Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium
Abstract Objective This study sought to compare the biocompatibility of a three-dimensional (3D)-printed titanium implant with a conventional machined titanium product, as well as the effect of such implant applied with recombinant human Bone Morphogenetic Protein Type 2 (rhBMP-2) for guided bone...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2021-09-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100440&tlng=en |
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| author | Jaeyoung RYU Hyo-Sun KANG Byung-Hun KANG Seunggon JUNG Min-Suk KOOK Hee-Kyun OH Ji-Yeon JUNG Hong-Ju PARK |
| author_facet | Jaeyoung RYU Hyo-Sun KANG Byung-Hun KANG Seunggon JUNG Min-Suk KOOK Hee-Kyun OH Ji-Yeon JUNG Hong-Ju PARK |
| author_sort | Jaeyoung RYU |
| collection | DOAJ |
| description | Abstract Objective This study sought to compare the biocompatibility of a three-dimensional (3D)-printed titanium implant with a conventional machined titanium product, as well as the effect of such implant applied with recombinant human Bone Morphogenetic Protein Type 2 (rhBMP-2) for guided bone regeneration. Methodology Disk-shaped titanium specimens fabricated either by the conventional machining technique or by the 3D-printing technique were compared by MC3T3-E1 cells cytotoxicity assay. New bone formation was evaluated using a rapid prototype titanium cap applied to the calvaria of 10 rabbits, which were divided into two groups: one including an atelopeptide collagen plug on one side of the cap (group I) and the other including a plug with rhBMP-2 on the other side (group II). At six and 12 weeks after euthanasia, rabbits calvaria underwent morphometric analysis through radiological and histological examination. Results Through the cytotoxicity assay, we identified a significantly higher number of MC3T3-E1 cells in the 3D-printed specimen when compared to the machined specimen after 48 hours of culture. Moreover, morphometric analysis indicated significantly greater bone formation at week 12 on the side where rhBMP-2 was applied when evaluating the upper portion immediately below the cap. Conclusion The results suggest that 3D-printed titanium implant applied with rhBMP-2 enables new bone formation. |
| first_indexed | 2024-04-11T16:53:57Z |
| format | Article |
| id | doaj.art-1318fe7c8e614cfeade1a736916a7b43 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-04-11T16:53:57Z |
| publishDate | 2021-09-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-1318fe7c8e614cfeade1a736916a7b432022-12-22T04:13:19ZengUniversity of São PauloJournal of Applied Oral Science1678-77652021-09-012910.1590/1678-7757-2020-1092Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvariumJaeyoung RYUhttps://orcid.org/0000-0002-2940-5875Hyo-Sun KANGhttps://orcid.org/0000-0001-8901-2283Byung-Hun KANGhttps://orcid.org/0000-0001-6905-3782Seunggon JUNGhttps://orcid.org/0000-0003-2008-3205Min-Suk KOOKhttps://orcid.org/0000-0002-8053-8534Hee-Kyun OHhttps://orcid.org/0000-0003-3103-5815Ji-Yeon JUNGhttps://orcid.org/0000-0002-4419-8077Hong-Ju PARKhttps://orcid.org/0000-0001-7652-5397Abstract Objective This study sought to compare the biocompatibility of a three-dimensional (3D)-printed titanium implant with a conventional machined titanium product, as well as the effect of such implant applied with recombinant human Bone Morphogenetic Protein Type 2 (rhBMP-2) for guided bone regeneration. Methodology Disk-shaped titanium specimens fabricated either by the conventional machining technique or by the 3D-printing technique were compared by MC3T3-E1 cells cytotoxicity assay. New bone formation was evaluated using a rapid prototype titanium cap applied to the calvaria of 10 rabbits, which were divided into two groups: one including an atelopeptide collagen plug on one side of the cap (group I) and the other including a plug with rhBMP-2 on the other side (group II). At six and 12 weeks after euthanasia, rabbits calvaria underwent morphometric analysis through radiological and histological examination. Results Through the cytotoxicity assay, we identified a significantly higher number of MC3T3-E1 cells in the 3D-printed specimen when compared to the machined specimen after 48 hours of culture. Moreover, morphometric analysis indicated significantly greater bone formation at week 12 on the side where rhBMP-2 was applied when evaluating the upper portion immediately below the cap. Conclusion The results suggest that 3D-printed titanium implant applied with rhBMP-2 enables new bone formation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100440&tlng=enThree-dimensional printingBone morphogenetic proteinOsteogenesis |
| spellingShingle | Jaeyoung RYU Hyo-Sun KANG Byung-Hun KANG Seunggon JUNG Min-Suk KOOK Hee-Kyun OH Ji-Yeon JUNG Hong-Ju PARK Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium Journal of Applied Oral Science Three-dimensional printing Bone morphogenetic protein Osteogenesis |
| title | Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium |
| title_full | Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium |
| title_fullStr | Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium |
| title_full_unstemmed | Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium |
| title_short | Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium |
| title_sort | effect of rhbmp 2 applied with a 3d printed titanium implant on new bone formation in rabbit calvarium |
| topic | Three-dimensional printing Bone morphogenetic protein Osteogenesis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100440&tlng=en |
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