Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents
The objective of this study was to investigate the effects of inoculating two bacteriocin-producing strains, <i>Lactiplantibacillus plantarum</i> ATCC14917 and LP1-4, at ensiling on the in vitro ruminal fermentation characteristics and methane production of alfalfa silage with two dry ma...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/3/384 |
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| author | Ziqian Li Fuhou Li Zohreh Akhavan Kharazian Xusheng Guo |
| author_facet | Ziqian Li Fuhou Li Zohreh Akhavan Kharazian Xusheng Guo |
| author_sort | Ziqian Li |
| collection | DOAJ |
| description | The objective of this study was to investigate the effects of inoculating two bacteriocin-producing strains, <i>Lactiplantibacillus plantarum</i> ATCC14917 and LP1-4, at ensiling on the in vitro ruminal fermentation characteristics and methane production of alfalfa silage with two dry matter (DM)contents. Before ensiling, fresh alfalfa was wilted to a moderate DM content (355 g/kg) and a high DM content (428 g/kg). The wilted alfalfa was treated with (1) distilled water (control), (2) commercial strain <i>L. plantarum</i> MTD/1 (MTD/1), (3) bacteriocin-producing <i>L. plantarum</i> ATCC14917 (ATCC14917), and (4) a bacteriocin-like substance producing <i>L. plantarum</i> LP1-4 (LP1-4) at 1 × 10<sup>5</sup> colony forming units (CFU)/g fresh weight. After 90 d of ensiling, the silages were used for in vitro rumen fermentation. Inoculation with the two bacteriocin-producing strains at ensiling remarkably reduced (<i>p</i> < 0.05) in vitro ruminal CH<sub>4</sub> production and enhanced DM digestibility compared with the control group regardless of DM content. For silages with high DM content, inoculation with the bacteriocin-producing strains even increased (<i>p</i> < 0.05) in vitro ruminal total volatile fatty acid production. Therefore, the bacteriocin-producing inoculants have a great potential to mitigate ruminal methane emission but without an adverse effect on rumen fermentation of the inoculated alfalfa silage. |
| first_indexed | 2024-03-11T09:53:41Z |
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| id | doaj.art-1322532338e1450fbc2863ad3722e939 |
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| language | English |
| last_indexed | 2024-03-11T09:53:41Z |
| publishDate | 2023-01-01 |
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| series | Animals |
| spelling | doaj.art-1322532338e1450fbc2863ad3722e9392023-11-16T16:00:08ZengMDPI AGAnimals2076-26152023-01-0113338410.3390/ani13030384Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter ContentsZiqian Li0Fuhou Li1Zohreh Akhavan Kharazian2Xusheng Guo3State Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730013, ChinaState Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730013, ChinaState Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730013, ChinaState Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730013, ChinaThe objective of this study was to investigate the effects of inoculating two bacteriocin-producing strains, <i>Lactiplantibacillus plantarum</i> ATCC14917 and LP1-4, at ensiling on the in vitro ruminal fermentation characteristics and methane production of alfalfa silage with two dry matter (DM)contents. Before ensiling, fresh alfalfa was wilted to a moderate DM content (355 g/kg) and a high DM content (428 g/kg). The wilted alfalfa was treated with (1) distilled water (control), (2) commercial strain <i>L. plantarum</i> MTD/1 (MTD/1), (3) bacteriocin-producing <i>L. plantarum</i> ATCC14917 (ATCC14917), and (4) a bacteriocin-like substance producing <i>L. plantarum</i> LP1-4 (LP1-4) at 1 × 10<sup>5</sup> colony forming units (CFU)/g fresh weight. After 90 d of ensiling, the silages were used for in vitro rumen fermentation. Inoculation with the two bacteriocin-producing strains at ensiling remarkably reduced (<i>p</i> < 0.05) in vitro ruminal CH<sub>4</sub> production and enhanced DM digestibility compared with the control group regardless of DM content. For silages with high DM content, inoculation with the bacteriocin-producing strains even increased (<i>p</i> < 0.05) in vitro ruminal total volatile fatty acid production. Therefore, the bacteriocin-producing inoculants have a great potential to mitigate ruminal methane emission but without an adverse effect on rumen fermentation of the inoculated alfalfa silage.https://www.mdpi.com/2076-2615/13/3/384<i>Lactiplantibacillus plantarum</i>bacteriocinin vitro rumenmethane emissionsdigestibility |
| spellingShingle | Ziqian Li Fuhou Li Zohreh Akhavan Kharazian Xusheng Guo Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents Animals <i>Lactiplantibacillus plantarum</i> bacteriocin in vitro rumen methane emissions digestibility |
| title | Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents |
| title_full | Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents |
| title_fullStr | Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents |
| title_full_unstemmed | Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents |
| title_short | Effect of Inoculating Two Bacteriocin-Producing <i>Lactiplantibacillus plantarum</i> Strains at Ensiling on In Vitro Rumen Fermentation and Methane Emissions of Alfalfa Silage with Two Dry Matter Contents |
| title_sort | effect of inoculating two bacteriocin producing i lactiplantibacillus plantarum i strains at ensiling on in vitro rumen fermentation and methane emissions of alfalfa silage with two dry matter contents |
| topic | <i>Lactiplantibacillus plantarum</i> bacteriocin in vitro rumen methane emissions digestibility |
| url | https://www.mdpi.com/2076-2615/13/3/384 |
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