-expressed maize SPS (over-expressing plants) and some had reduced levels of native SPS protein (co-suppressed plants). There was a positive correlation between the amounts of maize SPS protein and SPS activities. However, apparent Km values for uridine diphosphoglucose (UDPG) were higher in over-expressing plants than in control rice plants. These results suggest that overproduced maize SPS protein was not fully activated. The sucrose contents did not differ significantly between control and over-expressing rice plants, but they were lower in co-suppressed plants than in control plants. The starch contents were negatively and the sucrose/starch ratios were positively correlated with SPS activities. Thus, carbon partitioning in the transgenic rice was changed, even though rice is predominantly a sucrose-former.http://dx.doi.org/10.1626/pps.2.172Carbon partitioningRice (Oryza sativa L.)Sucrose-phosphate synthase (EC 2.4.1.14)Transgenic plant |
| spellingShingle | Kiyomi Ono Ken Ishimaru Naohiro Aoki Sakiko Takahashi Kenjiroh Ozawa Yasunobu Ohkawa Ryu Ohsugi Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants Plant Production Science Carbon partitioning Rice (Oryza sativa L.) Sucrose-phosphate synthase (EC 2.4.1.14) Transgenic plant |
| title | Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants |
| title_full | Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants |
| title_fullStr | Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants |
| title_full_unstemmed | Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants |
| title_short | Characterization of a Maize Sucrose–phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants |
| title_sort | characterization of a maize sucrose phosphate synthase protein and its effect on carbon partitioning in transgenic rice plants |
| topic | Carbon partitioning Rice (Oryza sativa L.) Sucrose-phosphate synthase (EC 2.4.1.14) Transgenic plant |
| url | http://dx.doi.org/10.1626/pps.2.172 |
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